http://hdl.handle.net/10862/563 Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, 4-5 May 1993, Tigbauan, Iloilo, Philippines Thu, 09 Apr 2026 11:26:46 GMT 2026-04-09T11:26:46Z https://repository.seafdec.org.ph:443/bitstream/id/f9127b4b-e7c5-46ae-a9d1-63efcd787fa3/ http://hdl.handle.net/10862/563 http://hdl.handle.net/10862/579 Successful use of cryopreserved oyster trocophores as a live first feed larval marine fish and invertebrates Harvey, Brian J. Marte, Clarissa L.; Quinitio, Gerald F.; Emata, Arnil C. Trochophore-stage larvae of the Pacific oyster Crassostrea gigas were cryopreserved in bulk and stored in liquid nitrogen for periods up to two years before thawing and feeding to a variety of warmwater and coldwater larval marine fish, as well as to marine shrimps and other invertebrates. The commercial product ("TrochoFeed"), marketed in both pre-thawed and cryopreserved versions, has been used successfully in the early rearing of cultured species including red drum, snook, grouper, and black cod, as well as for numerous warmwater and coldwater aquarium display fish. This paper describes the nutritional profile of the cryopreserved trochopores and presents a summary of the available growth and mortality data. Mon, 01 Jan 1996 00:00:00 GMT http://hdl.handle.net/10862/579 1996-01-01T00:00:00Z http://hdl.handle.net/10862/577 Tilapia breeding and seed production for brackishwater culture in the Philippines Guerrero III, Rafael D.; Guerrero, Luzviminda A.; Cornejo, Rafael G. Marte, Clarissa L.; Quinitio, Gerald F.; Emata, Arnil C. Tilapias are important foodfishes in the Philippines second only to milkfish. While farming of tilapias in freshwater ponds and cages is already established, there is a need for wider application of the available technologies for brackishwater culture. This paper presents the tilapia species used for brackishwater farming and the commercial methods applied for their hatchery/nursery rearing. Mon, 01 Jan 1996 00:00:00 GMT http://hdl.handle.net/10862/577 1996-01-01T00:00:00Z http://hdl.handle.net/10862/571 Mass larval rearing technology of marine finfish in Japan Fukusho, Kunihiko Marte, Clarissa L.; Quinitio, Gerald F.; Emata, Arnil C. With economic development and increased demand for high price fish, industrial scale marine finfish culture in Japan was started in 1960-1965 for yellowtail Seriola quinqueradiata. Sustainable supply of wild juvenile and development of floating cage with synthetic fiber net have spurred the culture of nearly 30 species and total production in 1991 is 265 x 103 metric tons (nearly 25% of total aquaculture production). Although salmon ranching had been started in 1888, a national project of ocean ranching was only initiated in 1963 with the present target of 26 species of marine finfish. Ocean ranching aims to increase fisheries resources in coastal sea by stocking hatchery-reared juveniles and preservation of environmental capacity and habitat. Therefore, mass production of marine finfish juveniles is being done for the intensive culture in net cage and for stocking coastal sea in Japan. Nearly 200 million juveniles are produced by ocean ranching centers (14 national, 49 prefectural, 21 city and town, 53 fishermen's association). The number of target fish is about 60 species (excluding salmon and trout). The main species produced are red sea bream, Pagrus major, flounder, Paralichthys olivaceus, puffer, Takifugu rubrapes, rockfish, Sebastes shlegeli, and mud dab, Limanda yokohamae. More than one million juveniles of these species are produced at one hatchery or ocean ranching center per one fry production season. About 70% of total production of juveniles consist of red sea bream and flounder. Red sea bream could be used to introduce mass larval rearing technology in Japan since its mass production is well developed. The focus of the present paper is the present status and short history of the development in larval rearing technology for red sea bream. Mon, 01 Jan 1996 00:00:00 GMT http://hdl.handle.net/10862/571 1996-01-01T00:00:00Z http://hdl.handle.net/10862/566 Influence of LHRHa and methyltestosterone on milt production of sea bass Lates calcarifer (Bloch) Hilomen-Garcia, G. V.; Baldevarona, R. B.; Lacanilao, F. J. Marte, Clarissa L.; Quinitio, Gerald F.; Emata, Arnil C. Milt volume, sperm density, and number of spermatozoa were determined to quantify milt production of mature sea bass after a single injection of LHRHa [(D-Ala6,Pro9-N-ethylamiide)LHRH] in saline solution and 17α-methyltestosterone in corn oil (MT). Two measures of sperm density, sperm count and spermatocrit, were highly correlated (r=0.85). Compared with control, milt volume and the number of spermatozoa collected increased but sperm count decreased (24% at 24 h) after a LHRHa (20 µg/kg body weight treatment, suggesting a stimulation of spermatozoa production and not merely milt dilution. Further milt dilution (44%) was induced by 80 µg/kg LHRHa (LHRHa80) at 12 h post-treatment but not by 200 µg/kg MT (MT200) alone. A milt dilution of only 27% at 12 h after simultaneous injections of LHRHa80 and MT200 may indicate some inhibitory effect of MT on the efficiency of LHRHa. These results demonstrate that the stimulation of milt production by LHRHa involves testicular hydration resulting in milt dilution. Abstract only. Mon, 01 Jan 1996 00:00:00 GMT http://hdl.handle.net/10862/566 1996-01-01T00:00:00Z