Now showing items 1-3 of 3

    • Conference paper

      Flounder metamorphosis: its regulation by various hormones 

      EG de Jesus, T Hirano & Y Inui - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development Center
      The elongation and shortening of the flounder fin rays during its metamorphosis may be parallel to the appearance and resorption of the tadpole tail during amphibian metamorphosis. The dorsal fin rays of the Japanese flounder (Paralichthys olivaceus) elongate during the time when thyroid hormone levels are low and are resorbed during climax of metamorphosis when thyroid levels are elevated. Using an in vitro system for culture of isolated flounder fin rays, we examined how various hormones affect the process of resorption. Both T4 and T3 directly stimulated fin ray shortening, T3 being more potent than T4. Other hormones did not directly affect the resorption process but modified the tissue's response to thyroid hormones. Cortisol enhanced the stimulatory effects of both T4 and T3. On the other hand, estradiol and testosterone were inhibitory. Ovine prolactin (oPRL) also diminished the effect of T3 while ovine growth hormone (oGH) was without effect. Similar observations were observed from the in vivo studies.

      Thyroid hormone levels, especially T4, were low during premetamorphosis, increased during prometamorphosis, to peak levels during metamorphic climax, and declined in the juveniles. The changes in whole body concentrations of Cortisol paralleled the changes in thyroid hormone concentrations. On the other hand, whole body estradiol and testosterone concentrations did not show significant change and remained low throughout the larval period. The expression of GH and PRLmRNA, as assessed by in situ hybridization using cDNA for flounder PRL and GH also increased during the metamorphosis. However, the increase in the expression of GH and PRL genes was observed later than the increases in tissue levels of thyroid hormones and Cortisol. At late climax, the flounder larvae have undergone considerable transformation, presumably triggered by both hormones, hence the increase in PRL could hardly be antimetamorphic but may have other physiological implications.
    • Conference paper

      Mass larval rearing technology of marine finfish in Japan 

      K Fukusho - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development Center
      With economic development and increased demand for high price fish, industrial scale marine finfish culture in Japan was started in 1960-1965 for yellowtail Seriola quinqueradiata. Sustainable supply of wild juvenile and development of floating cage with synthetic fiber net have spurred the culture of nearly 30 species and total production in 1991 is 265 x 103 metric tons (nearly 25% of total aquaculture production). Although salmon ranching had been started in 1888, a national project of ocean ranching was only initiated in 1963 with the present target of 26 species of marine finfish. Ocean ranching aims to increase fisheries resources in coastal sea by stocking hatchery-reared juveniles and preservation of environmental capacity and habitat. Therefore, mass production of marine finfish juveniles is being done for the intensive culture in net cage and for stocking coastal sea in Japan.

      Nearly 200 million juveniles are produced by ocean ranching centers (14 national, 49 prefectural, 21 city and town, 53 fishermen's association). The number of target fish is about 60 species (excluding salmon and trout). The main species produced are red sea bream, Pagrus major, flounder, Paralichthys olivaceus, puffer, Takifugu rubrapes, rockfish, Sebastes shlegeli, and mud dab, Limanda yokohamae. More than one million juveniles of these species are produced at one hatchery or ocean ranching center per one fry production season. About 70% of total production of juveniles consist of red sea bream and flounder. Red sea bream could be used to introduce mass larval rearing technology in Japan since its mass production is well developed. The focus of the present paper is the present status and short history of the development in larval rearing technology for red sea bream.
    • Conference paper

      Status of resource enhancement and sustainable aquaculture practices in Japan 

      K Okuzawa, T Takebe, N Hirai & K Ikuta - In MRR Romana-Eguia, FD Parado-Estepa, ND Salayo & MJH Lebata-Ramos (Eds.), Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production … International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA), 2015 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Contrary to the rapid increase in the world aquaculture production, fish production in Japan has been decreasing slightly due to the decreasing trend in seafood consumption of Japanese. Aquaculture production is approximately 20% in terms of yield, and 30% in terms of market value, of the country s total fisheries production. In Japan, about 80 species are targeted for release for sea ranching and resource enhancement purposes. The local governments (prefectures) are the main driving force in resource enhancement programs. Chum salmon, Oncorhynchus keta, and scallop Mizuhopecten yessoensisis are examples of successful resource enhancement in Japan. Japanese flounder, Paralichthys olivaceus, and red seabream, Pagrus major, represent intensely released fish species in Japan, and around 10% of the total catch of those species are estimated as released fish. The low price of products and increasing costs of production, such as costs of fuel and fish meal, are the major pressing issues in coastal fisheries and aquaculture in Japan. For aquaculture, the guarantee of food safety, minimization of environmental impact, and management of natural stock populations are highly necessary in order to achieve the sustainability of the industry. For resource enhancement, budget constraint is the major issue, and possible impact on natural stocks caused by released fish should also be considered. The Government of Japan (GOJ) is implementing some measures to rectify unstable business practices of aquaculture and to improve production techniques in aquaculture. For resource enhancement, the GOJ encourages cooperation among local governments (prefectures) for seed production and release of certain targeted species in order to reduce the cost and improve the efficiency of stock enhancement. In Japan, traditionally, the purpose for release was mainly sea ranching, namely harvesting all released animals. Nowadays, actual resource enhancement, i.e. the integrated release program including resource management and development of suitable nursery for released fish, is encouraged by the government. The evaluation and counter measures for the negative impact of stocked fish on genetic diversity of the wild population are also implemented. Recently, marked progress was achieved in seed production technologies of two important tropical fish species, namely coral trout, Plectropomus leopardus, and humphead wrasse, Cheilinus undulatus. These technologies are expected to contribute to the advancement of the aquaculture industry in the South East Asian region.