Now showing items 1-12 of 12

    • Article

      Acute toxicity of nifurpirinol, a fish chemotherapeutant, to milkfish (Chanos chanos) fingerlings 

      CT Tamse & RQ Gacutan - Bulletin of Environmental Contamination and Toxicology, 1994 - Springer Verlag
      Nifurpirinol (trade name Furanace and originally known as P-7138), is a nitrofuran derivative synthesized by the Dainippon Pharmaceutical Co. Ltd., Japan, and was developed exclusively as a broad-spectrum antibiotic for fish and other aquatic organisms (Shimizu and Takase 1967). It has been shown to have bactericidal and fungicidal action in vitro and in vivo (Shimizu and Takase 1967; Amend and Ross 1970; Pearse et al. 1974; Mitchell and Plumb 1980), and was used because of its excellent potential in controlling prawn diseases (Delves-Broughton 1974; Gacutan and Llobrera 1977).

      Milkfish (Chanos chanos Forsskal) is a widely-reared species and a very important aquaculture food crop in most parts of Southeast Asia. Thus, it was the logical choice as test animal for investigating the LC50 toxicity levels of nifurpirinol (6-hydroxymethyl-2-[2-(5-nitro-2-furyl) vinyl] pyridine) after 96 hr exposure. Changes in the normal gill architecture of milkfish after exposure to the drug were also studied (Tamse et al., in preparation).
    • Article

      Bacteria from seawater used in Penaeus monodon larval cultures 

      AT Llobrera & RQ Gacutan - SEAFDEC Aquaculture Department Quarterly Research Report, 1977 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Bacteria in the seawater used in P. monodon hatchery operations were isolated on Bachmann's agar. The total plate counts in 25 isolations ranged from 1.0 - 5.0 x 102 to 5.1 -10.0 x 105 cells per ml. Out of 124 isolates, 98 (79 percent) were Gram-positive and 26 (21 percent) were Gram-negative. Micrococcus and Staphylococcus were dominant in the former group, while Acinetobacter, Moraxella, Flavobacterium and Alcaligenes were most numerous in the latter. Twenty-nine of the Gram-positive isolates closely resembled Peptostreptococcus, Planococcus, and Pediococcus.
    • Article

      Changes induced in the gills of milkfish (Chanos chanos Forsskål) fingerlings after acute exposure to nifurpirinol (Furanace; P-7138) 

      CT Tamse, RQ Gacutan & AF Tamse - Bulletin of Environmental Contamination and Toxicology, 1995 - Springer Verlag
      The need for a chemotherapeutant used specifically for fish disease became increasingly apparent with intensive fish culture practices, and with the possibility of bacterial resistance against drugs used for human and animal medicine (Austin 1985). With this in mind, Nifurpirinol (trade name Furanace; P-7138) was developed by the Dainippon Pharmaceutical Co., Ltd., Japan, and is currently manufactured in the United States as Prefuran. Studies have proven that the drug is effective against bacterial and fungal pathogens in a wide variety of aquatic animals. Most of the Nifurpirinol studies done in the past have dealt on its antimicrobial activity, tissue uptake, and effective treatment levels ranging from 0.5-2.5 mg/L. The 96-hr median lethal concentration (LC50) to channel catfish (Ictalurus punctatus Rafinesque) has also been determined at 0.945-1.90 mg/L, and at 1.70 mg/L for milkfish, Chanos chanos Forsskaal. However, there have only been two studies that have examined the histological effects on treated fish. Histopathologically, Mitchell et al. (1978) found hypertrophy and hyperplasia of the lamellar epithelium in channel catfish gills exposed to 0.5 mg/L for 4 d or longer at 24 plus or minus 2 degree C, while Amend and Ross (1970) working at 21 plus or minus 1 degree C observed no apparent changes in the gills of coho salmon (Oncorhynchus kisutch) exposed intermittently to 1 mg/L of Nifurpirinol. This paper describes the histological changes observed in the gills of milkfish fingerlings used in static, 96-hr Nifurpirinol toxicity tests. Milkfish was used because of its economic importance as a widely cultured food fish in Asia. The gills were chosen as target organs.
    • Book

      Diseases of penaeid shrimps in the Philippines 

      MCL Baticados, ER Cruz-Lacierda, M de la Cruz, RC Duremdez-Fernandez, RQ Gacutan, CR Lavilla-Pitogo & GD Lio-po - 1990 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Series: Aquaculture extension manual; No. 16
      The manual provides information on the diseases that affect the 3 major species of shrimps culture in the Philippines: Penaeus monodon, P. merguiensis and P. indicus. It includes the common name of the disease, causative agent, species affected, stages affected, gross signs, effects on the host and methods of prevention and treatment.
    • Article

      Effect of furanace on the development of larval stages of Penaeus monodon Fabricius 

      RQ Gacutan & AT Llobrera - SEAFDEC Aquaculture Department Quarterly Research Report, 1977 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Zoea 2 (Z2), Mysis 1 (M1) and Postlarva 1 (P1) of P. monodon artificially spawned in closed-system concrete hatchery tanks were bioassayed for their tolerance to the antibiotic furanace. The setup consisted of four 20-liter capacity plastic basins previously conditioned for 15 days with freshwater in full sunlight. During the experiment, each basin was filled with 5 liters of seawater to which was added filtered Chaetoceros and Brachionus to give densities of 5 . 0-7 . 5 x 10-4 cells/ml and 10-20 individuals/ml, respectively. The following are the properties of the water used throughout the experiments: salinity, 26-32%; pH, 7 . 3-8 . 4; temperature, 25-30 degree C; dissolved oxygen, 4 . 5-8 . 4 ppm; nitrite, 0 . 36-0 . 99 ppm; and ammonia, 0 . 10-0 . 30 ppm. To each basin were added 50 healthy larvae of specific stages of P. monodon. After an initial acclimation of one hour in the medium, preweighed amounts of the antibiotic were added and thoroughly dissolved. The concentrations tested were 1 . 0, 2 . 0 and 3 . 0 ppm. One basin always served as control. After 24 hours of exposure, the surviving population in each basin was counted. The survivors were then examined thoroughly under the microscope for unusual behavior and morphological defects brought about by the exposure. To minimize wide variations in the medium as a result of feeding and other manipulations, the systems were all prepared at 9:00 a.m. each time, and the feeds on two instances, one at 5:00 p.m. and another at 5:00 a.m. Fifteen trials conducted with Z2 showed survival ranges of 68% to 98% with a mean of 77 . 6% in the controls; 32% to 94% with a mean of 65 . 7% at 1 ppm, and 0% to 56% with a mean of 36.5% at 2 ppm. There were no survivors at 3 ppm. Interpolation from the survival-dose curve gave a 24-hr LC50 of approximately 1.6 ppm.
    • Article

      Effects of furanace on Brachionus 

      MC Baticados, RQ Gacutan & PJ Gutierrez - SEAFDEC Aquaculture Department Quarterly Research Report, 1977 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Tiger prawn P.monodon) larvae utilize Brachionus a rotifer, as food in the Zoea 3 and mysis stages when they change from an herbivorous to an omnivorous diet. The present work aims to show the effects of furanace on the population growth of Brachionus. Cultures of Brachionus were obtained and fed with Chlorella at a density of 1-2x10 SUP-6 cells/ml. Five liters of the culture water were placed in each of 4 white, circular, 152x304 mm plastic basins. The mean initial densities of the rotifer ranged from 26 . 5 to 38 . 5 individuals/ml. The concentrations of furanace were 0, 1, 2 and 3 mg /l. The cultures were vigorously aerated. Population growth was observed after 3, 6, and 9 hours of exposure. The cultures were thoroughly mixed before samples were taken to ensure an almost equal distribution of the rotifers in the water. To facilitate the counting of the rotifer, one drop of Lugol s solution was added to each sample. This immobilizes the rotifer as well as stops further reproduction. Individuals with only the lorica left or with badly deformed lorica were considered dead. Population counts were done using a Sedgwick-Rafter counting chamber. Among the different durations of exposure, the percentage survival of the populations in the furanace baths were highest after 3 hr. There were slight increases in the control and 2 mg/l and slight decreases in 1 and 3 mg/l. The differences in the mean densities are statistically insignificant at . 01 significance level. After a 6-hr exposure, the control population reached its peak density with a survival of 89%. Populations in furanace baths decreased to 88 . 5% in both 2 and 3 mg /l followed closely by 87% in 1 mg/l. Again, no statistical differences exist among all the levels. The mean percentage survival in 1 and 2 mg/l increased (89% and 91%, respectively) after a 9-hr expsoure, while those in the control and 3 mg/l decreased to 86 . 5% and 88 . 25%, respectively. There were no marked differences in appearance noted among the individuals in furanace baths and those in the control.
    • Conference paper

      Effects of furanace on the development of larval stages of Penaeus monodon Fabricius 

      RQ Gacutan, AT Llobrera & MCL Baticados - In Proceedings of the Second Biennial Crustacean Health Workshop, 1977 April 20-22, Galveston, Texas, 1979 - Sea Grant College Program, Texas A&M University
      Successful molts and morphological defects in P. monodon zoeae (Z1, Z2, Z3) resulting from a 24-h exposure to 1.0 and 2.0 mg/L furanace in baths of 1.0 and 2.0 mg/L were quantified. Molting was delayed in Z1, but not in Z2 and Z3 at 1.0 mg/L; considerably delayed in all sub-stages at 2.0 mg/L. Morphological defects in the telson, carapace, uropods and pereiopods were observed in high frequency in Z3 after the exposure. These abnormalities did not result in 1.0 mg/L. In Z2, a 6-h exposure is deemed optimum for bath in 1.0 mg/L as gauged from higher survival of larvae after 96 h.
    • Article

      Growth of chaetoceros-calcitrans in seawater fortified with fertilizers 

      RQ Gacutan, M Rosales & F Sunez - Kalikasan: The Philippine Journal of Biology, 1979 - University of the Philippines at Los Baños
    • Article

      Isolation and culture in artificial media of Lagenidium from Penaeus monodon larvae 

      MCL Baticados, GL Po, CR Lavilla & RQ Gacutan - SEAFDEC Aquaculture Department Quarterly Research Report, 1977 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Fungal infection of P. monodon larvae is a problem in hatchery operations. The fungus, which attacks the nauplius to postlarval stages and causes up to 100% mortality, has been tentatively identified as belonging to the genus Lagenidium. This pathogenic organism has recently been isolated and cultured. A description is given of the fungus, and features of its biology and pathology are discussed.
    • Article

      Larval mycosis in Penaeus monodon 

      MCL Baticados, GL Po, CR Lavilla & RQ Gacutan - Kalikasan: The Philippine Journal of Biology, 1979 - University of the Philippines, Los Baños
      A phycomycetous fungus, presumably a Lagenidium, was observed to infect Penaeus monodon larvae. Monitoring of the hatchery in 1976 and 1977 showed that it occurred in 35 out of 51 and 22 out of 37 runs, respectively. So devastating was the infection that whole tank populations had to be discarded after 48 hr.

      Extramatrical tubes are sent out profusely; a discharge vesicle is formed from each tube. The cytoplasmic contents of the hypha then flow into the vesicle, and zoospore formation occurs. Zoospore release is facilitated by rupture of the vesicle after continuous movement of the zoospores. The hyphae in infected larvae measure 2.5-6.3 microns wide. The discharge vesicles are 14.5-25.0 microns in diameter; each one releases 14-32 zoospores 3.8-6.3 microns wide by 5.0-6.3 microns long.
    • Article

      Reduction in Chaetoceros populations by furanace 

      MCL Baticados & RQ Gacutan - SEAFDEC Aquaculture Department Quarterly Research Report, 1977 - Aquaculture Department, Southeast Asian Fisheries Development Center
      One of the most promising prophylactic agents being tested to control Penaeus monodon larval diseases is furanace (6-hydroxymethyl-2 2(5-nitro-2-furyl) vinyl pyridine). To evaluate further its suitability as a chemotherapeutic agent, its effects on the population growth of Chaetoceros calcitrans, the diatom used as feed for the zoeal stages, was examined. Chaetoceros populations of uniform density (initial density in all runs: 130-141x10 -3 cells /ml) were placed in nine white, circular (382 sq cm), plastic basins. The physio-chemical characteristics of the culture water were as follows: salinity, 28 . 5-30 . 0 ppt; pH, 8 . 62-8 . 72; temperature, 23-25 . 5 degree C; dissolved oxygen, 7 . 1-9 . 3 ppm; nitrate, 0 . 03-0 . 07 ppm; and ammonia, 0 . 005-0 . 03 ppm. Preweighed furanace granules were dissolved in the culture water, with resulting concentrations of 1 and 2 mg/l (3 replicates each). A set of replicates without furanace served as the control. Population counts of the diatom were taken after 2, 4, 6, 8, and 10 hr exposures. After 4 hr, the population decreased in all three levels. The population in 2 mg/l furanace showed the lowest count and that in control the highest. The population means are not statistically different from one another. The results of the study show that the furanace causes reductions in Chaetoceros population at all durations of exposure.
    • Article

      A suctorean parasite of Penaeus monodon larvae 

      RQ Gacutan, AT Llobrera, CB Santiago, PJ Gutierrez & G Lio - SEAFDEC Aquaculture Department Quarterly Research Report, 1977 - Aquaculture Department, Southeast Asian Fisheries Development Center
      A new disease caused by a suctorean has been observed in tank-spawned and reared P. monodon larvae. Identification of the etiologic agent pointed to Ephelota gemmipara R. Hertwig, a species commonly found to inhabit hydroid colonies.