Now showing items 1-3 of 3

    • Article

      Anti-luminous Vibrio factors associated with the ‘green water’ grow-out culture of the tiger shrimp Penaeus monodon 

      The ability of the “green water” grow-out culture of the tiger shrimp Penaeus monodon to prevent outbreaks of Luminous Vibriosis was investigated by screening associated isolates of bacteria, fungi, phytoplankton and fish skin mucus for anti-luminous Vibrio metabolites. Among the 85 bacterial isolates tested, 63 (74%) caused +∼+++ inhibition of the Vibrio harveyi pathogen after 24–48 h co-cultivation. The variation in growth inhibition rates of +, ++, and +++ were demonstrated by 15 (18%), 13 (15%), and 28 (33%) isolates, respectively, 24 h after treatment. Eight bacterial isolates showed consistently sustained maximum inhibition of luminous Vibrio after 24 to 48 h exposure. The majority of these luminous Vibrio inhibiting bacterial isolates were obtained from tilapia mucus and gut. In tests with fungi, 4 of 20 (20%) yeast isolates showed intracellular metabolites inhibitory to luminous Vibrio. Among filamentous fungi, 5 of 45 (11%) isolates yielded intracellular metabolites while 3 of 41 (7%) isolates had extracellular metabolites inhibitory to luminous Vibrio. These fungal isolates were identified as Rhodotorula sp., Saccharomyces sp., Candida sp., Penicillium sp., mycelia sterilia, and two unidentified species. The microalgae, Chaetoceros calcitrans and Nitzchia sp., consistently demonstrated complete inhibition of luminous Vibrio from 24 h and 48 h post exposure, respectively, and during the 7-day experiment. Leptolyngbia sp. caused a 94–100% reduction of the luminous Vibrio population from 104 to 101 cfu/ml 24 h post exposure which was sustained throughout the 10-day observation period. In contrast, the inhibitory effects of Skeletonema costatum on luminous Vibrio was bacteriostatic throughout the 7-day exposure while Nannochlorum sp. did not significantly inhibit luminous Vibrio. The skin mucus of jewel tilapia, Tilapia hornorum, had no resident luminous bacteria and inhibited this bacterial pathogen in 6–48 h, which was proportionate to the 103 and 105 cfu/ml test concentrations of luminous Vibrio. This study provides a scientific explanation that the effectiveness of the “green water” culture of tiger shrimp (P. monodon) in preventing outbreaks of luminous Vibriosis among P. monodon juveniles in grow-out ponds can be attributed to the presence of anti-luminous Vibrio factors in the bacterial, fungal, phytoplankton microbiota and the skin mucus of tilapia associated with this novel technique of shrimp culture.
    • magazineArticle

      Live food: A lesser known essential 

      MB Surtida - SEAFDEC Asian Aquaculture, 2003 - Aquaculture Department, Southeast Asian Fisheries Development Center
      This article is a short discussion of the requirements for live food production in aquaculture and a brief presentation of the processes involved.
    • Conference paper

      Marine fish hatchery: developments and future trends 

      CL Marte & JD Toledo - In MRR Romana-Eguia, FD Parado-Estepa, ND Salayo & MJH Lebata-Ramos (Eds.), Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production … International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA), 2015 - Aquaculture Department, Southeast Asian Fisheries Development Center
      The basic procedures for producing marine fish fry in hatcheries developed for milkfish fry production nearly 3 decades ago are the basis of fry production systems for all other marine fish species that are now reared in hatcheries in the Philippines and other Southeast Asian countries. These include large-scale microalgae production in outdoor tanks, feeding of appropriate sized rotifer grown on microalgae such as Nannochlorum during the first feeding phase, and shifting to larger prey such as Artemia towards the latter stages of production.

      In recent years, the increasing demand for high-value species such as groupers, sea bass, red snapper, and pompano in both local and export markets has encouraged a number of hatcheries to produce fry to supply the requirements of fish cage farmers. Techniques are modified using information from research institutions and multi-national firms active in developing products and equipment to improve commercial production of these species. Larval feeds of appropriate sizes, forms and presentation for various larval stages incorporating essential nutrients, micronutrients, and feed stimulants are now available in the market. Diseases in marine fish hatcheries have become common occurrences such that various chemotherapeutants, vaccines, and immunostimulants are now available and increasingly being applied in fish hatcheries. Technological developments in hatchery systems, such as the use of recirculating systems, water pretreatment protocols (ozonation, mircrofiltration, UV light treatment) are also increasingly being adopted by commercial establishments.

      A critical link between fry production and production of marketable fish is fingerling/ juvenile production in nurseries. Fry are commonly grown in brackishwater fishponds to appropriate size for stocking in fish cages. Methods to improve growth through proper feeding and nutrition, eliminate or reduce disease occurrence and parasite infestation, reduce cannibalism in cannibalistic species such as sea bass, grouper and snappers are active areas of research. Nursery production is integrated with fry production in large commercial facilities but is also done by small-scale fish farmers who have access to fry either from the wild or hatcheries. Commercial hatcheries adopt fingerling production from well-studied species in developed countries. Smallscale farmers however still rely on zooplanktons collected from the wild such as copepods, Moina, mysids, and trash fish as feed. Production is dependent on availability of feed sources and susceptibility to pathogens and parasites that come with the feed. It can also be erratic since smallscale farms are vulnerable to changes in climate and weather conditions.

      Further technological advancement in marine fish hatcheries will increasingly be led by commercial establishments and industries developing equipment like photobioreactor for microalgae to produce algal paste, or methods to develop intensive systems for rotifer culture. Research institutions will however need to support the needs of the small-scale farmers and hatchery operators who may not be able to apply costly products from these companies by developing innovative simple techniques that can improve culture systems such as producing fry and fingerlings in mesocosm pond system, appropriate use of probiotics as water stabilizer, and production of zooplankton in ponds.