Now showing items 1-20 of 21

    • Book

      Development and management of milkfish broodstock 

      OS Reyes, EGT de Jesus-Ayson, BE Eullaran, VL Corre Jr. & FG Ayson - 2015 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Series: Aquaculture extension manual; No. 62
      The manual provides developed and refined techniques for collection and transport of spawned eggs and larvae, as well as larval rearing. It also describes the necessary facilities for maintaining milkfish broodstock.

      Guidelines on transporting broodstock, performing biopsy to determine sex of spawners, collecting and cleaning eggs, packing and transporting eggs to hatchery, incubating and hatching eggs, and packing and transporting of larvae are also provided in the manual.

      The importance of nutritional quality of the diet in relation to the performance of the milkfish broodstock and quality of resulting eggs and larvae is also explained in the manual.

      Broodstock feeds are enriched with vitamin C, beta-carotene, and other nutrients for better reproductive performance of broodstock and better egg and larval quality. It also offers formula to initially estimate the number of spawned eggs and determine the hatching rate.

      The manual guides stakeholders and operators who are interested in setting up breeding facilities for milkfish.
    • Conference paper

      Effect of feeding regimes on growth and survival of bighead carp (Aristichthys nobilis Richardson) fry 

      CB Santiago & OS Reyes - In SS De Silva (Ed.), Fish Nutrition Research in Asia. Proceedings of the Third Asian Fish Nutrition Network Meeting, 1989 - Asian Fisheries Society
      Two five-week feeding trials were undertaken to evaluate growth and survival of bighead carp (Aristichthys nobilis ) fry of 1.9-2.4 mg mean weight reared on various feeding regimes. In Treatment 1, the carp fry were fed with Brachionus alone. In Treatment 2, 3, 4 and 5, the fry were fed with Brachionus for 2, 4, 6 and 10 days, respectively, and then with an artificial diet for the remaining period. The carp fry were fed with the combination of Brachionus and artificial diet in Treatment 6 and with artificial diet alone in Treatment 7. Results showed that the combination of Brachionus and artificial diet was the best feeding regime in enhancing the growth of the bighead carp fry. Mean weights of the fry fed with Brachionus for 2, 4, 6 and 10 days prior to the shifting to artificial diet were similar to that of the fry fed with Brachionus alone or artificial diet alone. There was no distinct trend in survival as a function of feeding regime. However, Brachionus alone gave the highest survival rate in both trials.
    • Article

      Effects of dietary lipid source on reproductive performance and tissue lipid levels of Nile tilapia Oreochromis niloticus (Linnaeus) broodstock 

      CB Santiago & OS Reyes - Journal of Applied Ichthyology, 1993 - Blackwell Publishing
      Nile tilapia were fed diets supplemented with one of the following lipid sources at 5% level: cod liver oil, corn oil, soybean oil, a coconut oil-based cooking oil or a combination of cod liver oil and corn oil (1 : 1). The control diet had no lipid supplement and tad fish meal as a sole protein source. A diet with soybean meal as a protein source was also tested. The number of females that spawned, spawning frequency, number of fry per spawning, and total fry production were increased at varying degrees by the supplemental lipid sources except for the cod liver oil. Fish fed the soybean oil diet tad the best overall reproductive performance over a 24-week period. Fish fed the cod liver oil diet had the highest weight gain but the poorest reproductive performance. The suplemental lipids significantly increased crude fat levels in the liver and ovaries. Both males and females Ld the cod liver oil diet had the highest levels of fat in the liver and muscle. The ratio of total n-6/n-3 fatty acid in the liver, ovaries and testes was influenced by the supplemental lipid sources. It was highest in fish fed either the soybean oil diet, the corn oil diet, or the soybean meal diet and lowest in fish fed the control diet or the cod liver oil diet.
    • Article

      An evaluation of formulated diets for Nile tilapia fingerlings 

      CB Santiago, OS Reyes, MB Aldaba & MA Laron - Fisheries Research Journal of the Philippines, 1986 - Fisheries Research Society of the Philippines
      Nine practical diets were formulated and prepared as dry pellet crumbles. These were fed to two batches of Nile tilapia fingerlings (0.753g initial mean body weigt for trial I, and 0.961g for trial II) at 10% of fish biomass daily for eight weeks in glass aquaria or wooden tanks. Diets 1, 2, 3 and 4 contained 20% crude protein (CP), diets 5 and 6 had 25% CP and diets 7, 8 and 9 had 30% CP.

      Mean weight gains were significantly different (P<0.05) among treatments in trial I and in trial II. There were also significant differents in weight gains of tilapia fingerlings fed diets of the same protein level. Diets with higher protein content did not necessarily produce better growth. Irrespective of the protein level, diets containing 18% or more fish meal (diets 3, 6, 7 and 8) gave higher weight increases compared to those containing 0 and 5% fish meal (diets 1, 2, 4, 5 and 9). Diets with ipil-ipil leaf meal or copra meas as sole or major protein source gave the lowest growth response. Of the nine diets, diet 6 produced the highest weight gain followed closely by diets 7 and 3 in both trials. Diets 6, 7 and 3 contained fish meal, soybean meal, copra meal and rice bran, among others. Feed conversion values were also better for these diets.
    • Article

      Growth and survival of grouper Epinephelus coioides (Hamilton) larvae fed free-living nematode Panagrellus redivivus at first feeding 

      OS Reyes, MN Duray, CB Santiago & M Ricci - Aquaculture International, 2011 - European Aquaculture Society
      The free-living nematode, Panagrellus redivivus, was tested as live food for grouper Epinephelus coioides larvae during the first feeding stage. A series of experiments were conducted to determine the acceptability of the free-living nematodes in grouper larvae at first feeding, the optimum nematode density and the response of the larvae to nutritionally enriched nematode. All experiments were conducted in 200-L conical tanks filled with 150-L filtered seawater and stocked at 15 larvae L−1. Duration of feeding experiments was up to day 21 (experiment 1) and 14 days (experiment 2 and 3). Brachionus plicatilis and Artemia (experiment 1) and Brachionus plicatilis alone (experiment 2 & 3) was used as the control treatment. Observations indicated that the grouper larvae readily fed on free-living nematodes as early as 3 days posthatching, the start of exogenous feeding. Optimum feeding density for the larvae was 75 nematodes ml−1. The enrichment of cod liver oil or sunflower oil influenced the total lipids and n-3 highly unsaturated fatty acids of P. redivivus, which in turn influenced those of the grouper larvae, however, growth and survival of the larvae were not affected (P > 0.05). The results from this investigation showed that the nematode, P. redivivus, can be used as first live food for grouper larvae from the onset of exogenous feeding until they could feed on Artemia nauplii.
    • Book

      Hatchery production of snubnose pompano Trachinotus blochii Lacepede 

      OS Reyes, EGT de Jesus-Ayson, FL Pedroso & MIC Cabanilla - 2014 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Series: Aquaculture extension manual; No. 56
      A 26-page extension manual describing the biology, broodstock acquisition & management, larval rearing, harvest & transport and prevention of diseases & parasites in hatchery production of pompano.
    • Article

      Immunization regimen in Asian sea bass (Lates calcarifer) broodfish: A practical strategy to control vertical transmission of nervous necrosis virus during seed production 

      R Pakingking Jr., EG de Jesus-Ayson, O Reyes & NB Bautista - Vaccine, 2018 - Elsevier
      Outbreaks of viral nervous necrosis (VNN) in Asian sea bass (Lates calcarifer) at the larval stages via vertical transmission of nervous necrosis virus (NNV) from asymptomatic broodfish remain as a major deterrent during seed production. A five-year study was conducted to produce NNV-specific-free sea bass broodfish reared in land-based tanks through an annual immunization regimen with the formalin-inactivated NNV. We primarily immunized (intraperitoneal injection) sea bass juveniles (5 g) and monitored the neutralizing antibody (Nab) titers in the sera of these fish at scheduled intervals post-immunization. Nab titers in the sera of immunized fish peaked at Month 2 (titer: 1:4480 ± 1185) but thereafter gradually declined and significantly dropped (1:260 ± 83) at Month 12 post-primary immunization. Booster immunization of these fish at Month 12 post-immunization led to abrupt increases in Nab titers in booster immunized (B-Im) fish at Month 1 (1:12800 ± 6704) but thereafter declined and dropped at Month 12 (1:480 ± 165) post-booster immunization. The annual booster injections with the inactivated vaccine or L-15 (Unimmunized [U-Im]) were consecutively conducted for 4 years until the fish became sexually mature. Mature fish from both groups were successively induced to spawn twice (1-month interval) via intramuscular injection with luteinizing hormone-releasing hormone analogue (LHRH-a; 100 µg/kg BW). NNV was not detected by RT-PCR in oocytes and milts, and spawned eggs of B-Im fish. In contrast, oocytes and milts, and spawned eggs of U-Im fish were NNV positive. Spawned eggs of B-Im broodfish exhibited Nab titers ranging from 1:192 ± 34 to 1:240 while such was not detected (<1:40) in eggs of U-Im fish. Taken together, current data clearly demonstrate that annual immunization regimen with inactivated NNV vaccine is a pragmatic approach for sustaining immunocompetent sea bass broodfish reared in land-based tanks and circumvent the risk of vertical transmission of NNV from asymptomatic broodfish to their offspring under stress of repetitive spawning.
    • Article

      Influence of feeding rate and diet form on growth and survival of Nile tilapia (Oreochromis niloticus) fry 

      CB Santiago, MB Aldaba & OS Reyes - Aquaculture, 1987 - Elsevier
      Young Nile tilapia (12 mg mean body weight and 11 mm total length) were stocked at a density of 5 fish/l in twelve 50-l aquaria filled with 30 l of tap water. They were fed pellet crumbles containing 35% crude protein at various daily feeding rates expressed as percentages of fish biomass. Mean increases in body weight after 5 weeks were 63, 198, 232 and 228 mg for the 15, 30, 45 and 60% feeding rates, respectively, when ambient temperature ranged from 19 to 21°C. Corresponding survival rates were 53, 85, 87 and 84%. Growth and survival rates were enhanced significantly (P < 0.01) at the 30, 45 and 60% feeding rates.

      Two feeding trials were conducted to compare the growth and survival of fry fed pellet crumbles and an unpelleted form of the same diet. Results showed that growth and feed conversion were similar for both forms of diet. However, the survival rate of fry fed pellet crumbles was significantly higher (P < 0.01) than the survival rate of fry fed the unpelleted diet. Prior pelleting of the formulated diet for the tilapia fry given at 30% to 45% of fish biomass daily ensured high survival, fast growth and efficient feed conversion.
    • Conference paper

      Low-cost production of the marine thraustochytrid isolate, Schizochytrium sp. LEY7 as larval live feed enrichement for the mangrove snapper, Lutjanus sp. 

      G Ludevese-Pascual, M de la Peña, O Reyes & J Tornalejo - In CI Hendry (Ed.), Larvi ’13 – Fish & Shellfish Larviculture Symposium, 2013 - Laboratory of Aquaculture & Artemia Reference Center, Ghent University
      This study demonstrates the potential of low-cost production substrates in mass producing the Schizochytrium sp. LEY7 for maximum DHA production. High biomass yield was achieved by optimizing the culture conditions particularly the incubation temperature and salinity levels. Preliminary data on feeding trials have shown promising results and therefore confirmatory experiments need to be conducted to verify the results obtained.
    • Article

      Milkfish (Chanos chanos) fingerling production in freshwater ponds with the use of natural and artificial feeds 

      CB Santiago, JB Pantastico, SF Baldia & OS Reyes - Aquaculture, 1989 - Elsevier
      Milkfish fry were reared to fingerling size in freshwater ponds. For the first experiment, fish were fed the blue-green algae Oscillatoria inoculated and grown in the ponds, Oscillatoria supplemented with a fishmeal-based formulated diet, and the formulated diet alone. Twelve 50-m2 earthen ponds were prepared to enhance growth of the indigenous natural foods. Acclimated wild milkfish fry were stocked randomly at 90/m2 and were fed for 6 weeks. Milkfish fed the formulated diet alone had a significantly higher (P<0.05) mean weight gain (1.314±0.201 g) than milkfish given the combination of Oscillatoria and formulated diet (0.882±0.230 g). Growth was lowest for fish fed Oscillatoria alone. The feeding treatments in the second experiment were: combination of Spirulina powder and formulated diet, formulated diet alone, and rice bran alone. The stocking rate was equivalent to 91.5–92.5 fry/m2 and feeding lasted for 7 weeks. All feeds promoted some growth but the milkfish fed the formulated diet alone invariably had the highest weight increment (1.504±0.167 g), followed by fish given the feed combination (0.881±0.140 g). Rice bran alone gave the lowest growth response. For both pond experiments, growth trends of the young milkfish were similar to those grown under laboratory conditions. Although survival rates were significantly different in one aquarium experiment, survival rates of milkfish in ponds did not differ significantly (P>0.05) among treatments.
    • Article

      Optimum dietary protein level for growth of bighead carp (Aristichthys nobilis) fry in a static water system 

      CB Santiago & OS Reyes - Aquaculture, 1991 - Elsevier
      Isocalric diets (290 kcal digestible energy/100 g) with protein levels ranging from 20 to 50% in increments of 5% were fed to bighead carp fry (3.8±0.2 mg mean body weight and 9.8±0.1 mm total length) for 7 weeks. Growth in weight and length increased as the protein level of the diet increased from 20 to 30% and decreased as the protein level increased further. Although not significantly different (P>0.05) from those of fry fed the 25% or 35% protein diet, weight gain (250 mg) and increase in total length (15.7 mm) were highest for fry fed the 30% protein diet. Feed conversion ratio, protein efficiency ratio and survival rate did not clearly indicate the required protein level. The protein requirement was determined using a static-water culture system but assessment of the water quality failed to indicate an association between ammonia concentration and protein in the diet. Further research is necessary to determine why high levels of protein resulted in depressed growth.
    • Article

      Protective immunity against viral nervous necrosis (VNN) in brown-marbled grouper (Epinephelus fuscogutattus) following vaccination with inactivated betanodavirus 

      R Pakingking Jr., NB Bautista, EG de Jesus-Ayson & O Reyes - Fish and Shellfish Immunology, 2010 - Academic Press
      Viral nervous necrosis (VNN) caused by betanodaviruses has been recently implicated in serious mortalities of groupers in the grow-out culture system. A safe and effective vaccine against this disease is urgently needed. This study demonstrates that a single intramuscular vaccination with formalin-inactivated Philippine strain of piscine betanodavirus (genotype: redspotted grouper nervous necrosis virus; RGNNV) induces potent immune responses and substantial protective immunity against an intramuscular challenge with the homologous virus in brown-marbled grouper, Epinephelus fuscogutattus, a highly susceptible marine fish species to VNN. Seroneutralization assay conducted on sera of vaccinated fish revealed the occurrence of substantial neutralizing-antibody titers from Days 15 (mean titer 1:800) to 190 (1:400) with the highest titer observed at Day 60 post-vaccination (1:5120). When vaccinated fish were challenged with the homologous virus at Days 15, 30 and 75 post-vaccination, significantly higher survival rates were obtained in these fish compared with their corresponding controls (L-15 injected fish). Abrogation of virus multiplication in all vaccinated survivors was indicated by undetectable virus titers in the brains and kidneys paralleled by significantly high levels of neutralizing antibodies in the sera of these fish. Consecutively, replicates of vaccinated fish that survived betanodavirus challenge at Days 15 and 75 post-vaccination were maintained in flow-through aquaria and rechallenged with the homologous virus 3 and 5 months later, respectively. A significant drop in neutralizing-antibody titers of 3 and 8 folds, respectively, were observed in the sera of Days 15 and 75 post-vaccinated fish assayed before the virus rechallenge. Interestingly, reversion in the levels of neutralizing antibodies to significantly high levels (8–15 folds) were noted in these fish after the virus rechallenge. Taken together, our current data clearly demonstrate that a single administration of the inactivated Philippine strain of betanodavirus vaccine can effectively mount a specific anamnestic response and concomitant long-term protection against VNN in grouper at the grow-out culture system.
    • Article

      Reproductive performance and growth of Nile tilapia (Oreochromis niloticus) broodstock fed diets containing Leucaena leucocephala leaf meal 

      CB Santiago, MB Aldaba, MA Laron & OS Reyes - Aquaculture, 1988 - Elsevier
      The effects of dietary leucaena leaf meal on reproductive performance and growth of Nile tilapia were determined. In the preliminary trial, sexually mature Nile tilapia were fed with a control diet or a test diet which had leucaena leaf meal as the only protein source for 24 weeks. Fish fed with the leucaena diet lost some weight and had significantly low (P<0.05) gonadosomatic index and fry production compared to those fed with the control diet. Subsequently, four isonitrogenous diets (20% crude protein) containing varying amounts of leucaena leaf meal (0, 20, 40 and 80%) were fed to Nile tilapia broodstock. Mean weight gain of the female fish decreased as the level of leucaena leaf meal in the diets increased. Females fed with the 80% leucaena diet invariably lost weight. Mean weight gain of males fed with the control diet and the 20 and 40% leucaena diets did not differ significantly (P>0.05). However, growth of males fed with the 80% leucaena diet was remarkably low. Fry production was highest for those fed with the control diet and the 20% leucaena diet. Fry production decreased slightly in fish fed with the 40% leucaena diet and was significantly low (P<0.05) for those fed with the 80% leucaena diet. The low fry production was preceded by a decrease in body weight of the female fish. However, the gonadosomatic indices of the females and the males were not markedly affected by the diets. On the basis of both fry production and growth, leucaena leaf meal should not exceed 40% of the diet of Nile tilapia broodstock.
    • Article

      Requirements of juvenile marine shrimp, Penaeus monodon (Fabricius) for lysine and arginine 

      OM Millamena, MN Bautista-Teruel, OS Reyes & A Kanazawa - Aquaculture, 1998 - Elsevier
      Feeding experiments were conducted using amino acid test diets to determine the dietary requirements of juvenile Penaeus monodon for lysine and arginine. Two sets of the test diets were prepared. The natural protein was supplied by casein and gelatin. Crystalline l-amino acids were added to provide an amino acid profile similar to shrimp muscle protein except for the test amino acid. One set of experimental diets contained graded levels of lysine at 1.18–3.28% of the diet and another set contained arginine at 0.6–3.0% of the diet. The amino acid mixture was pre-coated with carboxymethylcellulose (CMC) and diets were further bound with CMC, cornstarch, and K–carrageenan to prevent leaching losses of amino acids. Shrimp postlarvae, PL20, with mean weight of 21±0.5 mg, were randomly distributed at 10 shrimp per tank in 40-l fiberglass tanks and reared on the diets for 50–56 days. Growth, survival and feed conversion efficiency were determined at termination of feeding trials and signs of nutritional deficiency noted. Lysine and arginine requirements were determined from relationships between weight gains and dietary lysine and arginine levels as analyzed by the broken-line regression method. The requirement of juvenile P. monodon for lysine was estimated to be 2.08% of the diet or 5.2% of dietary protein while the requirement for arginine was 1.85% of the diet or 5.3% of dietary protein. This information is crucial in formulating cost-effective practical diets for juvenile tiger shrimp.
    • Conference paper

      Response of Nile tilapia (Oreochromis niloticus) fry to diets containing Azolla meal 

      CB Santiago, MB Aldaba, OS Reyes & MA Laron - In RSV Pullin, T Bhukaswan, K Tonguthai & JL Maclean (Eds.), The Second International Symposium on Tilapia in Aquaculture, 16-20 March 1987, Bangkok, Thailand, 1988 - Department of Fisheries, Bangkok, Thailand; International Center for Living Aquatic Resources Management
      Sun-dried Azolla pinnata was ground and incorporated into experimental diets at various levels (8.50, 17.00, 25.46, 34.00 and 42.45% of the diets) to replace fish meal in a control diet isonitrogenously. All feeds contained 35% crude protein and 250 kcal digestible energy/100 g. They were fed to Nile tilapia (Oreochromis niloticus ) fry (mean body weights, 14.9 mg in Experiment I and 11.2 mg in Experiment II) at 45% of fish biomass daily for 7 weeks. Results of the 2 experiments showed that Azolla meal is a suitable component of diets for Nile tilapia fry. Growth increased and feed conversion ratios improved as the level of the dietary Azolla meal increased. Survival rates were not affected by the levels of Azolla in the diets.
    • Book

      Seed production of milkfish Chanos chanos Forsskal 

      O Reyes, B Eullaran & EG Ayson - 2016 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Series: Aquaculture extension manual; No. 63
      A 26-page manual describing the site selection, hatchery design, spawning, larval rearing, natural food production, and economic analysis for milkfish.
    • Book

      Seed production of rabbitfish Siganus guttatus 

      FG Ayson, OS Reyes & EGT de Jesus-Ayson - 2014 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Series: Aquaculture extension manual; No. 59
      This manual is mainly intended to serve as a practical guide to fishfarmers and other stakeholders interested to venture in operating a rabbitfish hatchery. It details site selection, hatchery design & layout, and protocols in broodstock management, spawning, larval rearing, and harvest & transport. It has also a section on natural food production for rabbitfish larvae.
    • Conference paper

      Supplementation of grouper (Epinephelus coioides) weaning diet with different sources of phospholipids 

      OS Reyes & IG Borlongan - In CI Henry, G Van Stappen, M Wille & P Sorgeloos (Eds.), Larvi 2001 : 3rd Fish & Shellfish Larviculture Symposium, Gent, Belgium, September 3-6, 2001, 2001 - European Aquaculture Society
      In a rapidly growing fish, phospholipid requirements are high due to rapid membrane development. At an early stage, larvae cannot synthesize large amounts of phospholipids for membrane development, which should therefore be supplemented into the diet to attain maximum growth. This study determines the effect of supplementation of different sources of phospholipids on the growth and survival of 30 days post hatched (dph) grouper, Epinephelus coioides.
    • Article

      Susceptibility of hatchery-reared snubnose pompano Trachinotus blochii to natural betanodavirus infection and their immune responses to the inactivated causative virus 

      R Pakingking Jr., KI Mori, NB Bautista, EG de Jesus-Ayson & O Reyes - Aquaculture, 2011 - Elsevier
      Mass mortality of snubnose pompano Trachinotus blochii fry exhibiting dark coloration, anorexia, and abnormal swimming behavior was recently documented at the hatchery of the Aquaculture Department of the Southeast Asian Fisheries Development Center, Philippines. Samples of brain tissues were collected from affected fish and processed for RT-PCR amplification and virus isolation in cell culture. Infected E-11 cells exhibited cytopathic effect characteristic of betanodavirus. Histopathology of moribund fish showed pronounced vacuolations in the brain, spinal cord, and retina. An RT-PCR product of approximately 430 bp was amplified from the culture supernatant of betanodavirus-infected E-11 cells and sequenced. Sequencing of the T4 region of the coat protein gene (RNA 2) revealed clustering of the isolated virus within the red-spotted grouper nervous necrosis virus type. The pathogenicity of the isolated betanodavirus in healthy pompano juveniles and fry was determined via intramuscular injection and immersion challenges, respectively. Higher mortality rates were obtained in challenged fish compared with the controls. An inactivated vaccine was subsequently prepared by treating the clarified betanodavirus with formalin. Pompano juveniles intraperitoneally injected with the inactivated vaccine exhibited neutralizing antibodies from days 15 (mean titer 1:240) to 125 (1:560) with the highest titer noted at day 64 (1:2240) post-vaccination. Additionally, pompano fry bath-vaccinated and consequently bath-challenged with betanodavirus at day 35 post-vaccination showed higher survival rate compared with the control, indicating the potential of the inactivated betanodavirus vaccine against VNN in pompano fry and juveniles.
    • Article

      Terrestrial leaf meals or freshwater aquatic fern as potential feed ingredients for farmed abalone Haliotis asinina (Linnaeus 1758) 

      OS Reyes & AC Fermin - Aquaculture Research, 2003 - Blackwell Publishing
      Three terrestrial leaf meals, Carica papaya, Leucaena leucocephala, Moringa oliefera and a freshwater aquatic fern, Azolla pinnata were evaluated as potential ingredients for farmed abalone diet. All diets were formulated to contain 27% crude protein, 13% of which was contributed by the various leaf meals. Fresh seaweed Gracilariopsis bailinae served as the control feed. Juvenile Haliotis asinina (mean body weight=13.4±1.6 g, mean shell length= 38.8±1.4 mm) were fed the diets at 2–3% of the body weight day–1. Seaweed was given at 30% of body weight day–1. After 120 days of feeding, abalone fed M. oliefera, A. pinnata-based diets, and fresh G. bailinae had significantly higher (P<0.01) specific growth rates (SGR%) than abalone fed the L. leucocephala-based diet. Abalone fed the M. oliefera-based diet had a better growth rate in terms of shell length (P<0.05) compared with those fed the L. leucocephala-based diet but not with those in other treatments. Furthermore, protein productive value (PPV) of H. asinina was significantly higher when fed the M. oliefera-based diet compared with all other treatments (P<0.002). Survival was generally high (80–100%) with no significant differences among treatments. Abalone fed the M. oliefera-based diet showed significantly higher carcass protein (70% dry weight) and lipid (5%) than the other treatments. Moringa oliefera leaf meal and freshwater aquatic fern (A. pinnata) are promising alternative feed ingredients for practical diet for farmed abalone as these are locally available year-round in the Philippines.