Browsing by Author "Borlongan, Emetrio L."
ArticleMN Bautista, F Parado-Estepa, OM Millamena & EL Borlongan -
The Israeli Journal of Aquaculture-Bamidgeh, 1991 - Society of Israeli Aquaculture and Marine BiotechnologyNatural food in combination with either SEAFDEC formulated or other commercial larvae diets was tested for large scale production of Penaeus monodon postlarvae. Two trials of 3 treatments each, 2 replications of each treatment, were conducted in 10 m3 circular concrete tanks. Shrimps were reared from nauplii to postlarvae. Dietary treatments for trial I included:(a) natural food (NF) alone, (b) NF+ commercial plankton substitute (PS) and (c) NF+ SEAFDEC diet (SD).For trial II, commercial powder diets SP or SMP were added:(a) NF + SP, (b) NF + PS + SP + SMP and (c) NF + SD + SP + SMP. Larvae survival was significantly higher in treatments containing the SEAFDEC diets than in the treatments receiving natural food alone (trial I) or natural food in combination with SP (trial II). Larval development was faster in the group fed the SEAFDEC diet; larvae in these groups metamorphosed into postlarvae an average of 1-2 days earlier than groups fed other diets. The presence of either SP or SMP did not improve the efficiency of the feeds. Results showed that larvae performance was affected by the nutrient composition of the diets and that by using proper feeding techniques and management of water quality, large scale hatchery production of P. monodon using natural food in combination with the SEAFDEC diet or plankton substitute is possible.
Mass production in concrete tanks of sugpo Penaeus monodon Fabricius spawners by eyestalk ablation JH Primavera, E Borlongan & RA Posadas -
Fisheries Research Journal of the Philippines, 1978 - Fisheries Research Society of the PhilippinesTwo batches of 196 and 181 P. monodon females were ablated on one eyestalk in March and July 1977, respectively, and a control batch of 180 unablated females was stocked in July 1977. The females were stocked with males in 120-ton concrete tanks with partial flowthrough of water and fed salted mussel (Modiolus metcalfei ) at 15% body weight daily. Total number of spawnings was 82 for the March-ablated group, 3 for the July-ablated group and 4 for the July unablated group. Maturation and mortality in relation to the moult cycle and tank plus water conditions are discussed.
Notes on the induced maturation and spawning in four-month-old Penaeus monodon Fabricius by eyestalk ablation JH Primavera & E Borlongan -
SEAFDEC Aquaculture Department Quarterly Research Report, 1977 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe ablation technique consisted of making an incision across the eyeball to allow free flow of fluids while holding the prawn under water, squeezing the eyeball contents outwards, and pinching hard the eyestalk tissue. The cut area heals completely in about a week; no application of antibiotics is necessary. Spent spawners were tagged with thin brass rings (Rodriguez, 1976) around the unablated eyestalk for a separate experiment on rematuration. Two spawning yielding approximately 277,000 eggs were obtained three weeks after ablation, followed four days later by two more spawnings with 160,000 eggs; all four spawners weighed more than 100 g. With a hatching rate of 98% and 78% for the first and second batch, respectively, the spawnings produced viable nauplii. Water temperatures as low as 23°C due to a delayed cold spell in March depressed molting; weakened larvae had to be discharged at the mysis stage. Although ovarian development continued, no further spawnings were obtained due mainly to the onset of bacterial and fungal disease. Infection is initiated in injured portions of the exoskeleton, sometimes penetrating right through the muscles to the ovarian tissues. The non-flowthrough conditions and mussel meat feeding led to fouling of the culture water resulting in consecutive mortalities caused by disease. Female P.monodon held in maturation pens were ablated at the age of 15 months (Santiago, et al., 1976); they averaged only 16 g body weight after four months growth in ponds. In another experiment, pond-reared P.monodon females ranging from 50 to 80 g were ablated at approximately seven months (Aquacop, 1977). The present results show a minimum age of four months from postlarve that P.monodon is capable of ovarian development and spawning upon ablation. However, maturation is probably affected by size as well as age - the four-month old females weighed an average of 100 g in contrast to the smaller animals in the earlier experiments.
ArticleJH Primavera & E Borlongan -
Annales de biologie animale, biochimie, biophysique, 1978 - Institut National de la Recherche AgronomiqueSpent Penaeus monodon females were tagged around one eyestalk with numbered brass tags, stocked in a concrete tank with males, and periodically examined for ovarian rematuration over a six-month period (February to August, 1977). The females were either artificially-induced (ablated spawners from concrete tanks and marine pens) or wild spawners. Most of the wild spawners were ablated after spawning; some remained unablated to serve as controls. Out of a total of 349 experimental females, 35 or 10 1% had a second spawning and 5 or 1 4% a third spawning; none of the 74 controls spawned subsequently. The average number of days for maturation and rematuration were: ablation to first spawning, 22 6 days; first spawning to second spawning (for females ablated before the first spawning), 11 days; first spawning to second spawning (for females ablated after the first spawning), 15 days; and second spawning to third spawning, 10 4 days. Fecundity of rematured spawners is comparable to that of first spawners; there is insufficient data on hatching rate and fry survival.
BookP Kungvankij, LB Tiro Jr., BJ Pudadera Jr., IO Potestas, KG Corre, EL Borlongan, GA Talean, LF Bustilo, ET Tech, A Unggui & TE Chua - 1986 - Network of Aquaculture Centres in Asia
Series: Aquaculture extension manual; No. 14Details are given of factors to be taken into account for successful hatchery operation. The following aspects are covered: 1) site selection; 2) hatchery design and construction; 3) life cycle; 4) preparation of broodstock for spawning; 5) larval feed; 6) spawning, hatching and larval rearing facilities; 7) spawner selection and egg collection 8) nauplii hatching and transportation; 9) larval rearing; 10) routine hatching, management; 11) port-larvae nursery; and 12) larval harvesting and transportation.