Browsing by Author "Capulos, Geimbo C."
JR Somga, LD de la Peña, CD Sombito, MG Paner, VS Suarnaba, GC Capulos, PI Santa Maria & GL Po -
Bulletin of the European Association of Fish Pathologists, 2010 - European Association of Fish PathologistsIllegally imported koi carp were confiscated at the Ninoy Aquino International Airport (NAIA), Manila, Philippines by the Fisheries Quarantine and Inspection Service Officers of the Bureau of Fisheries and Aquatic Resources (BFAR). The confiscated fish were turned over to the BFAR Fish Health Laboratory where they were held for observation at a water temperature of 28 degree C. After 5 days, some fish were showing abnormal swimming behavior and some had died. The most prominent disease signs in the freshly dead and moribund fish were body ulcerations and pale gills showing white necrotic patches, consistent with the clinical signs of KHV infection. Gills were dissected and fixed in 95% ethanol. All of the samples tested positive for KHV in a 1-step PCR assay. This paper reports the first case of KHV associated mortalities in illegally important koi carp confiscated by the Fisheries Quarantine and Inspection Service Officers of BFAR. This highlights the importance of the quarantine and inspection service s role in preventing the illegal entry of fish into the country and the introduction of exotic aquatic diseases.
ArticlePrevalence of monodon baculovirus (MBV) was determined using polymerase chain reaction (PCR) on deoxyribonucleic acid (DNA) extracts from the hepatopancreas of the wild black tiger shrimp, Penaeus monodon collected from 7 sampling sites in the Philippines. These sites are considered as the primary sources of broodstock and spawners used for hatchery operations. MBV was detected from all sites except Palawan during the dry season and Negros Occidental and Bohol during wet season. The prevalence of MBV showed no seasonal, sex and size variations. These results show that MBV is an established viral infection in wild populations of Penaeus monodon in the Philippines. Broodstock collected from the contaminated sites could serve as a reservoir of the virus which could infect the post-larvae used to stock in grow-out ponds.
LD de la Peña, VS Suarnaba, GC Capulos & MNM Santos -
Bulletin of the European Association of Fish Pathologists, 2011 - European Association of Fish PathologistsViral nervous necrosis (VNN) caused by piscine nodavirus is a devastating disease affecting mainly marine finfish. In the Philippines, VNN was first reported in hatchery-reared grouper (Epinephelus coioides) broodstock in 2001. These broodstock are usually fed with trash fish. It is therefore suspected that contaminated trash fish may be the source of VNN transmission to the broodstock. To confirm the source of contamination, periodic monitoring of the VNN prevalence using RT-PCR was done on different species of trash fish available in the Iloilo Fishing Port Complex and on the wild-caught fish in Panay Gulf. Results showed that most of the trash fish and wild-caught fish were sub-clinically infected or carriers of VNN, and that the virus might have already been established in the environment where they were living. These findings provide strong evidence that trash fish could be the main source of viral contamination in broodstock since they are identified as the only major input in the culture systems. To prevent the transmission of VNN to broodstock through contaminated trash fish, a shift to a broodstock pelleted feed is highly recommended.
ArticleLD de la Peña, CR Lavilla-Pitogo, CBR Villar, MG Paner, CD Sombito & GC Capulos -
Diseases of Aquatic Organisms, 2007 - Inter ResearchPrevalence of white spot syndrome virus (WSSV) was determined using polymerase chain reaction (PCR) methodology on DNA extracted from the gills of wild black tiger shrimp Penaeus monodon collected from 7 sampling sites in the Philippines. These 7 sampling sites are the primary sources of spawners and broodstock for hatchery use. During the dry season, WSSV was detected in shrimp from all sites except Bohol, but during the wet season it was not detected in any site except Palawan. None of the WSSV-PCR positive shrimp showed signs of white spots in the cuticle. Prevalence of WSSV showed seasonal variations, i.e. prevalence in dry season (April to May) was higher than in the wet season (August to October). These results suggest that WSSV has already become established in the local marine environment and in wild populations of P. monodon. Thus, broodstock collected during the dry season could serve as the main source of WSSV contamination in shrimp farms due to vertical transmission of the virus in hatcheries.