Browsing by Author "Almendras, Jesus M. E."
A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation Various extenders, containing potassium chloride, sodium chloride, glucose, sodium citrate, Ringer's solution, cow serum and milkfish serum were used to preserve milkfish (Chanos chanos) sperm at near-zero temperatures (0-4°C) and in liquid nitrogen (?196°C). Milkfish serum was a superior extender in both cases. After 5 days, comparatively good motility (> 30%) and fertilizing capacity (6.7-18.9%) were observed in the near-zero liquid samples, while in other extenders, sperm ceased to show motility after 2 days. The fertilization success of 4-5 days cryopreserved sperm averaged 67.5% (n = 2) with milkfish serum, 60.5% (n = 2) with 400 mM glucose, 58.0% (n = 2) with 150 mM sodium chloride, 41.2% (n = 1) with Ringer's solution and 31.9% (n = 2) with cow serum.
ArticleExperiments were conducted to determine the tolerance of Siganus guttatus eggs to salinity changes. In the first run, the female was induced to spawn spontaneously by using human chorionic gonadotropin. The fertilized eggs were transferred to seawater of salinities ranging from 8 to 40‰ either at the blastomere or at the gastrula stage. In the second run, the eggs were stripped from the female and artificially fertilized following the dry method. Results indicated that eggs transferred at gastrula stage were more tolerant to salinity changes than those transferred at the blastomere stage. Hatching occurred at all salinities but was highest at 24‰. Percentage of viable larvae was highest at 24‰ and lowest at 8‰. The larvae that hatched at low salinities were relatively longer than those that hatched at ambient and higher salinities.
BookMM Parazo, LMB Garcia, FG Ayson, AC Fermin, JME Almendras, DM Reyes Jr., EM Avila & JD Toledo - 1998 - Aquaculture Department, Southeast Asian Fisheries Development Center
Series: Aquaculture extension manual; No. 18The manual addresses sea bass (Lates calcarifer) fry production in the hatchery. It describes the principles and practical procedures for rearing sea bass - from eggs until metamorphosis - as practiced by the SEAFDEC Aquaculture Department in Tigbauan, Iloilo, Philippines. It is presented under the following main section headings: General principles; biology; Selecting a suitable site; Design - tank systems, seawater supply system, freshwater supply system, aeration system, other facilities; Broodstock - source of breeders, breeding techniques; Egg collection, transport and hatching; Larval rearing - stocking density, feed types and feeding management, water management size-grading of larvae; Harvest and transport of fry; and, Propagation of larval food - Chorella, Brachionus, Artemia, Moina.