Browsing by Author "Huyop, Fahrul"
Biodegradition of monochloroacetic acid by a presumptive Pseudomonas sp. strain R1 bacterium isolated from Malaysian paddy (rice) field SN Ismail, AM Taha, NH Jing, RA Wahab, AA Hamid, RV Pakingking Jr. & F Huyop -
Biotechnology, 2008 - Asian Network for Scientific InformationA bacterial strain tentatively identified as Pseudomonas sp. R1 was isolated from a paddy (rice) field that could degrade monochloroacetic acid (MCA) for concentrations ranging from 5 to 40 mM. Quantitative agreement between the amount of MCA introduced and chloride released was also found. MCA dehalogenase activity in this strain was found to be inducible. Cell-free extracts displayed dehalogenating activity with specific halogenated organic compound with no activity on dichloropropionic acid or monochloropropionic acid. The estimated Km values for MCA was 0.14 mM. The optimal pH range for MCA dehalogenase activity (between pH 6.5 and 8.0), whereas the thermal stability profile stable up to 50 °C. The results of our current study demonstrated the potential use of Pseudomonas sp. R1 as suitable biological agent for biodegradation of MCA in contaminated agricultural area.
Development of a polymerase chain reaction (PCR) assay targeted to the dnaJ gene of Vibrio harveyi, a bacterial pathogen in Asian seabass, Lates calcarifer CMA Caipang, RV Pakingking Jr., MJS Apines-Amar, F Huyop & NB Bautista -
Aquaculture, Aquarium, Conservation and Legislation, 2011 - BiofluxPartial sequence of the dnaJ gene of Vibrio harveyi, which was isolated from diseased juvenile Asian seabass, Lates calcarifer was identified. The partial sequence of dnaJ gene of V. harveyi was 447 bp and shared at least 77% identity at the nucleotide level with the dnaJ gene of other Vibrios. It was distinct from the dnaJ gene of other Vibrios but was closely related with the dnaJ gene of V. rotiferianus and V. campbellii having at least 90% nucleotide identity. PCR primers targeting this gene were designed to detect the pathogen in Asian seabass. The assay was specific to V. harveyi and the limit of detection was 100 pg of genomic DNA ml-1 or 100 fg of bacterial genomic DNA in a PCR reaction. This corresponded to a sensitivity of approximately 20 genome equivalents (GE) of V. harveyi. These results indicate that the dnaJ gene is a good candidate to develop primers for the PCR assay in detecting V.harveyi in fish.