Now showing items 1-2 of 2

    • Conference paper

      Development of a simple, rapid, cost-effective diagnostic kit for WSSV 

      PMT Arabit, ADD Nicolasora, PEZ Go, CMA Caipang & MBB Maningas - In MRR Romana-Eguia, FD Parado-Estepa, ND Salayo & MJH Lebata-Ramos (Eds.), Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production … International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA), 2015 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Shrimp aquaculture is one of the most important sources of income and livelihood in the Philippines. For the past two decades, the white spot syndrome virus (WSSV) has adversely affected the production of the Philippine shrimp industry resulting to losses in revenue. Shrimps infected by the virus experience up to 100% mortality, 3 to 10 days post-infection. One way of controlling the disease is early detection, which remains to be too complicated and inaccessible to shrimp farmers. Being a DNA virus, the first step to WSSV diagnosis is the isolation of high-quality DNA suitable for polymerase chain reaction (PCR) or loop-mediated isothermal amplification (LAMP). Using readily available and affordable reagents, a DNA extraction protocol has been especially developed for rapid WSSV-detection; DNA has been successfully extracted from the pleopods of shrimps and the results were comparable with that of commercially available kits from Promega and Zymoresearch. LAMP has been optimized for WSSV detection in the temperature range of 55°C to 68°C and was shown to be faster and ten times more sensitive than conventional PCR. This study together with a locally fabricated machine, offers a more convenient, practical and efficient way of detecting WSSV, with the advantage of using non-invasive means of obtaining shrimp tissue therefore not losing any shrimp meat in the process.
    • Article

      Production of hatchery-bred early juvenile milkfish (Chanos chanos) in nursery ponds through supplemental feeding 

      CJ Jaspe, MSM Golez, RM Coloso & CMA Caipang - Animal Biology and Animal Husbandry, 2012 - Bioflux Society
      Hatchery-bred early juvenile Milkfish (Chanos chanos Forsskål, 1755) (average weight of 0.45 g) were stocked in a 500 m2 nursery pond at a density of 16 juveniles/m2 during the dry months (March-May). The early juveniles were reared for two months with natural food followed by supplemental feeding. Upon the harvest the fish reached an average weight of 9.30 g and a survival rate of 86.9%. A feed conversion ratio (FCR) of 1.08 was attained, with specific growth rate (SGR) of 4.96%/day. The high survival rate and good production could be attributed to the time of the year when the nursery production trial was conducted. The nursery of milkfish in ponds during the summer months ensures sufficient supply of natural food and stable water quality during the crucial phase in the nursery production. This strategy of rearing early juveniles (<1 g) of milkfish in nursery ponds at high stocking densities using a combination of natural food and supplemental feeding could be one of the alternative approaches in the nursery production of this fish.