Now showing items 1-2 of 2

    • Article

      The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus) 

      JD Tan, S Adachi & Y Nagahama - General and Comparative Endocrinology, 1986 - Academic Press
      The effects of human chorionic gonadotropin (HCG), forskolin, cyclic nucleotides, the phosphodiesterase inhibitors IBMX and theophylline, cyanoketone, and cycloheximide on the production of estradiol-17β by isolated ovarian follicles of vitellogenic goldfish (Carassius auratus) were examined using 18-hr incubations. HCG and all test agents which are known to increase intracellular concentrations of cAMP significantly stimulated the production of estradiol-17β. However, dibutyryl cGMP was unable to stimulate estradiol-17β production at any concentration used (1–10 mM). Cyanoketone at a concentration of 1 μg/ml completely blocked forskolin-induced estradiol-17β production. Even in the presence of cyanoketone, however, forskolin stimulated conversion of exogenous testosterone to estradiol-17β in a dose-dependent manner, suggesting the involvement of an adenylate-cyclase system in the induction of aromatase activation by vitellogenic follicles of goldfish. Cycloheximide also completely abolished HCG-induced estradiol-17β production when this inhibitor was added within the first 1 hr after the addition of HCG. These results provide evidence that the stimulation of estradiol-17β by goldfish vitellogenic follicles in response to HCG is dependent upon the synthesis of new protein.
    • Article

      Shift in steroidogenesis in the ovarian follicles of the goldfish (Carassius auratus) during gonadotropin-induced oocyte maturation 

      Y Nagahama, FW Goetz & JD Tan - Development, Growth and Differentiation, 1986 - Japanese Society of Developmental Biologists
      Both partially purified chum salmon gonadotropin and 17α-hydroxyprogesterone stimulated in vitro production of testosterone by postvitellogenic follicles of goldfish (Carassius auratus). Chum salmon gonadotropin further enhanced the conversion of exogenously supplied 17α-hydroxyprogesterone to 17α, 20β-dihydroxy-4-pregnen-3-one. The increased medium concentrations of 17α, 20β-dihydroxy-4-pregnen-3-one were associated with the induction of final oocyte maturation.

      The capacity of postvitellogenic follicles to produce steroids in response to exogenous 17α-hydroxyprogesterone was examined in females at various stages of final oocyte maturation following the administration of human chorionic gonadotropin in vivo combined with elevation of holding temperature. The maximum production of testosterone in response to 17α-hydroxyprogesterone was obtained in follicles from initial controls. In contrast, 17α 20β-diOHprog production was very low in initial controls and markedly increased during oocyte maturation (3–6 hr following injection), followed by a significant decrease in follicles collected at 15 hr. Estradiol-17β production by the follicles was very low at any stages of gonadotropin-induced oocyte maturation. These results suggest that gonadotropin-induced shift in the biosynthetic pathway in the follicle from the secretion of predominantly testosterone to 17α, 20β-dihydroxy-4-pregnen-3-one secretion is a prerequisite step for the induction of oocyte maturation in goldfish.