Now showing items 1-4 of 4

    • Article

      Feeding selectivity of the seahorse, Hippocampus kuda (Bleeker), juveniles under laboratory conditions 

      FT Celino, GV Hilomen-Garcia & AGC del Norte-Campos - Aquaculture Research, 2012 - Blackwell Publishing Ltd
      This study examined the feeding selectivity of Hippocampus kuda juveniles under captive conditions and evaluates different food organisms that could be used to improve hatchery-rearing of this species. Newly born H. kuda were reared for 10 days in 60-L capacity tanks and fed rotifers (Brachionus rotundiformis), zooplankton (mostly Pseudodiaptomus annandalei and Acartia tsuensis) alone or both food sources. The size and amount of food ingested increased as seahorses grew. Selective feeding of seahorses appeared to change as they develop, preferring copepod adults over nauplii and rotifers. A. tsuensis was highly selected by juveniles over P. annandalei. Specific growth rate in terms of body weight (SGR-BW, 15% day–1) was the highest and mortality rate (9% at day 10) the lowest in seahorses fed a mixed food sources. Slowest growth rate (0.3% day–1) and highest mortality rate (60% at day 7) were observed in seahorses fed rotifers alone. These results indicate that copepods are suitable food for seahorse juveniles, but a mixture of food organisms in the rearing tank environment enhances survivorship and growth of H. kuda, thus potentially providing a source of cultured rather than wild specimens for characterizing the life history of this threatened species.
    • Article

      Population dynamics of the calanoid copepod, Acartia tsuensis in a brackish-water pond in the Philippines 

      MSN Golez, A Ohno, JD Toledo, Y Tanaka & T Ishimaru - Fisheries Science, 2002 - The Japanese Society of Fisheries Science
      The occurrence pattern and population dynamics of Acartia tsuensis were investigated in a brackish-water pond in Panay Island in central Philippines by implementing both bi-monthly and daily sampling schemes. A. tsuensis occurred in the pond during the dry season (November-April) when the salinity of the water is in the range of 14 ~ 40 ppt but was completely absent at lower salinities. An almost constant rate of development from the nauplius 2 through to copepodite 5 stages of A. tsuensis was observed both in the pond and in the laboratory. The generation time ranged from 5.9~11.3 days. Fecundity had a positive linear corelation with chlorophyll a. Salinity and chlorophyll a affect the stage duration, mortality, and fecundity of A. tsuensis in the pond.
    • Article

      Preliminary investigation of feeding performance of larvae of early red-spotted grouper, Epinephelus coioides, reared with mixed zooplankton 

      M Doi, JD Toledo, MSN Golez, M de los Santos & A Ohno - Hydrobiologia, 1997 - Springer Verlag
      Larvae of red-spotted grouper, Epinephelus coioides, were reared in outdoor tanks with nauplii of copepods (mainly Pseudodiaptomus annandalei and Acartia tsuensis) and/or rotifers, Brachionus rotundiformis. Grouper larvae successfully started feeding on early stage nauplii even though their abundance was as low as approximately 100 individuals l-1 and showed better survival and growth thereafter compared to those fed with rotifers only. Incidence of feeding reached 100% on day 4 when nauplii were available and only on day 9 when rotifers were given alone. Larvae seemed to be poor feeders at the onset of feeding, attempting to capture any food organisms in the tank water. Selective feeding ability of larvae started from day 4 and the larvae then preferred to feed on medium- and large-size nauplii rather than on rotifers as they grew. Larvae appeared to have a better chance at surviving in the presence of early stage nauplii, which were probably caught more easily than rotifers.
    • Article

      Use of copepod nauplii during early feeding stage of grouper Epinephelus coioides 

      JD Toledo, MS Golez, M Doi & A Ohno - Fisheries Science, 1999 - Japanese Society of Fisheries Science
      Newly-hatched Epinephelus coioides larvae were stocked in five 5-ton tanks at an initial density of 25, 000 ind/tank. Copepod nauplii were propagated in four of these tanks by inoculating various densities (20 to 80 ind/l) of mixed copepodids of Acartia tsuensis, Pseudodiaptomus spp., and Oithona sp. three days before stocking larvae. Rotifers were added in these tanks on Day 7 at an initial density of 5, 000 ind/l. Larvae in the remaining tank were fed rotifers (only) starting Day 2 at 5, 000 ind/l. The feeding incidence, gut content, growth, and survival of larvae were better in tanks with higher density of copepodids (60-80 ind/l). These indices were lowest in larvae given rotifers only. Total n-3 HUFA of copepods was 2 to 3 times higher than rotifers. High percentages of 22:6n-3 (DHA) were detected in the fatty acid composition of Pseudodiaptomus (13%) and Acartia (24%) with DHA/EPA (20:5n-3) values of 1.4 and 2.6, respectively. By providing nauplii of copepods at the early feeding stage, an average survival of 3.4% at harvest (Day 36) was obtained in a pilot scale grouper seed production trial in three 10-ton tanks.