Now showing items 1-6 of 6

    • Article

      Acute toxicity of nifurpirinol, a fish chemotherapeutant, to milkfish (Chanos chanos) fingerlings 

      CT Tamse & RQ Gacutan - Bulletin of Environmental Contamination and Toxicology, 1994 - Springer Verlag
      Nifurpirinol (trade name Furanace and originally known as P-7138), is a nitrofuran derivative synthesized by the Dainippon Pharmaceutical Co. Ltd., Japan, and was developed exclusively as a broad-spectrum antibiotic for fish and other aquatic organisms (Shimizu and Takase 1967). It has been shown to have bactericidal and fungicidal action in vitro and in vivo (Shimizu and Takase 1967; Amend and Ross 1970; Pearse et al. 1974; Mitchell and Plumb 1980), and was used because of its excellent potential in controlling prawn diseases (Delves-Broughton 1974; Gacutan and Llobrera 1977).

      Milkfish (Chanos chanos Forsskal) is a widely-reared species and a very important aquaculture food crop in most parts of Southeast Asia. Thus, it was the logical choice as test animal for investigating the LC50 toxicity levels of nifurpirinol (6-hydroxymethyl-2-[2-(5-nitro-2-furyl) vinyl] pyridine) after 96 hr exposure. Changes in the normal gill architecture of milkfish after exposure to the drug were also studied (Tamse et al., in preparation).
    • Article

      Acute toxicity of potassium permanganate to milkfish fingerlings, Chanos chanos 

      ER Cruz & CT Tamse - Bulletin of Environmental Contamination and Toxicology, 1989 - Springer Verlag
      Potassium permanganate (KMnO4) is a strong oxidizing agent and is commonly used in aquatic systems to improve available oxygen, treat infectious diseases and parasites, detoxify fish poisons, and control algae. The following study was undertaken to determine the 24- and 96-h median lethal concentration (LC50) of milk fish (Chanos chanos)) fingerlings to KMnO4. The study was also designed to evaluate the histopathological response of fish tissues to KMnO4 but was reported in another paper (Cruz and Tamse 1986).
    • Article

      Changes induced in the gills of milkfish (Chanos chanos Forsskål) fingerlings after acute exposure to nifurpirinol (Furanace; P-7138) 

      CT Tamse, RQ Gacutan & AF Tamse - Bulletin of Environmental Contamination and Toxicology, 1995 - Springer Verlag
      The need for a chemotherapeutant used specifically for fish disease became increasingly apparent with intensive fish culture practices, and with the possibility of bacterial resistance against drugs used for human and animal medicine (Austin 1985). With this in mind, Nifurpirinol (trade name Furanace; P-7138) was developed by the Dainippon Pharmaceutical Co., Ltd., Japan, and is currently manufactured in the United States as Prefuran. Studies have proven that the drug is effective against bacterial and fungal pathogens in a wide variety of aquatic animals. Most of the Nifurpirinol studies done in the past have dealt on its antimicrobial activity, tissue uptake, and effective treatment levels ranging from 0.5-2.5 mg/L. The 96-hr median lethal concentration (LC50) to channel catfish (Ictalurus punctatus Rafinesque) has also been determined at 0.945-1.90 mg/L, and at 1.70 mg/L for milkfish, Chanos chanos Forsskaal. However, there have only been two studies that have examined the histological effects on treated fish. Histopathologically, Mitchell et al. (1978) found hypertrophy and hyperplasia of the lamellar epithelium in channel catfish gills exposed to 0.5 mg/L for 4 d or longer at 24 plus or minus 2 degree C, while Amend and Ross (1970) working at 21 plus or minus 1 degree C observed no apparent changes in the gills of coho salmon (Oncorhynchus kisutch) exposed intermittently to 1 mg/L of Nifurpirinol. This paper describes the histological changes observed in the gills of milkfish fingerlings used in static, 96-hr Nifurpirinol toxicity tests. Milkfish was used because of its economic importance as a widely cultured food fish in Asia. The gills were chosen as target organs.
    • Article

      Histopathological response of milkfish Chanos chanos Forsskal fingerlings to potassium permanganate 

      ER Cruz & CT Tamse - Fish Pathology, 1986 - Japanese Society of Fish Pathology
      Static 96 h bioassays were conducted on milkfish fingerlings at concentrations ranging from 1.00 to 1.80 mg/l KMnO4. Histopathological analyses of gills, liver, and kidney tissues revealed significant changes even in non-lethal concentrations tested. Damage became severe with increasing concentration and longer exposure to the chemical. Partial to complete recovery was observed in gills, liver, and kidney cells of fish exposed to KMnO4 for 96 h and then maintained in KMnO4-free seawater for 240 h.
    • Article

      Some histological observations on the opaque eyes of milkfish Chanos chanos Forskal 

      CT Tamse, F Piedad-Pascual & MC de la Cruz - Fisheries Research Journal of the Philippines, 1983 - Fisheries Research Society of the Philippines
      In a study on energy-protein requirements of milkfish fingerlings using semi-purified diets, several gross observations were made on individual milk-fish such as fin and tail rot, yellowish coloration of the abdomen, opacity and swollen adipose membrane of the eyes. The latter abnormality occurred four to five weeks after feeding semi-purified diets. Milkfish eyes with the abnormality were processed for histological analysis. Opacity of the cornea and lens and degeneration of the eye tissues, thickening of the corneal epthelium and oedema of the stromal layers were seen. Necrosis of the iris, slight thickening of the lens capsule, detachment and destruction of the retinal layers were also observed.
    • Article

      Survival and some histological changes in Penaeus monodon Fabricius juveniles fed various carbohydrates 

      F Piedad-Pascual, RM Coloso & CT Tamse - Aquaculture, 1983 - Elsevier
      Juveniles of P. monodon Fabricius (initial mean weight 1.76 g) were reared on semipurified diets containing 10 or 40% maltose, sucrose, dextrin, molasses, cassava starch, corn starch or sago palm starch for 6 weeks. Highest survival (56%) was obtained in juveniles fed with a diet containing 10% sucrose. Within 10 days of rearing, complete mortality was observed in prawns fed with higher levels of maltose and molasses. After 6 weeks, among the starches, sago palm starch provided for the best survival at 10% level. There was no relationship between time to death and size of the prawn (r = −0.10). Significant differences were observed between the type, as well as the level, of carbohydrate in the diet on the survival of juvenile prawn. Histopathological changes in the hepatopancreas, gills and exoskeleton of juveniles fed with the various carbohydrates were also studied.