Browsing by Author "Andrino-Felarca, Karen Grace S."
Improved resistance against White Spot Virus (WSV) infection in tiger shrimp, Penaeus monodon by combined supplementation of peptidoglycan and mannan oligosaccharide (MOS) MJS Apines-Amar, KGS Andrino, EC Amar, RE Cadiz & VL Corre Jr. -
Extreme Life, Biospeology and Astrobiology, 2014 - Bioflux SocietyAn eight-week feeding trial was conducted to evaluate the effects of combined supplementation of peptidoglycan and mannan oligosaccharide (MOS) in tiger shrimp, Penaeus monodon. Tiger shrimp (0.29 ± 0.02 g) were fed diets supplemented with different levels of peptidoglycan + (MOS) as immunostimulants for six (6) and eight (8) weeks. Four (4) experimental diets were formulated to contain 0, 0.1, 0.2, and 0.4% peptidoglycan + MOS. The feeding trial was conducted in 250 L capacity concrete circular tanks (replicated four (4) times) with 20 shrimp per tank. Growth, survival, respiratory burst activity, total hemocyte count (THC), and in vivo resistance to WSV infection were evaluated. Weight gain of the shrimp was significantly higher in the immunostimulant-fed groups compared to the control. However, different levels of the immunostimulants did not differ in their effect on the the growth of the shrimp. On the other hand, respiratory burst activity and total haemocyte count (THC) were significantly higher in the group supplemented with 0.2% peptidoglycan + MOS than the rest of the treatments. Likewise, survival after infection with White Spot Virus (WSV) was significantly increased in the 0.2% peptidoglycan + MOS compared to the other groups. The present results demonstrated that using peptidoglycan and MOS together at 0.2% of the diet improves growth, activates immune responses such as respiratory burst activity and THC in P. monodon and give better protection to the shrimp against WSV infection.
Book chapterKGS Andrino-Felarca, EG Estante & CC Lazado - In CMA Caipang, MBI Bacano-Maningas & FF Fagutao (Eds.), Biotechnological Advances in Shrimp Health Management in the Philippines, 2015 - Research SignpostShrimp is a high-value commodity and one of the major aquaculture species in the world, including the Philippines. The shrimp farming industry is dominated by the black tiger shrimp Penaeus monodon and the Pacific white shrimp, Penaeus vannamei. Intensification in shrimp aquaculture to meet the global demand resulted to several socio-economic and biophysical production bottlenecks. Consequently, the issues besetting the industry had raised several questions on its sustainability. In particular, viral diseases remain a constant threat and a significant concern in many shrimp producing countries especially in the developing world. In this chapter, current knowledge on major viral pathogens affecting shrimp aquaculture in the Philippines is presented and discussed. The discussion is focused on white spot syndrome virus (WSSV), monodon baculovirus (MBV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), hepatopancreatic parvovirus (HPV). yellow head virus (YHV), and taura syndrome virus (TSV). Updates on their clinical signs, transmission and distribution are presented. Records of incidence in the Philippines are provided as well. The second half of the chapter discusses some of the methods how to control viral diseases in shrimp farming with a particular focus on vaccination, biosecurity and diagnostics.