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    • Technical Report

      Artificial fertilization of eggs and early development of the milkfish Chanos chanos (Forskal) 

      H Chaudhuri, JV Juario, JH Primavera, R Mateo, R Samson, ER Cruz, EO Jarabejo & JT Canto Jr. - In Induced spawning, artificial fertilization of eggs and larval rearing of the milkfish Chanos chanos (Forskal) in the Philippines, 1977 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Series: Technical report / SEAFDEC Aquaculture Department; 4
      Hydrated eggs obtained from a female milkfish were artificially fertilized with the milt collected from a male injected with acetone-dried pituitaries of salmon. The fertilized eggs (1.1 to 1.25 mm in diameter) developed normally in seawater in basins and Petri dishes at a salinity of 30-34 ppt and successfully hatched in 25 to 28½ hours at a temperature of 26.4-29.9°C. The yolk was completely absorbed in about 2½ days and at this period many postlarvae died. A few larvae were reared up to 5 days but all died within 6 days. Effects of feeding the postlarvae from the third day with freshly hatched trochophore larvae of oysters obtained from eggs artificially fertilized in the laboratory could not be ascertained.
    • Article

      Notes on the external sex characters of Chanos chanos Forskal spawners 

      H Chaudhuri, J Juario, Samson R. & R Mateo - SEAFDEC Aquaculture Department Quarterly Research Report, 1977 - Aquaculture Department, Southeast Asian Fisheries Development Center
      In this study, the authors did not find any visible differences between the sexes with reference to external features such as coloration, shape of head, snout and operculum, presence of tubercles or nasal pores, length, size and shape as well as any roughness in the various fins. However, the anal region of the mature milkfish exhibits discernible anatomical differences in the male and female. In the males, there are 2 main openings visible externally. These are the anterior anus and the posterior urogenital opening at the tip of the urogenital papilla. Internally, the vasa deferentia (male genital ducts) from the testes join into a common duct about 5-10 mm from the urogenital pore. The urinary pore opens into this common duct from the dorsal side. In addition, there are 2 small pores situated on each side of the base of the urogenital papilla opening ventrally into the coelom. In the females, there are 3 main openings in the anal region instead of 2 as found in the males. The anteriormost opening is the anus followed by the genital pore. The third opening is the urinary pore which is posterior to the genital pore located at the tip of the urogenital papilla.
    • Article

      Observations on artificial fertilization of eggs and embryonic and larval development of milkfish, Chanos chanos (Forskal) 

      H Chaudhuri, JV Juario, JH Primavera, R Samson & R Mateo - Aquaculture, 1978 - Elsevier
      Hydrated eggs obtained from a female milkfish, Chanos chanos, were artificially fertilized with the milt collected from a male injected with acetone-dried pituitaries of salmon. The fertilized eggs (1.1–1.25 mm in diameter) developed normally in seawater in basins and petri dishes at a salinity of 30–34‰, and successfully hatched in 25–28.5 hours at a temperature of 26.4–29.9°C. The yolk was completely absorbed in about 2.5 days and during this period many postlarvae died. A few larvae were reared up to 5 days but all died on the 6th day. Attempts were made to feed the postlarvae with freshly hatched trochophore larvae of oysters obtained from eggs artificially fertilized in the laboratory.