Browsing by Author "Tornalejo, J."
Adsorption and biomass concentration of thraustochytrid Schizochytrium aggregatum (Goldstein and Belsky) in Bunker C Oil BGS Sarinas, LD Gellada, MLT Torrigue, DN Sibonga, ES Torrato, JG Malagad, JG Feril, LAJ Bondoc, JCA Roncal & JA Tornalejo -
Journal of Environmental Science and Management, 2014 - School of Environmental Science and Management, University of the Philippines Los BañosDiverse array of microorganisms such as bacteria, fungi and protists are involved during oil spill. Each microorganism has its own specific function whether it has to degrade or adsorb hydrocarbons. One important microorganism is the Thraustochytrid that is a fungoid protist and are common in marine and estuarine habitats. Numerous studies existed on the biodegradation and adsorption of Thraustochytrids on various substances but not on Bunker C oil. Thus, this study aimed to determine the adsorption capacity and mean biomass of Thraustochytrids in Bunker C oil using different cell densities measured in grams. All of the three treatments or cell densities (1 x 105 cells ml-1, 1 x 106 cells ml-1 and 1 x 107 cells ml-1) were triplicated and average values were recorded. Oil dispersant was used as a control. It showed that Thraustochytrid with 1 x 107 cells ml-1 showed the highest adsorbed oil (.057 ḡ) among the three cell densities and showed significant difference at p = .01 but comparable to the control (.066 ḡ). In terms of biomass concentration, all cell densities showed no significant difference at p = .01. Thraustochytrid is a promising tool during oil spill because it has the capacity to adsorb oil.
Low-cost production of the marine thraustochytrid isolate, Schizochytrium sp. LEY7 as larval live feed enrichement for the mangrove snapper, Lutjanus sp. G Ludevese-Pascual, M de la Peña, O Reyes & J Tornalejo - In CI Hendry (Ed.), Larvi ’13 – Fish & Shellfish Larviculture Symposium, 2013 - Laboratory of Aquaculture & Artemia Reference Center, Ghent UniversityThis study demonstrates the potential of low-cost production substrates in mass producing the Schizochytrium sp. LEY7 for maximum DHA production. High biomass yield was achieved by optimizing the culture conditions particularly the incubation temperature and salinity levels. Preliminary data on feeding trials have shown promising results and therefore confirmatory experiments need to be conducted to verify the results obtained.
Plankton diversity in ballast water of an inter-island passenger-cargo ship calling the Philippine ports BGS Sarinas, LD Gellada, MM Magramo, LO Baria, DB Tirazona, LRD Sorio & JA Tornalejo -
Asian Journal of Biodiversity, 2014 - Liceo PressNumerous studies have been conducted on ballast water species composition and diversity in other countries but not in the Philippines. Thus, this study aimed to provide baseline information on the plankton diversity in ballast water of the inter-island passenger-cargo vessel calling the ports of Iloilo-Bacolod-Manila Cagayan de Oro, Philippines and vice-versa. Specifically, this study aimed to determine the presence of phytoplankton and zooplankton diversity and species density of this plankton measured in cells/ml using the haemacytometer technique. Composite sampling was employed having one liter of ballast water used per ballast tank. A total number of 15 genera of phytoplankton (diatom) and one genus of zooplankton were recorded. Chroococcus, Nannochloris and Protococcus had the highest cells/ml while Ankistrodesmus, Micromonas and Synedra had the lowest cells/ml. The most common phytoplankton observed in ballast tanks were Nannochloris and Protococcus. Neocalanus (copepod) was present in all ballast tanks except in ballast tank 1 (fore-peak). The phytoplankton and zooplankton composition was found to be non-invasive in nature showing its ubiquity in the marine environment. This study provides an initial assessment or preliminary list of phytoplankton and zooplankton diversity from the ballast water of a passenger-cargo vessel calling the Philippine ports.