Browsing by Author "Nacario, Jonathan"
Sustained hormone release. III. Use of gonadotropin releasing hormone analogues to induce multiple spawnings in sea bass, Lates calcarifer Two gonadotropin releasing hormone analogues (GnRHa), [D-Ala6, Pro9-ethylamide] mammalian GnRH and [D-Arg6, Pro9-ethylamide] salmon GnRH, were shown to induce spawning in see bass. A single injection of GnRHa induced one spawning, but multiple (two to four) injections spaced 24 h apart produced one to four spawnings in individual females. More striking were the sea bass that spawned up to five times after an osmotic pump, which releases GnRHa for 14 days, was implanted. A less expensive, but equally effective method was implantation of GnRHa in pellets with a cholesterol-cellulose matrix. Multiple spawnings in a single female were produced by only two pellets implanted at one time; individual fish released up to 7 million eggs with good fertility and hatching rates. Individual sea bass were shown to remature in the same season and produce multiple spawnings in both June and September if stimulated with GnRHa in pellets. Stimulation of multiple spawnings was not clearly different if the mammalian or salmon GnRH analogues were used or if the stimulation occurred at the new or first quarter moon. Finally, an individual male was shown to be capable of fertilizing the eggs of one female for at least four sequential spawnings, although the fertility and hatching rates were higher if two males were placed with a female. In conclusion, pellets, pumps and repeated injections produced multiple spawnings in sea bass, but the pellets were more reliable, cheaper, and less stressful to the fish.