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  • 03 SEAFDEC/AQD External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
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  • 03 SEAFDEC/AQD External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
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Now showing items 31-40 of 41

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Journal: Preventive Veterinary Medicine

Enhancing disease monitoring in shrimp through a geographical information system (GIS) application 

Lavilla-Pitogo, C. R.; de la Peña, Leobert D.; Tendencia, Eleonor ORCID (Elsevier, 2007)
SEAFDEC Aquaculture Department (AQD) pioneered fish disease work in the Philippines and developed diagnostic tools through research. Its Diagnostic Service Laboratory was established in the late 1970s to serve the budding ...
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Journal: Bulletin of the European Association of Fish Pathologists

Koi herpesvirus-associated mortalities in quarantined koi carp in the Philippines 

Somga, J. R.; de la Peña, Leobert D.; Sombito, C. D.; Paner, Milagros G.; Suarnaba, Vonnie S.; Capulos, Geimbo C.; Santa Maria, P. I.; Po, G. L. (European Association of Fish Pathologists, 2010)
Illegally imported koi carp were confiscated at the Ninoy Aquino International Airport (NAIA), Manila, Philippines by the Fisheries Quarantine and Inspection Service Officers of the Bureau of Fisheries and Aquatic Resources ...
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Journal: Aquaculture

Prevalence of monodon baculovirus (MBV) in wild shrimp Penaeus monodon in the Philippines 

de la Peña, Leobert D.; Lavilla-Pitogo, Celia R.; Villar, Corina Belle R.; Paner, Milagros G.; Capulos, Geimbo C. (Elsevier, 2008)
Prevalence of monodon baculovirus (MBV) was determined using polymerase chain reaction (PCR) on deoxyribonucleic acid (DNA) extracts from the hepatopancreas of the wild black tiger shrimp, Penaeus monodon collected from 7 ...
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Journal: Research in Veterinary Science

In vitro and in vivo evaluation of the efficacies of commercial probiotics and disinfectant against acute hepatopancreatic necrosis disease and luminescent vibriosis in Litopenaeus vannamei 

de la Peña, Leobert D.; Moquera, Germin L.; Amar, Edgar ORCID; Castellano, Jose Louis A.; Cabillon, Nikko Alvin R.; Arboleda, Joey I.; Nava, Joseph Keith Paulo ORCID; Zamora, Rodolfo V. Jr.; De Schryver, Peter (Elsevier, 2024-05)
The bioactivities of two commercially available probiotics and one chemical disinfectant were tested against strains of Vibrio parahaemolyticus (VP<sub>AHPND</sub>) and V. harveyi. This study aimed to determine shrimp pathogenic Vibrios' in vitro and in vivo sensitivities to commercial probiotics and a chemical disinfectant. The probiotics and disinfectant were tested first in vitro, followed by the in vivo trials. Results showed that upon administration of probiotics either through diet or adding into the tank water, the survivability of shrimp was increased during challenge with VP<sub>AHPND</sub> and V. harveyi. Also, the disinfectant was tested against the same pathogens and showed positive bactericidal effects at 2500 ppm and 5000 ppm. The present findings suggest that adding probiotics to the rearing water or the shrimp feeds effectively prevents infection by lowering the load of pathogenic bacteria. In comparison, the effectiveness of the disinfectant (PUR) depends on its appropriate concentration and timing of application. It is not only limited to rearing water but is also applicable for decontaminating pond liners, tanks, and other paraphernalia....
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Journal: Bulletin of the European Association of Fish Pathologists

Susceptibility of different weight ranges of Epinephelus coioides to piscine nodavirus 

de la Peña, Leobert D.; Suarnaba, Vonnie S.; Villacastin, Anne Jinky B.; Cabillon, Nikko Alvin R.; Catedral, Demy D.; Faisan, Jr., Joseph ORCID (European Association of Fish Pathologists, 2017)
The susceptibility of different weight ranges of orange-spotted grouper (Epinephelus coioides) to piscine nodavirus was studied by experimental infection. Size ranges of 1-2 g, 6-8 g, 10-13 g, 30-60 g, and 90-120 g were ...
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Journal: Asian Fisheries Science

Evaluation of various leguminous seeds as protein sources for milkfish, Chanos chanos Forsskal, juveniles 

de la Peña, Leobert D.; Chiu, Yvonne N.; Ganchero, Fe (Asian Fisheries Society, 1987)
The nutritive value of four leguminous plant seeds: pigeonpea (Cajanus cajan), mungo (Phaseolus radiatus), kidneybean (Phaseolus vulgaris) and soybean (Glycine max), were evaluated as protein sources for milkfish (Chanos ...
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Journal: Microbiology Resource Announcements

Complete genome sequence of Vibrio parahaemolyticus strain PH1273, isolated from aquacultured shrimp in the Philippines 

Salazar, John Raymond B.; Santos, Mary Nia; Palaad, Jeremy L.; Yen, Hsi-Ching ORCID; Castellano, Jose Louis A.; de la Peña, Leobert D.; Amar, Edgar ORCID; Lai, Erh-Min ORCID; Kuo, Chih-Horng ORCID (American Society for Microbiology, 2023-10-19)
We announce the complete genome sequence of Vibrio parahaemolyticus strain PH1273. This strain was collected from a Penaeus vannamei pond in the Philippines in 2015. Genome analysis revealed that it lacks the gene pirAB ...
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Journal: Bulletin of the European Association of Fish Pathologists

Sandfish (Holothuria scabra) as potential reservoir of white spot syndrome virus (WSSV) when co-cultured with black tiger prawn (Penaeus monodon) 

de la Peña, Leobert D.; Caber, Dieyna B.; Villacastin, Anne Jinky B.; Cabillon, Nikko Alvin R.; Arboleda, Joey I.; Castellano, Jose Louis A.; Nava, Joseph Keith Paulo ORCID; Watanabe, Satoshi (European Association of Fish Pathologists, 2024-03-08)
Since the first occurrence of White Spot Disease (WSD) in 1992, it is still listed as one of the crustacean diseases by the World Organisation for Animal Health in 2022. Horizontal transmission in co-culture systems is one ...
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Journal: Aquacultural Engineering

The impact of indoor biofloc-based system on water quality, growth, and disease resistance of black tiger shrimp 

Estante-Superio, Erish ORCID; de la Peña, Leobert D.; Geanga, Therese Marie M.; Castellano, Jose Louis A.; Cordero, Christian P.; Berlin, Sharmen C.; Lazado, Carlo C. (Elsevier, 2025-10)
The present study investigates the efficiency of an indoor biofloc-based system for the intensive culture of black tiger shrimp (Penaeus monodon). Water quality, growth performance, and disease resistance of black tiger shrimp (0.35 ± 0.07 g) were evaluated after 90 days of rearing in a zero-water exchange system. Shrimp were stocked at 180 individuals/m<sup>3</sup> in nine 5-ton concrete tanks under three treatments: biofloc with molasses (M), biofloc with wheat flour (WF), and a control group reared in a conventional clear water (CW) flow-through system. The WF treatment resulted in significantly higher final weight and improved FCR compared to CW (p &lt; 0.05). Survival was also significantly higher in both biofloc-based treatments despite elevated concentrations of total ammonia nitrogen, nitrite-nitrogen, and nitrate-nitrogen levels in the WF group (p &lt; 0.05). Additionally, Kaplan–Meier survival analysis indicated that shrimp reared and challenged in the biofloc system exhibited better resistance to Vibrio parahaemolyticus (VP<sub>AHPND</sub>1212), as evidenced by higher survival rates. Although total hemocyte count, prophenoloxidase, and respiratory burst activities were not significantly different from the control group (p &gt; 0.05), they were consistently elevated in the biofloc-based treatments. Overall, biofloc-based treatments, specifically WF, proved effective in biofloc formation, contributing to improved water quality, enhanced immune response and disease resistance, and superior growth performance in P. monodon. This study highlights the potential of indoor biofloc systems as a sustainable strategy for intensive P. monodon culture in indoor biofloc tanks....
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Journal: Regional Studies in Marine Science

First report on the use of various fishing gears and transport schemes for the captive breeding of shortfin scad (Decapterus macrosoma) as a potential species for aquaculture 

Legaspi, Ma. Irene ORCID; Ito, Sayaka ORCID; de la Peña, Leobert D.; Silorio, Jernet Zyca (Elsevier, 2026-01)
This study establishes the first protocols for the live capture and transport of shortfin scad (<i>Decapterus macrosoma</i>), a potential new species for aquaculture. Wild fish were collected in the central Philippines ...

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de la Peña, Leobert D. (41)
Amar, Edgar (6)Faisan, Jr., Joseph (6)Lavilla-Pitogo, Celia R. (6)Cabillon, Nikko Alvin R. (5)... View MoreSubjectPhilippines (24)Penaeus monodon (14)aquaculture (10)polymerase chain reaction (9)shrimp culture (8)... View MoreTaxonomic termPenaeus monodon (14)Penaeus vannamei (5)Vibrio harveyi (5)Lates calcarifer (4)Vibrio parahaemolyticus (3)... View MoreDate Issued2020 - 2026 (9)2010 - 2019 (6)2000 - 2009 (19)1990 - 1999 (6)1987 - 1989 (1)TypeArticle (38)Preprint (1)SDG contributionSDG 14 - Life below water (5)Has File(s)No (40)Yes (1)

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