Now showing items 1-5 of 5

    • Article

      Large scale hatchery production of Penaeus monodon using natural food and artificial diets 

      MN Bautista, F Parado-Estepa, OM Millamena & EL Borlongan - The Israeli Journal of Aquaculture-Bamidgeh, 1991 - Society of Israeli Aquaculture and Marine Biotechnology
      Natural food in combination with either SEAFDEC formulated or other commercial larvae diets was tested for large scale production of Penaeus monodon postlarvae. Two trials of 3 treatments each, 2 replications of each treatment, were conducted in 10 m3 circular concrete tanks. Shrimps were reared from nauplii to postlarvae. Dietary treatments for trial I included:(a) natural food (NF) alone, (b) NF+ commercial plankton substitute (PS) and (c) NF+ SEAFDEC diet (SD).For trial II, commercial powder diets SP or SMP were added:(a) NF + SP, (b) NF + PS + SP + SMP and (c) NF + SD + SP + SMP. Larvae survival was significantly higher in treatments containing the SEAFDEC diets than in the treatments receiving natural food alone (trial I) or natural food in combination with SP (trial II). Larval development was faster in the group fed the SEAFDEC diet; larvae in these groups metamorphosed into postlarvae an average of 1-2 days earlier than groups fed other diets. The presence of either SP or SMP did not improve the efficiency of the feeds. Results showed that larvae performance was affected by the nutrient composition of the diets and that by using proper feeding techniques and management of water quality, large scale hatchery production of P. monodon using natural food in combination with the SEAFDEC diet or plankton substitute is possible.
    • Article

      Morphometric relationship of length and weight of giant tiger prawn Penaeus monodon according to life stage, sex and source. 

      JH Primavera, FD Parado-Estepa & JL Lebata - Aquaculture, 1998 - Elsevier
      Regression analysis was performed on length–weight and length–length measurements of Penaeus monodon (n=3270 individuals) including carapace length, body length, total length and body (wet) weight of different life stages (nursery, growout and broodstock), sexes (female and male) and sources (pond and wild). Regression lines differed among the life stages and between the sexes and sources at the broodstock stage. Nursery and growout P. monodon showed a greater weight gain per unit length than broodstock. With bigger females, size dimorphism appeared only at the broodstock stage; males and females showed similar sizes in nursery and growout. Equations (including a, b and r) for interconversions of length and weight are reported for the different stages, sources and sexes.
    • Article

      Seed production of mud crab Scylla serrata juveniles 

      ET Quinitio, FD Parado-Estepa, OM Millamena, E Rodriguez & E Borlongan - Asian Fisheries Science, 2001 - Asian Fisheries Society
      A protocol for the large-scale rearing of the mud crab Scylla serrata juveniles was developed based on the results of small-scale experiments on feeding and water management. This paper also reports the success in producing the second generation (F2) crabs.

      Pond-reared adult S. serrata held in 10 m3 concrete tanks with sand substrates were given fish, mussel, annelids and formulated diet. The zoeae produced were stocked in 1.5 or 10 m3 tanks at 30 to 50 ind-l-1 and fed 10 to 15 Brachionus rotundiformis ml-1, 1 to 5 Artemia sauna nauplii ml-1 and 1.5 to 2.0 g shrimp larval commercial diet-m-3 day. Water was replaced daily at 30 to 50% of the total volume starting day 3. Megalops were nursed until crab stage either in tanks or in net cages installed in ponds. Crabs were fed mussel or small shrimps (Acetes sp).

      Hatching occurred 6 to 12 days after spawning at 26.5 to 30.5°C. A female produced 0.42 to 5.23 x 106 zoeae at a time. Mean survival rate from zoea 1 to 3- to 5-day old megalopa was 2.6 ± 0.8% and 32.8 ± 4.8% from megalopa to crab stage. The development from zoea 1 to megalopa required 16 to 18 days. Cannibalism and luminescent bacteria were identified as the major causes of mortality. Highest mortality was observed during the metamorphosis from zoea 5 to megalopa and megalopa to crab 1. First crab stage was obtained 23 to 25 days after hatching. Sorting the crabs during the nursery period minimized cannibalism.

      Completion of the cycle in captivity was attained in 1997 and 1999 when spawns from pond-reared crabs grew to become the second-generation broodstock. The results point to a minimum age of 7.5 to 9 months at which S. serrata hatched their eggs after rearing from zoea 1.
    • Article

      Simulated transport of Scylla serrata zoeae at various loading densities 

      ET Quinitio & FD Parado-Estepa - Asian Fisheries Science, 2001 - Asian Fisheries Society
      Percent mortality of mud crab Scylla serrata zoeae was determined after 6 h of simulated transport at mobile and stationary conditions at loading densities of 10, 20, 30 and 40 x 103 ind-l-1. Mortality was not significantly different among treatments immediately after transport. Surviving zoeae were stocked in basins, fed with Brachionus rotundiformis and mortality was compared 15 h after transport. There was no significant interaction between loading density and condition (mobile and stationary) of transport (P > 0.05). However, larval mortality varied significantly among densities (P < 0.001) regardless of the condition. A density of 10 x 103 ind-l-1 had the lowest mortality (0.56 ± 0.76%) followed by 20 x 103 (1.28 ± 0.39%), 30 x 103 (4.3 ± 0.25%), and 40 x 103 (4.3 ± 0.31%) ind-l-1. In another experiment, the effect of transport duration was determined at a constant loading density of 10 x 103 ind-l-1 in control (not subjected to packing and transport), shaken and unshaken conditions. Zoea mortality did not differ significantly (P > 0.05) after the 6, 9, and 12 h transport. Regardless of the duration, mortality was lowest in the control (0.41 ± 0.05%) compared to those in the shaken (0.99 ± 0.13%) and unshaken (0.79 ± 0.12%) conditions. Likewise, the condition but not the duration of transport affected larval survival at 15 h post-transport. Mortality was lower in the shaken (1.92 ± 0.22%) than in the unshaken condition (2.46 ± 0.17%). Since mortality is low even at 20 x 103 ind-l-1, this can still be used to transport S. serrata zoeae for 6 h. However, loading density should be reduced to 10 x 103 ind-l-1 for transport duration up to 12 h.
    • Conference paper

      Survival of newly-hatched larvae of Epinephelus malabaricus at different salinity levels. 

      FD Parado-Estepa - In P Lavens, P Sorgeloos, E Jaspers & F Ollevier (Eds.), Larvi '91. Short Communications and Abstracts of Contributions Presented at the International Symposium on Fish and Crustacean Larviculture, 27-30 August 1991, Gent, Belgium, 1991 - European Aquaculture Society. EAS Special Publication No. 15
      The development of an appropriate hatchery culture-technique for grouper Epinephelus malabaricus requires knowledge on the biology of this species. The environmental requirements, a critical aspect of biology, have, however, not yet been investigated. This study specifically deals with the influence of salinity on the survival of newly-hatched larvae.