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    • Article

      Domestication of the mud crab Scylla serrata 

      ET Quinitio, JJ de la Cruz, MRR Eguia, FD Parado-Estepa, GS Pates Jr. & CR Lavilla-Pitogo - Aquaculture International, 2011 - Springer Verlag
      The significant decrease in wild mud crab population highlights the need to manage the resources and domesticate crabs. This paper presents the initial results of the domestication of mud crab Scylla serrata aimed at producing good-quality captive broodstock. The analysis of the genetic structure of the base population was done as a prerequisite for domestication. Adult S. serrata from the northern to southern parts of the Philippines (Cagayan, Camarines, Samar, and Surigao) were obtained for genetic diversity analysis and domestication. Analysis of molecular variance showed that differences in the genetic variability between the four populations were not significant. Moreover, no significant deviation from Hardy–Weinberg Equilibrium was observed in each sample population and even in pooled populations. Body weight was positively correlated with the carapace width. Second spawning occurred 41–46 days after the first spawning and 34 days from second to third spawning. However, there was a decrease in the number of zoea in repeat spawnings. Twenty-four first-generation (F1) families were produced from the four sites. The duration from spawning of the base population (P0) to attainment of broodstock size F1 was 10–14 months. Four second-generation (F2) families were produced after 11–12 months. Up to the F2, crabs tested negative for six viruses: white spot syndrome virus, infectious hypodermal and hematopoietic necrosis virus, gill-associated virus, yellow head virus, Taura syndrome virus, and infectious myonecrosis virus. The reproductive performance of P0 was comparable to the succeeding generations. Several families were obtained from one population in a year. However, due to the cannibalistic behavior of crabs, more space is required for the nursery and grow-out phase. The domestication of S. serrata is the first study done on any mud crab species in the Indo-west Pacific region. The initial results would serve as guide to understand and eliminate the barriers to mud crab domestication. The breeding technology developed from this study will support the production of good-quality seedstock for farming.
    • Conference paper

      Larval rearing of Penaeus monodon: Feeds and feeding techniques 

      FD Parado-Estepa - In RD Fortes, LC Darvin & DL de Guzman (Eds.), Fish and Crustacean Feeds and Nutrition. Proceedings of the Seminar-Workshop on Fish and Crustacean Feeds and Nutrition, 25-26 February 1985, Iloilo City, Philippines, 1989 - Philippine Council for Aquatic and Marine Research and Development
      Larval rearing of P.monodon requires mass culture of selected phytoplankton and zooplankton species. In the protozocal stage, Skelatonoma; Chaetoceros or Tetraselmis is given singly or in combination. The rotifer Brachionus and/or the brine shrimp ArTEMI are added to the diet as larvae metamorphose to the mysis stage. Attempts to replace these organisms with other organisms or with artificial diets are discussed.

      The use of microparticulate diets as supplement to algal food or Artema increases larval survival to 30-70%. The advantages of using this type of diet are enumerated.