Browsing by Author "Luhan, Maria Rovilla J."
Growing the reproductive cells (carpospores) of the seaweed, Kappaphycus striatum, in the laboratory until outplanting in the field and maturation to tetrasporophyte Carposporophytes of the seaweed, Kappaphycus striatum, from the wild were made to shed spores in the laboratory and grown in multi-step culture method until they reached maturity. For each succeeding transfer onto increasingly bigger culture vessels, there was a marked increase in the growth of carposporelings. When plantlets were ready for outdoor culture, they were placed in aquaria and concrete tanks and later moved to the sea in net cage and long-line for grow-out culture. Successfully growing sporelings from carposporophytes in the laboratory until they reach market size seems to depend on the stage of sporelings and environmental factors such as photoperiod and temperature. In this study, carpospore progenies (diploids) also matured into tetrasporophytes and haploid progenies showed resistance to higher temperature.
ArticleMRJ Luhan, F Harder & AQ Hurtado -
Philippine Agricultural Scientist, 2006 - College of Agriculture, University of the Philippines Los BañosGracilaria heteroclada Zhang et Xia was grown in filter tanks to determine the growth, agar quality and uptake pattern of nitrogen and to observe the water quality in recirculating water system with seaweed. Rapid uptake of nitrogen in G. heteroclada was observed within the first 24th of culture. Filling up of the nitrogen pools in the cell may have continued until the fifth day, the plants started to increase in weight, proportionate to the uptake rate of nitrogen duing the experiment. G. heteroclada stocked at 1 kg m-2achieved a specific growth rate (SGR) of approximately 10.4% d-1 during 15d of culture, at total ammonia-nitrogen and nitrite-nitrogen levels of 0.03-0.27 mg L-1 and 0.04-0.19mg L-1, respectively, in the filter tank. Approximately 11% of nitrogen in the water was removed by the seaweed.
Book chapterCRK Reddy, NS Yokoya, WTL Yong, MRJ Luhan & AQ Hurtado - In AQ Hurtado, AT Critchley & IC Neish (Eds.), Tropical Seaweed Farming Trends, Problems and Opportunities, 2017 - Springer International PublishingAmong the red algae, Kappaphycus and Eucheuma are the two most commercially important carrageenophytes farmed extensively in Southeast Asian countries; they rank top in production in terms of volume of raw material produced. The farming of carrageenophytes has emerged as a successful enterprise and provides a promising, alternative livelihood option for low-income, coastal communities in a number of countries. In 2014, carrageenophyte production worldwide, surpassed other red seaweeds and was placed at the top of the production rankings with 10.99 million MT wet weight (77% of total production of farmed red seaweed) and Indonesia being a major producer. However, over time, the productivity of the crop (carrageenophytes) has declined in some regions due to sourcing of seedlings from single, selected genetic stocks considered to have higher yield potential which resulted in strain fatigue, or loss of vigour. The incidences of disease and epiphytic infestations are on rise in recent times which have severely affected biomass production, as well as the yield and product quality (carrageenan). In order to circumvent the crop productivity issues arising from clonal propagation, the raising of planting materials from spores, derived through the red algal sexual reproductive cycle, has been initiated to support the sustainability of selected, farmed carrageenophytes. Alternatively, in vitro tissue culture techniques have also been explored to not only rejuvenate the vigour of seedlings (i.e. the out-planting material) but also to seek seedlings resilient to stress, disease and epiphytes to act as an invigorated mother stock. These efforts have succeeded to a great extent in the development of appropriate techniques for explant culture, callus induction, callus sub-culture and regeneration to micro-propagules with improved traits. The present chapter briefly summarizes the developments and success achieved in micro-propagation of Kappaphycus and Eucheuma and also provides pointers to both gaps and priority areas for future research required for the advancement of sustainable farming of these carrageenophytes.