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Journal: Journal of Applied Phycology

Seasonal growth, carrageenan properties, and resistance to disease and epiphytic pests between Kappaphycus alvarezii (Rhodophyta) var. tambalang (brown) tissue-cultured and farm-sourced seaweeds 

Faisan, Jr., Joseph ORCID; Samson, Edcel Jed D.; Sollesta-Pitogo, Hananiah; Dayrit, Rheniel; Balinas, Vicente T.; de la Peña, Leobert D. (Springer, 2024-01-08)
Growth performance (specific growth rate [SGR] and biomass yield), carrageenan properties (yield, viscosity, and gel strength), and disease and pest incidence were compared between Kappaphycus alvarezii var. tambalang ...
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Journal: Research in Veterinary Science

In vitro and in vivo evaluation of the efficacies of commercial probiotics and disinfectant against acute hepatopancreatic necrosis disease and luminescent vibriosis in Litopenaeus vannamei 

de la Peña, Leobert D.; Moquera, Germin L.; Amar, Edgar ORCID; Castellano, Jose Louis A.; Cabillon, Nikko Alvin R.; Arboleda, Joey I.; Nava, Joseph Keith Paulo ORCID; Zamora, Rodolfo V. Jr.; De Schryver, Peter (Elsevier, 2024-05)
The bioactivities of two commercially available probiotics and one chemical disinfectant were tested against strains of Vibrio parahaemolyticus (VP<sub>AHPND</sub>) and V. harveyi. This study aimed to determine shrimp pathogenic Vibrios' in vitro and in vivo sensitivities to commercial probiotics and a chemical disinfectant. The probiotics and disinfectant were tested first in vitro, followed by the in vivo trials. Results showed that upon administration of probiotics either through diet or adding into the tank water, the survivability of shrimp was increased during challenge with VP<sub>AHPND</sub> and V. harveyi. Also, the disinfectant was tested against the same pathogens and showed positive bactericidal effects at 2500 ppm and 5000 ppm. The present findings suggest that adding probiotics to the rearing water or the shrimp feeds effectively prevents infection by lowering the load of pathogenic bacteria. In comparison, the effectiveness of the disinfectant (PUR) depends on its appropriate concentration and timing of application. It is not only limited to rearing water but is also applicable for decontaminating pond liners, tanks, and other paraphernalia....
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Journal: Aquacultural Engineering

The impact of indoor biofloc-based system on water quality, growth, and disease resistance of black tiger shrimp 

Estante-Superio, Erish ORCID; de la Peña, Leobert D.; Geanga, Therese Marie M.; Castellano, Jose Louis A.; Cordero, Christian P.; Berlin, Sharmen C.; Lazado, Carlo C. (Elsevier, 2025-10)
The present study investigates the efficiency of an indoor biofloc-based system for the intensive culture of black tiger shrimp (Penaeus monodon). Water quality, growth performance, and disease resistance of black tiger shrimp (0.35 ± 0.07 g) were evaluated after 90 days of rearing in a zero-water exchange system. Shrimp were stocked at 180 individuals/m<sup>3</sup> in nine 5-ton concrete tanks under three treatments: biofloc with molasses (M), biofloc with wheat flour (WF), and a control group reared in a conventional clear water (CW) flow-through system. The WF treatment resulted in significantly higher final weight and improved FCR compared to CW (p &lt; 0.05). Survival was also significantly higher in both biofloc-based treatments despite elevated concentrations of total ammonia nitrogen, nitrite-nitrogen, and nitrate-nitrogen levels in the WF group (p &lt; 0.05). Additionally, Kaplan–Meier survival analysis indicated that shrimp reared and challenged in the biofloc system exhibited better resistance to Vibrio parahaemolyticus (VP<sub>AHPND</sub>1212), as evidenced by higher survival rates. Although total hemocyte count, prophenoloxidase, and respiratory burst activities were not significantly different from the control group (p &gt; 0.05), they were consistently elevated in the biofloc-based treatments. Overall, biofloc-based treatments, specifically WF, proved effective in biofloc formation, contributing to improved water quality, enhanced immune response and disease resistance, and superior growth performance in P. monodon. This study highlights the potential of indoor biofloc systems as a sustainable strategy for intensive P. monodon culture in indoor biofloc tanks....
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Journal: Regional Studies in Marine Science

First report on the use of various fishing gears and transport schemes for the captive breeding of shortfin scad (Decapterus macrosoma) as a potential species for aquaculture 

Legaspi, Ma. Irene ORCID; Ito, Sayaka ORCID; de la Peña, Leobert D.; Silorio, Jernet Zyca (Elsevier, 2026-01)
This study establishes the first protocols for the live capture and transport of shortfin scad (<i>Decapterus macrosoma</i>), a potential new species for aquaculture. Wild fish were collected in the central Philippines ...


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de la Peña, Leobert D. (4)
Castellano, Jose Louis A. (2)Amar, Edgar (1)Arboleda, Joey I. (1)Balinas, Vicente T. (1)... View MoreSubjectPhilippines (3)aquaculture (2)disease resistance (2)diseases (2)growth (2)... View MoreTaxonomic termDecapterus macrosoma (1)Kappaphycus alvarezii (1)Litopenaeus vannamei (1)Penaeus monodon (1)Penaeus vannamei (1)... View MoreDate Issued2024 (2)2025 (1)2026 (1)TypeArticle (4)SDG contribution
SDG 14 - Life below water (4)
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