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dc.contributor.authorKiryu, Ikunari
dc.contributor.authorde la Peña, Leobert D.
dc.contributor.authorMaeno, Yukio
dc.contributor.editorNakamura, Koji
dc.date.accessioned2023-08-29T02:21:26Z
dc.date.available2023-08-29T02:21:26Z
dc.date.issued2006
dc.identifier.issn1341-710X
dc.identifier.urihttp://hdl.handle.net/10862/6472
dc.description.abstractAquaculture of grouper including orange-spotted grouper suffers losses from viral nervous necrosis (VNN). This disease usually occurs in larval stage resulting in a high rate of mortality. It is conceivable that vertical transmission from subclinically infected broodstock fish to the brood is the major route of infection. However, there are only a few studies regarding the fish subclinically infected with VNN and the evidence for a causal relationship between subclinically infected adult fish and vertical transmission remains tenuous. The purpose of this study is to reveal the distribution of the virus in asymptomatically infected fish. Six healthy broodstocks of orange-spotted groupers with body weights ranging from 5 to 12 kg were used and 14 different organs were collected. Cell culture and PCR methods were used to detect the virus. Additionally, 8 wild juveniles with body weights ranging from 2 to 4 g were also analyzed. In our results, the virus was detected by means of PCR but not by the cell culture method. All the broodstocks and wild juveniles were positive in the PCR test except for one juvenile. The highest detection rate of the virus among organs was the brain. However, the virus gene was also amplified from organs other than the central nervous system (CNS). We hypothesize that the virus was latently retained in the CNS of subclinically infected fish and when the fish immune system was weakened such latent virus was released from CNS to the peripheral organs including the gonad. This mechanism might provoke the vertical transmission infection.en
dc.language.isoenen
dc.publisherJapan International Research Center for Agricultural Sciencesen
dc.relation.ispartofseriesJIRCAS Working Report; No. 44
dc.subjectgroupersen
dc.subjectEpinephelus coioidesen
dc.titleDetection of betanodaviruses from different organs of broodstocks and wild juveniles of orange-spotted grouper, Epinephelus coioidesen
dc.typeBook chapteren
dc.citation.spage53en
dc.citation.epage55en
dc.citation.bookTitleSustainable Production Systems of Aquatic Animals in Brackish Mangrove Areasen
dc.subject.asfabreeding stocken
dc.subject.asfajuvenilesen
dc.subject.asfavirosesen
dc.subject.scientificNameEpinephelus coioidesen
local.subjectGrouperen
local.subjectbroodstocksen
local.subjectwild juvenilesen
local.subjectviral nervous necrosis (VNN)en


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