Browsing by Author "Franco, Annie Villa"
BookMR de la Peña & AV Franco - 2013 - Aquaculture Department, Southeast Asian Fisheries Development Center
Series: Aquaculture extension manual; No. 55The 32-page extension manual describes the biology, culture techniques and maintenance and mass propagation of 12 phytoplankton species used as live feed in marine hatcheries.
Microalgal paste production of the diatom Chaetoceros calcitrans using electrolytic flocculation method at optimum culture conditions MR de la Peña, AV Franco, HP Igcasan Jr., MDGN Arnaldo, RM Piloton, SS Garibay & VT Balinas -
Aquaculture International, 2018 - Springer VerlagThe optimum culture conditions of the local strain Chaetoceros calcitrans were determined to improve biomass and reduce cost of production. Under outdoor culture conditions, higher cell density was attained when the cultures were enriched with Tungkang Marine Research Laboratory (TMRL) medium composed of cheap technical grade reagents and cultured at 25 g L−1 salinity. The cultures were lighted with two 40 W cool-white GE fluorescent tubes (24–35 μmol photon m−2 s−1). Using semi-continuous culture system under established optimum culture conditions, C. calcitrans can be re-cultured thrice and concentrated at each culture cycle using electrolytic flocculation method to produce 4.6 kg m−3 of diatom paste. The viability of concentrated C. calcitrans after 3 months of storage was comparable to live diatom cells. Simple preservation technique by low-temperature storage is convenient for storing algal concentrates for use as starter cultures and for feeding invertebrates. The paste costs USD 8.24 kg−1 inclusive of the assets and flocculation materials for culturing and harvesting the diatom, respectively. This study established the suitable conditions for mass culture of C. calcitrans and produced concentrated diatoms in paste form that is readily available for aquaculture hatcheries at a lower cost.