Now showing items 1-3 of 3

    • Article

      Acute hepatopancreatic necrosis disease (AHPND) outbreaks in Penaeus vannamei and P. monodon cultured in the Philippines 

      LD de la Peña, NAR Cabillon, DD Catedral, EC Amar, RC Usero, WD Monotilla, AT Calpe, DD Fernandez & CP Saloma - Diseases of Aquatic Organisms, 2015 - Inter Research
      Acute hepatopancreatic necrosis disease (AHPND) has recently emerged as a serious disease of cultured shrimp. It has also been described as early mortality syndrome (EMS) due to mass mortalities occurring within 20 to 30 d after stocking of ponds with postlarvae. Here, Penaeus vannamei and Penaeus monodon from shrimp farms in the Philippines were examined for the toxin-producing strain of Vibrio parahaemolyticus due to AHPND-like symptoms occurring in marketable size shrimp. In the P. vannamei, histology revealed typical AHPND pathology, such as sloughing of undifferentiated cells in the hepatopancreatic tubule epithelium. Analysis using the IQ2000 AHPND/EMS Toxin 1 PCR test generated 218 bp and 432 bp amplicons confirmative of the toxin-producing strain of V. parahaemolyticus among shrimp sampled from 8 of 9 ponds. In the P. monodon, histology revealed massive sloughing of undifferentiated cells of the hepatopancreatic tubule epithelium in the absence of basophilic bacterial cells. PCR testing generated the 2 amplicons confirmatory for AHPND among shrimp sampled from 5 of 7 ponds. This study confirms the presence of AHPND in P. vannamei and P. monodon farmed in the Philippines and suggests that the disease can also impact late-stage juvenile shrimp.
    • Article

      Mortality of pond-cultured Litopenaeus vannamei associated with acute hepatopancreatic necrosis disease (AHPND) and white spot syndrome virus (WSSV) infection in the Philippines 

      LD de la Peña, NAR Cabillon, EC Amar, DD Catedral, RC Usero, JP Faisan Jr., JI Arboleda, WD Monotilla, AT Calpe, DD Fernandez & CP Saloma - Fish Pathology, 2017 - Japanese Society of Fish Pathology
      Mortalities of up to 60% were observed in pond-cultured Litopenaeus vannamei in Bohol, Philippines. Histopathological examination revealed typical acute hepatopancreatic necrosis disease (AHPND) pathology. PCR test generated 1,269 bp and 230 bp amplicons confirmative for the toxin-producing AHPND strain of Vibrio parahaemolyticus among shrimp sampled from eight ponds. The same samples were subjected to PCR analyses for the presence of other viruses, namely WSSV, IHHNV, IMNV, and TSV. The samples were negative for the viruses except WSSV, which was detected after one-step PCR in six out of eight ponds. These results suggested that shrimp were infected dually with AHPND V. parahaemolyticus and WSSV.
    • Book

      Soft-shell crab production using hatchery-reared mud crab 

      EJ Tobias-Quinitio, GXS Libunao, FD Parado-Estepa & AT Calpe - 2015 - Aquaculture Department, Southeast Asian Fisheries Development Center; Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARRD)
      Series: Aquaculture extension manual; No. 61
      "The production of soft-shell crabs is well established in other Asian countries but its sustainability is already being threatened due to the decreasing mud crab population in the wild where the seedstocks are sourced. In the Philippines, production of soft-shell crabs has only been practiced recently due to lack of seedstock and technology. Sourcing of crablets from the natural environment is not encouraged due to dwindling populations of all sizes of mud crabs. Instead, it is recommended that crablets for soft-shell crab production come from hatcheries. The project on soft-shell mud crab production at SEAFDEC/AQD started in 2012 and was later funded by the Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development of the Department of Science and Technology. The project is adopting the individual crab culture sytem of Thailand and Myanmar in pilot-scale and uses hatchery-reared crablets grown to 60-100 g in earthen ponds and stocked in trays. The pilotscale soft-shell production set-up is showcased at the Dumangas Brackishwater Station of SEAFDEC/AQD using crab boxes available in the country. Various sectors are interested to learn how soft-shell crabs are produced. Hence, the previous manual on Soft-shell Mud Crab Farming by Emilia T. Quinitio and May Myat Noe Lwin published in 2009 was revised to include the recent refinements using hatchery-reared crabs and locally available materials. This manual includes sections on the biology of mud crab, how to set-up the facility, management of soft-shell crab production and the cost and return analysis. We hope that various sectors will benefit from this revised manual" -- Foreword