Browsing by Author "Lavens, Patrick"
Improved larval survival at metamorphosis of Asian seabass (Lates calcarifer) using ω3-HUFA-enriched live food Asian seabass (Lates calcarifer) larvae were fed Brachionus cultured on Chlorella and, as soon as ingestin was possible, different types of Artemia, i.e., nauplii of the San Francisco Bay (SFB) strain, Great Salt Lake (GSL) strain or GSL nauplii that had been bioencapsulated with an emulsion containing high levels of the ω3-HUFAs (highly unsaturated fatty acids) 20:5 and 22:6. San Francisco Bay Artemia with a good natural fatty acid profile and small body size could be offered earlier than the larger but HUFA-poor Great Salt Lake strain. The poor nutritional quality of the latter, however, could be corrected by enriching the nauplii with an ω3-HUFA emulsion for 24 h, after which time high levels of the ω3-HUFAs 20:5 and 22:6 were obtained. When the Artemia diet offered before metamorphosis included natural or supplemented essential fatty acids, no significant differences in dry weight, length or survival of the fish were noticed, as compared to fish fed the naturally deficient GSL Artemia. Onset of metamorphosis and physiological condition after metamorphosis, however, were influenced by the HUFA content of the ingested prey. Seabass larvae fed SFB or enriched GSL Artemia started metamorphosis on day 19, while those in the non-enriched series never achieved metamorphosis and died of a nutritional deficiency syndrome by day 27. An indication of the physiological condition of the larvae and the early detection of the syndrome was possible by subjecting 21- and 25-day-old larvae to a stress test: abrupt exposure of the larvae to 65-ppt saline water resulted in abundant and early mortality in HUFA-deficient fish larvae. Fry receiving ω3-HUFA-fortified Artemia had a superior physiological condition which was reflected by significantly lower mortality figures in the stress test.
Conference paperP Dhert, M Duray, P Lavens & P Sorgeloos - In R Hirano & I Hanyu (Eds.), The Second Asian Fisheries Forum. Proceedings of the Second Asian Fisheries Forum, 17-22 April 1989, Tokyo, Japan, 1990 - Asian Fisheries SocietyThis paper reports on the progress made at the Tigbauan hatchery of SEAFDEC in the Philippines, with the larviculture of the Asian seabass Lates calcarifer when using w3-HUFA enriched Brachionus and Artemia . In view of the high correlation between mouth size and total larval length, the feeding of different size classes of Artemia can be better programmed. The incorporation of the HUFA s 20:5w3 and 22:6w3 in the live prey Artemia , and possibly Brachionus , greatly improved larval ability to metamorphose, although it did not affect growth nor survival until day 21. However, when 21 day old fry were subjected to a stress test, much higher survival rates were obtained in HUFA-enriched seabass larvae, illustrating their superior physiological condition over fry cultured with non-enriched Brachionus and Artemia . These findings were used to propose an improved feeding strategy for the larviculture of Lates calcarifer .