Now showing items 1-3 of 3

    • Book chapter

      Amino and fatty acid profiles of wild-sourced grouper (Epinephelus coioides) broodstock and larvae 

      VR Alava, FMP Priolo, JD Toledo, JC Rodriguez Jr., GF Quinitio, AC Sa-an, MR de la Peña & RD Caturao - In MA Rimmer, S McBride & KC Williams (Eds.), Advances in grouper aquaculture, 2004 - Australian Centre for International Agricultural Research
      Series: ACIAR Monograph 110
      This study was undertaken to provide information on the levels of amino acids in the muscle, liver and gonad of wild-sourced broodstock and larvae, as well as in neurula eggs and day 35 larvae from a hatchery. The fatty acid composition of grouper broodstock tissues was also determined. Samples were analysed for crude protein, amino acids, total lipids and fatty acid contents. Muscle contained higher levels of crude protein and amino acids than the ovary and liver. At the early maturing stage, the grouper ovarian protein was 73.3% and lipid was 19.3%, indicating the high dietary requirements of these nutrients for ovarian development. The crude protein and amino acid contents in wild-sourced larvae were higher than that in eggs and larvae from the hatchery.
    • Article

      Economic analysis of bottom line and raft monoline culture of Kappaphycus alvarezii var. tambalang in Western Visayas, Philippines 

      GPB Samonte, AQ Hurtado-Ponce & RD Caturao - Aquaculture, 1993 - Elsevier
      A survey was conducted among 72 seaweed (Kappaphycus sp.) farmers in the Western Visayas region, Philippines, from March to July 1990 to assess their culture practices in terms of production and economic efficiency. Yields of 5.8 tons/ha per crop (dry weight) and 7.6 tons/ha per crop (dry weight) were obtained from bottom line and raft monoline methods, respectively. Investment requirement was P27361/ha for bottom line culture, and P56757/ha for raft monoline culture (P25=US$1). The bottom line method was more profitable with net farm income of P33286/ha per crop compared with P26365/ha per crop for the raft monoline method. The bottom line method of culturing seaweed is more cost-efficient compared with the raft monoline method. Production cost averaged P3.32/kg for the former method, and P5.55/kg for the latter method. Return on investment was also higher at 243% for the bottom line method and 93% for raft monoline.
    • Book chapter

      Lipid nutrition studies on grouper (Epinephelus coioides) larvae 

      VR Alava, FMP Priolo, JD Toledo, JC Rodriguez Jr., GF Quinitio, AC Sa-an, MR de la Peña & RD Caturao - In MA Rimmer, S McBride & KC Williams (Eds.), Advances in grouper aquaculture, 2004 - Australian Centre for International Agricultural Research
      Series: ACIAR Monograph 110
      The main objectives of this project were to study the lipid chain transfer from the egg stage through hatching and the patterns of lipid conservation or loss during starvation and feeding of larvae in order to elucidate the lipid metabolism of grouper (Phase 1); to determine the fatty acid composition of highly unsaturated fatty acid (HUFA) boosters and enriched live food organisms to enable the possibility of choosing food organisms that provide various dietary levels and ratios of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and arachidonic acid (ARA, Phase 2); and to determine the effect of Brachionus and Artemia, containing different levels and ratios of DHA:EPA:ARA, on the growth and survival of grouper larvae (Phase 3). Total lipids (TL) of samples were extracted and separated into neutral (NL) and polar lipids (PL). The samples collected in Phase 1 were floating neurula eggs, newly hatched (NHL) and unfed 4-day larvae; larvae fed with live food organisms for 25 and 35 days or starved for 3 days; and wild-sourced larvae starved for a week. In Phase 2, the samples collected were phytoplanktons, Brachionus cultured in phytoplankton for 4 days, Diaphanosoma celebensis and Pseudodiaptomus annandalei. In phase 3, larvae were fed Brachionus until day 14 and at day 25 with Artemia. E. coioides eggs contained high DHA, EPA and ARA, demonstrating their importance in larval development. Larvae primarily spent NL as energy, whereas PL was generally conserved. Wild grouper larvae had higher levels of PL than NL, whereas hatchery-sourced eggs and larvae contained higher levels of NL than PL. Based on the lipid content of wild larvae, high phospholipid diets were essential for larvae survival and normal development. A variety of products were effective in enriching the HUFA content (particularly ratios of DHA, EPA and ARA) of live food organisms. HUFA-enriched live food organisms enhanced the growth, survival and pigmentation in grouper larvae.