Browsing by Author "Villar, Corina Belle R."
ArticlePrevalence of monodon baculovirus (MBV) was determined using polymerase chain reaction (PCR) on deoxyribonucleic acid (DNA) extracts from the hepatopancreas of the wild black tiger shrimp, Penaeus monodon collected from 7 sampling sites in the Philippines. These sites are considered as the primary sources of broodstock and spawners used for hatchery operations. MBV was detected from all sites except Palawan during the dry season and Negros Occidental and Bohol during wet season. The prevalence of MBV showed no seasonal, sex and size variations. These results show that MBV is an established viral infection in wild populations of Penaeus monodon in the Philippines. Broodstock collected from the contaminated sites could serve as a reservoir of the virus which could infect the post-larvae used to stock in grow-out ponds.
ArticleLD de la Peña, CR Lavilla-Pitogo, CBR Villar, MG Paner, CD Sombito & GC Capulos -
Diseases of Aquatic Organisms, 2007 - Inter ResearchPrevalence of white spot syndrome virus (WSSV) was determined using polymerase chain reaction (PCR) methodology on DNA extracted from the gills of wild black tiger shrimp Penaeus monodon collected from 7 sampling sites in the Philippines. These 7 sampling sites are the primary sources of spawners and broodstock for hatchery use. During the dry season, WSSV was detected in shrimp from all sites except Bohol, but during the wet season it was not detected in any site except Palawan. None of the WSSV-PCR positive shrimp showed signs of white spots in the cuticle. Prevalence of WSSV showed seasonal variations, i.e. prevalence in dry season (April to May) was higher than in the wet season (August to October). These results suggest that WSSV has already become established in the local marine environment and in wild populations of P. monodon. Thus, broodstock collected during the dry season could serve as the main source of WSSV contamination in shrimp farms due to vertical transmission of the virus in hatcheries.