Now showing items 1-2 of 2

    • Conference paper

      Molecular typing and antimicrobial susceptibility of Vibrio parahaemolyticus strains 

      RP Maluping, CR Lavilla-Pitogo, JL Romalde & K Krovacek - In Diseases in Asian Aquaculture VI, 2008 - Fish Health Section, Asian Fisheries Society
      The aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines. Seventeen strains of V. parahaemolyticus isolated from shrimps and from the environments where these shrimps are being cultivated were analyzed by RAPD, ERIC and REP-PCR. Antimicrobial susceptibility of these strains to selected compounds was investigated using broth microdilution method. Results of this work and analysis of similarity among strains using Dice coefficient and unweighted average pair group method have demonstrated genetic variability within the V. parahaemolyticus strains. The RAPD, ERIC and REP-PCR were found to be suitable typing methods for V. parahaemolyticus. They have good discriminative ability and can be used as rapid means of comparing these strains for epidemiological investigation. However, the REP-PCR analysis yielded a relatively small number of products suggesting that the REP sequences may not be widely distributed in the V. parahaemolyticus genome. Results of antimicrobial susceptibility revealed that resistance among the strains was rare. In conclusion, RAPD, ERIC and REP-PCR techniques are useful methods for molecular typing of V. parahaemolyticus strains. To our knowledge this is the first study of this kind carried out on V. parahaemolyticus strains isolated from the Philippines.
    • Article

      Molecular typing of Vibrio parahaemolyticus strains isolated from the Philippines by PCR-based methods 

      RP Maluping, C Ravelo, CR Lavilla-Pitogo, K Krovacek & JL Romalde - Journal of Applied Microbiology, 2005 - Wiley-Blackwell
      Aim: The main aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines.

      Methods and Results: Seventeen strains of V. parahaemolyticus isolated from shrimps (Penaeus monodon) and from the environments where these shrimps are being cultivated were analysed by random amplified polymorphic DNA PCR (RAPD-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and repetitive extragenic palindromic PCR (REP-PCR). The results of this work have demonstrated genetic variability within the V. parahaemolyticus strains that were isolated from the Philippines. In addition, RAPD, ERIC and REP-PCR are suitable rapid typing methods for V. parahaemolyticus. All three methods have good discriminative ability and can be used as a rapid means of comparing V. parahaemolyticus strains for epidemiological investigation. Based on the results of this study, we could say that REP-PCR is inferior to RAPD and ERIC-PCR owing to the fact that it is less reproducible. Moreover, the REP-PCR analysis yielded a relatively small number of products. This may suggests that the REP sequences may not be widely distributed in the V. parahaemolyticus genome.

      Conclusions: Genetic variability within V. parahaemolyticus strains isolated in the Philippines has been demonstrated. The presence of ERIC and REP sequences in the genome of this bacterial species was confirmed. Significance and Impact of the Study:The RAPD, ERIC and REP-PCR techniques are useful methods for molecular typing of V. parahaemolyticus strains. To our knowledge this is the first study of this kind carried out on V. parahaemolyticus strains isolated from the Philippines.