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Prevalence of economically-important viral diseases in wild shrimp (Penaeus monodon) in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Prevalences of white spot syndrome virus (WSSV) and monodon baculovirus (MBV) were determined using polymerase chain reaction (PCR) as the detection method in wild black tiger shrimp (Penaeus monodon) collected from seven ...
Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Several runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot ...
Studies on the experimental transmission model of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Series of experiments on the transmission of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon were undertaken. Feeding of MBV-infected carcasses to experimental shrimps resulted to 100% ...
Establishment of preventive measures against viral nervous necrosis (VNN) in finfish hatcheries
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
The establishment of preventive measures against viral nervous necrosis (VNN) at the finfish hatcheries of SEAFDEC Aquaculture Department in Iloilo, Philippines, was instituted primarily to minimize the impact of the viral ...
Development of monoclonal antibody (MAb) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Antibody-based detection methods are relatively simple and rapid, aside from being specific and sensitive. Thus, there is a necessity to develop such techniques for early and rapid diagnosis of viral pathogens such as MBV ...
Standardization of PCR techniques as the detection method for koi herpesvirus (KHV) infection in koi and common carp
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Several runs have been made using the two published PCR protocols in the detection of koi herpesvirus (KHV). The primer pairs designed by individual authors based from the sequence of a cloned fragment of KHV genome ...
Series: JIRCAS Working Report;No. 35
Development of control methods of factors suppressing sustainable production of aquaculture species: Isolation of a piscine nodavirus from hatchery-reared sea bass Lates calcarifer in the Philippines
(Japan International Center for Agricultural Sciences, 2003)
The etiological study on mass mortality of larval sea bass Lates calcarifer cultured in the Philippines, demonstrated that the mortality was caused by viral nervous necrosis (VNN). Affected sea bass exhibited abnormal ...
Series: JIRCAS Working Report;No. 35
Development for control methods for factors suppressing sustainable production of aquaculture species: Experimental transmission of piscine nodavirus-induced viral nervous necrosis to the orange-spotted grouper Epinephelus coioides
(Japan International Center for Agricultural Sciences, 2003)
Viral nervous necrosis (VNN) of larval orange-spotted grouper Epinephelus coioides has been recorded recently in the Philippines. The present study describes the pathogenicity of a piscine nodavirus (betanodavirus), the ...