Browsing by Author "Gallardo, W. G."
GABA enhances reproduction of the rotifer Brachionus plicatilis Muller: application to mass culture Based on the results of individual and batch culture experiments in small volumes, we conducted experiments in larger volumes of 100 mL, 1 L and 10 L to determine: (1) at what phase of population growth would gamma-aminobutyric acid (GABA) treatment be most effective; and (2) whether GABA treatment of concentrated rotifers for several hours before mass culture would also be effective. GABA treatment of rotifer cultures at lag phase significantly enhanced population growth, whereas treatment at log phase had a lesser effect, and treatment at stationary phase had no effect. Addition of GABA to rotifer cultures every 2 days hastened population growth until day 8, but resulted in culture collapse thereafter. To reduce the cost of the use of GABA in mass cultures, GABA treatment may be conducted on concentrated rotifers (100 individuals mL−1) before mass culture. GABA treatment of concentrated rotifers for 24 h and 48 h before mass culture resulted in a significantly higher population density compared with their respective controls (no GABA treatment) on day 4, and on days 4 and 6 respectively.
ArticleTechniques to culture rotifers at high-density (2000 to 20 000 ind./ml) have been developed by utilizing condensed phytoplankton products. Many public hatcheries in Japan have introduced automated systems for culturing and harvesting rotifers. Research has been conducted on the diagnosis of rotifer culture status and chemical treatment for reducing stress on rotifers. Preservation of the resting eggs of rotifers for an extended period or at low temperature (4 °C) for a limited time has become possible. Thus, appropriately sized rotifers can be provided to fish larvae, according to fish species and growth stage. Techniques to identify the genetic status of rotifer strains have yet to be developed. Practices for culturing copepods in Japan have also been summarized.
ArticleGamma-aminobutyric acid (GABA) has been shown to enhance the reproduction of the rotifer Brachionus plicatilis Muller in stressful culture conditions. During the enrichment of rotifers for feeding to marine fish larvae, they are usually stressed as a result of exposure to different marine oils and high population densities. This typically results in decreased rotifer survival, reproduction and swimming activity. In the present study, we used GABA to increase rotifer reproduction and the swimming activity of rotifers in enrichment cultures. GABA treatment 24 h before high density enrichment enhanced reproduction during enrichment culture, but not when carried out simultaneously with enrichment. Swimming activity was not significantly affected by GABA treatment 24 h before or simultaneously with nutrient enrichment.