Browsing by Author "Millamena, O. M."
ArticleEM Rodriguez, ET Quinitio, FD Parado-Estepa & OM Millamena -
Asian Fisheries Science, 2001 - Asian Fisheries SocietyThree- to five-day old hatchery-reared megalops (4.0 to 6.4 mg body weight) of the mud crab, Scylla serrata, were cultured to the juvenile stage in 20 m2 net cages installed in brackishwater nursery ponds. To establish a suitable stocking density, megalops were stocked at 10, 20, and 30 ind·m-2 in net cages. Treatments were replicated three times over time. After 30 days of culture, mean survival of juveniles ranged from 48.3 to 53.3% and did not vary significantly (P > 0.05) among the three stocking densities. Similarly, the mean final body weights of juveniles ranging from 2.91 to 3.40 g and mass weights 458.9 to 1066 g did not significantly differ among stocking densities. These results show that stocking of crab megalops directly in net cages in a brackishwater pond is feasible at any of the stocking densities tested.
ArticleCR Lavilla-Pitogo, HS Marcial, SAG Pedrajas, ET Quinitio & OM Millamena -
Asian Fisheries Science, 2001 - Asian Fisheries SocietyTo support studies on the development of broodstock and hatchery technology for mud crabs under the genus Scylla, the SEAFDEC Aquaculture Department maintains captive broodstock in land-based tanks. Disease problems seen in broodstock after being held for three months in these tanks include shell disease due to a combination of fouling organisms and chitinoclastic bacteria, bacterial contamination of the hemolymph, parasitic infestation on the gills and shell, and loss of appendages. Shell disease was manifested as off-white and black patches on the shell, that progressed and became perforations exposing underlying tissues. The hemolymph of a significant number of newly recruited crabs harbored mixed populations of sucrose-fermenting vibrios. Pedunculate cirripedes were found in large numbers both in the gill region and on the shell, boring through and creating perforations in the latter. Nematodes and other saprophytic organisms enter the crab through these perforations. The fouling problems that affect the integrity of the shell are considered to reduce the life span and reproductive potential of captive broodstock under tank conditions; therefore, regular cleaning of the shell is recommended to minimize shell fouling.
Reproductive performance of hatchery-bred donkey's ear abalone, Haliotis asinina, Linne, fed natural and artificial diets Hatchery-bred donkey's ear abalone, Haliotis asinina, Linne broodstock were given diets consisting of natural food, seaweed (SW), Gracilariopsis bailinae, D1; combination of SW and artificial diet (AD), D2; and AD alone, D3. Equal numbers of 1 : 1 female and male abalone were stocked in 24 units, 60 L tanks with eight replicate tanks per dietary treatment. Reproductive performance, e.g. number of spawnings, instantaneous fecundity and egg hatching rates, was monitored over 270 days. The mean number of spawnings was not significantly different among treatments. The mean instantaneous fecundity and percent hatching rates were significantly higher in abalone fed D2 or D3 compared to those given D1. Survival of abalone broodstock fed D1 was, however, significantly higher at 88% than those fed either D2 or D3 at 75%. Fatty acid analysis showed that the n-3/n-6 fatty acid ratios of abalone hepatopancreas reflected those of their diets. Mature abalone ovary had n-3/n-6 fatty acid ratio of 1.3. A higher amount of essential nutrients in the artificial diet such as protein, lipid and the highly unsaturated fatty acids, e.g. 20 : 4n-6, 20 : 5n-3, 22 : 6n-3 in abalone fed D2 or D3, may have influenced the increased reproductive performance.