Browsing by Author "n 50010827"
The effect of starvation and subsequent feeding on the hepatocytes of Chanos chanos (Forsskal) fingerlings and fry Excised liver sections of the milkfish, Chanos chanos, fry and fingerlings were studied by transmission electron microscopy. The hepatocytes underwent marked ultrastructural alterations in response to food deprivation of 10-day starvation for fry and 2 months for the fingerlings. The prominent features characterizing the hepatocytes of starved fish were: a reduction of cell and nucleus size; apparent loss of nucleoli; condensation of chromatin material in fry; loss of stored glycogen; reduction of ER profiles; increase in the number of electron-dense bodies containing large amounts of iron in fingerlings; and an increase in mitochondrial size. These changes were reversible following short periods of re-feeding, i.e. 2 days for fry and 4 days for fingerlings, using natural food for the fry and formulated diet for the fingerlings.
Effects of Chlorella-feeding on larval milkfish, Chanos chanos, as evidenced by histological monitoring Milkfish, Chanos chanos, larvae were found to suffer 100% mortality within 6 days of feeding when reared on Chlorella sp. According to the liver ultrastructure, Chlorella-fed fish underwent starvation. Likewise, no signs of lipid absorption were observed in the intestine of Chlorella-fed larvae. On the other hand, Chlorella-related histological alterations of the enterocytes in the anterior part of the intestine were different from starvation-related alterations. It is concluded that Chlorella-feeding creates a starvation situation for larval milkfish, but the early losses with this diet are due to an additional stress specifically introduced by Chlorella-feeding.