Browsing by Subject "Iodine"
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Effect of iodine disinfection on the bacterial flora and hatching rate of grouper, Epinephelus coioides eggs at the cleavage and eyed stages -
Bulletin of the European Association of Fish Pathologists, 2001 - European Association of Fish PathologistsIn this study, the effect of iodine disinfection on the bacterial flora and hatching rate of grouper egg at two different stages of development were investigated. The eggs (at cleavage and eyed stages) were soaked for 10 min in sterile seawater (control) and at different free iodine concentrations- 2.5, 5, 10, 15 and 20 ppm. Total bacterial and presumptive Vibrio count, as well as the hatching rate of the treated eggs were determined. Results showed that Vibrios are eliminated by iodine disinfection (2.5-20 ppm) but not by rinsing with sterile seawater. The total bacterial load and hatching rate of eggs decreased as the iodine concentration increased. Iodine concentrations of 15 and 20 ppm were effective in significantly reducing the total bacterial load of grouper egg at both the cleavage and eyed stages. However, at these concentrations the hatching rates were also significantly lower. Results also showed that grouper, Epinephelus coioides, eggs tolerate stress better at the eyed stage than at the cleavage stage.
Bulletin of the European Association of Fish Pathologists, 2003 - European Association of Fish PathologistsIn this study, the developmental stage, iodine concentration and exposure time that will reduce the bacterial load without decreasing the hatching rate for the disinfection of grouper (Epinephelus coioides) egg were established. Results of the study showed that the best stage to disinfect grouper egg is at late neurula stage where the embryo shows twitching movement. The effective and safe concentration that will reduce bacterial load but will not decrease hatching rate for the disinfection of grouper egg is 7.5 ppm free iodine for 10 min. Total bacterial count of eggs disinfected with 7.5 ppm free iodine for 10 min (8.99 x 102 cfu/20 eggs) is significantly lower than the unrinsed/undisinfected eggs (1.99 x 107 cfu/20 eggs).