Browsing by Author "Zarate, Jacques M."
Conference paperS Watanabe, JM Zarate, MJH Lebata-Ramos & MFJ Nievales - In CA Hair, TD Pickering & DJ Mills (Eds.), Asia-Pacific tropical sea cucumber aquaculture. Proceedings of an international symposium held in Noumea, New Caledonia, 15-17 February 2011, 2012 - Australian Centre for International Agricultural Research
Series: ACIAR Proceedings; No. 136It is important to accurately evaluate the wellbeing or nutritional condition of organisms when monitoring the wild stock conditions and improvement in aquaculture techniques; however, reliable nutritional condition indexes have not been established for sea cucumbers. In this study, the effects of starvation on condition factor (body weight / body volume), coelomic fluid constituent (protein, carbohydrate and cholesterol) concentrations and coelomic fluid density were analysed in an attempt to establish a method to determine nutritional condition in juvenile sandfish (Holothuria scabra). Body length, breadth and weight of juveniles produced at the sea cucumber hatchery of the Aquaculture Department, Southeast Asian Fisheries Development Center, were measured after anaesthetisation with 2% menthol-ethanol. Coelomic fluid protein level was analysed by the bicinchoninic acid method. Carbohydrate level was analysed by the phenol – sulfuric acid method. Cholesterol level was analysed by the Zak method. Coelomic fluid volume and coelomic fluid weight were measured. Starvation caused a concomitant decrease in body length, breadth and weight, resulting in no net change in the condition factor. This result indicated that condition factor cannot be used as a nutritional condition index. Coelomic fluid constituent level could be measured with a small volume of sample (i.e. 10–20 μL). Although no clear pattern was observed in coelomic fluid protein and cholesterol levels during the starvation trial, carbohydrate level increased, as did coelomic fluid density. These results suggest that coelomic fluid density and carbohydrate level may be used as indexes for nutritional condition of sandfish without sacrificing the animal.
Conference paperDJ Mills, NDQ Duy, MA Juinio-Meñez, CM Raison & JM Zarate - In CA Hair, TD Pickering & DJ Mills (Eds.), Asia-Pacific tropical sea cucumber aquaculture. Proceedings of an international symposium held in Noumea, New Caledonia, 15-17 February 2011, 2012 - Australian Centre for International Agricultural Research
Series: ACIAR Proceedings; No. 136South-East Asia has traditionally been the global centre of production of tropical sea cucumbers for Chinese markets. Early research into culture methods took place outside this region, notably in India, the Pacific region and China. However, recent investment in Holothuria scabra (sandfish) culture has led to some significant advances within this region. The Philippines and Vietnam have been at the forefront of recent efforts, with involvement from substantial national programs and local institutions as well as international donors and scientific organisations. Smaller programs are ongoing in Thailand, Malaysia and Indonesia. Recent advances and simplifications in hatchery techniques are a major step forward, having promoted the development of experimental-scale sea-ranching ventures, and given rise to a small, commercial pond-based culture industry in Vietnam. Technology developments in nursery systems are likely to provide opportunities for culture enterprises in a broader range of environments than is now possible. A major research thrust in the Philippines towards developing cooperative sea-ranching enterprises has demonstrated good potential, and institutional/legislative arrangements to ensure adequate property rights have been tested. Rotational culture with shrimp is proving successful in Vietnam, while the possibility of proximate co-culture of sandfish and shrimp has largely been ruled out. Small-scale experiments in the Philippines raise the possibility of co-culture in ponds with a number of finfish species. Current research directions are looking at diversifying technology to increase success in a range of coastal conditions, better understanding the social and biophysical conditions required for success, and finding ways of effectively scaling-out developed systems and technology.
A preliminary study on the diagnosis of coral reef healthiness and establishment of coral replenishment technology T Azuma, JJR Tan, J Zarate, J Altamirano, J Gatus & F Sotto - In MRR Romana-Eguia, FD Parado-Estepa, ND Salayo & MJH Lebata-Ramos (Eds.), Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production … International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA), 2015 - Aquaculture Department, Southeast Asian Fisheries Development CenterField surveys for coral reef through line-intercept-transect (LIT) and temperature profiling using data-loggers were done at three layers of 5, 10, and 15 m depths in coral reef areas, Nogas Island, Anini-y, Antique, Philippines. Preliminary data based on the LIT survey showed that both coverages of substrates by any type of organism and by Scleractinia decreased in the deeper layers. For Scleractinia, Porites sp. occurred predominantly in all the depth layers with the occurrence decreasing with depth. Temperature fluctuation was largest in the 5 m depth layer, where effects of tidal level were also confirmed. While the average temperature decreased with depth, this did not differ beyond 1°C between 5 and 15 m layers during November 2012 to March 2013. Fragments of the Porites sp. and Acropora sp. were sampled and transferred to aquaria at the Tigbauan Main Station of SEAFDEC/AQD. Acropora sp. sampled from the deepest layer alone showed bleaching and thereafter, a part of the fragments regained the color. Experimental trials to clarify the effects of ocean acidification and warming on the health of the coral using the live fragments of Porites sp. showed decreasing trends in both photosynthetic rates and daily growth rates in acidic condition (pH = 7.6), while decrease of zooxanthellae density was observed under warmer conditions (31°C ) for one month. A new methodology for the determination of density of zooxanthellae was established using the fragments of Porites sp. In this study, the need for studies on several coral communities as well as further basic research on coral biology, particularly, responses to the changing environments are discussed for diagnosis of coral reef healthiness and establishment of effective coral replenishment technology.
The relationship betwen nutritional stress and digestive enzyme activities in sea cucumber Holothuria scabra J Zarate, K Niwa & S Watanabe - In K Tanaka, S Morioka & S Watanabe (Eds.), Sustainable stock management and development of aquaculture technology suitable for Southeast Asia, 2012 - Japan International Research Center for Agricultural Sciences
Series: JIRCAS Working Report; No. 75The sea cucumber Holothuria scabra (sandfish) was studied to determine what digestive enzymes are present, to perform a basic characterization of their activity, and to attempt to correlate enzyme activity with nutritional status of the animal. Enzymes alpha amylase, protease (as well as chymotrypsin), cellulase, mannanase, agarase, and xylanase were detected. The enzymes trypsin, alginate lyase and laminarinase were also tested for, but the presence of trypsin was inconclusive, and no alginate lyase or laminarinase was detected. The pH optimum of protease was pH 5 and that of alpha amylase pH 7. Alpha amylase, protease, chymotrypsin, cellulase and mannanase continued to digest their substrates over time. A 2-week starvation experiment showed changes in alpha amylase and protease levels. No differences in cellulase, mannanase, agarose or xylanase activity were detected as a result of the starvation experiment. Another starvation experiment wherein sandfish intestines were sampled every 3 days for 15 days showed that only alpha amylase levels changed with starvation. All samples had lower alpha amylase activity after the withdrawal of feed compared to the day 0 samples. These results suggest the possibility of the use of alpha amylase activity as an indicator of nutritional status, particularly feed deprivation, in sandfish.
Size measurement and nutritional condition evaluation methods in sandfish (Holothuria scabra Jaeger) S Watanabe, JM Zarate, JG Sumbing, MJH Lebata-Ramos & MF Nievales -
Aquaculture Research, 2012 - WileyThe aims of this study were to establish an accurate size measurement method and a nutritional condition evaluation method of Holothuria scabra (Jaeger). Although 0.5% KCl and 0.05% MgSO4 did not induce anaesthesia, 2% menthol–ethanol for 20 min was found to be effective and harmless. The anaesthetization significantly reduced the coefficient of variation of the mean body length and weight by 68% and 43% respectively. During starvation, body size and weight decreased concomitantly, resulting in an unchanged condition factor (body weight/volume), suggesting that the condition factor cannot be used as an index of nutritional condition. Protein, cholesterol and carbohydrate concentrations in the body fluid were analysed to study the relationship with starvation. As the protein and cholesterol concentrations initially increased and then decreased during the starvation period, it is difficult to use them as an index of nutritional condition. The carbohydrate concentration showed a gradual one-fold increase during 10 days of starvation, and it may be used as a proxy for nutritional condition; however, further physiological studies are needed. Body fluid density and volume relative to body size gradually increased and decreased, respectively, during starvation. These methods may be used to correctly monitor the conditions of H. scabra in studies for aquaculture and stock enhancement techniques.
Utilization of organic waste from black tiger shrimp, Penaeus monodon, by sandfish, Holothuria scabra S Watanabe, JM Zarate, MJH Lebata-Ramos, MFJ Nievales & M Kodama - In K Tanaka, S Morioka & S Watanabe (Eds.), Sustainable stock management and development of aquaculture technology suitable for Southeast Asia, 2012 - Japan International Research Center for Agricultural Sciences
Series: JIRCAS Working Report; No. 75In Southeast Asian countries, a large proportion of shrimp aquaculture has switched its target species from native black tiger shrimp, Penaeus monodon, to exotic P. vannamei because of frequent viral disease outbreaks. One of the causes of disease outbreaks is thought to be poor water and sediment conditions in the shrimp pond, which aggravate disease symptoms. To establish co-culture methods of black tiger shrimp and sandfish, Holothuria scabra, for possible mitigation of shrimp pond eutrophication and prevention of disease outbreaks, laboratory experiments were conducted at the Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC/AQD) in Iloilo, Philippines. A feeding trial of juvenile H. scabra using benthic diatom, Navicula ramossisima, and powdered P. monodon feed showed that H. scabra do not grow with fresh shrimp feed on a hard substrate. A feeding trial with and without sand substrate with shrimp feed as food showed that the substrates enhance the growth of H. scabra. H. scabra juveniles were found to grow with detritus and P. monodon feces as food sources in tanks. It was also shown that addition of ground oyster shell to the sand substrate enhances the growth of H. scabra when fed with N. ramossisima. Thus, these results suggest that H. scabra can grow by feeding on organic matter present in a P. monodon pond and may be used to mitigate organic load in P. monodon ponds.