Now showing items 1-7 of 7

    • Conference paper

      Changes in lipid and fatty acid content during early larval development of milkfish (Chanos chanos): influence of broodstock diet 

      CL Marte, IG Borlongan & AC Emata - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development Center
      The influence of amount and type of lipid given to milkfish broodstock by developing larvae was investigated by feeding broodstock commercial diets that differed in lipid content and composition. The two commercial feeds used had the following proximate composition: RFP - 28.16% crude protein, 2.40% crude fat, 7.58% crude fiber, 53.94% N-free extract; RCP - 43.28% crude protein, 4.58% crude fat, 6.18% crude fiber, 37.0% N-free extract.

      The lipid content and fatty acid composition of spawned milkfish eggs reflected that of the broodstock feed. Percent lipid in egg from broodstock fed RFP and RCP dropped by 22.5% and 26.9% in newly-hatched larvae and by 53.0% and 65.0% in day 2 larvae (>90% yolk resorbed), respectively. Decreases in total PUFA and increase in monoenoic fatty acids during yolk resorption indicate that milkfish as in other marine fishes utilize PUFA during early larval development. While differences in rate of utilization of individual n-3 and n-6 FA in two groups of larvae seem to be influenced by levels of the fatty acids in eggs, the influence of other nutrients on fatty acid utilization need to be investigated.
    • Conference paper

      Flounder metamorphosis: its regulation by various hormones 

      EG de Jesus, T Hirano & Y Inui - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development Center
      The elongation and shortening of the flounder fin rays during its metamorphosis may be parallel to the appearance and resorption of the tadpole tail during amphibian metamorphosis. The dorsal fin rays of the Japanese flounder (Paralichthys olivaceus) elongate during the time when thyroid hormone levels are low and are resorbed during climax of metamorphosis when thyroid levels are elevated. Using an in vitro system for culture of isolated flounder fin rays, we examined how various hormones affect the process of resorption. Both T4 and T3 directly stimulated fin ray shortening, T3 being more potent than T4. Other hormones did not directly affect the resorption process but modified the tissue's response to thyroid hormones. Cortisol enhanced the stimulatory effects of both T4 and T3. On the other hand, estradiol and testosterone were inhibitory. Ovine prolactin (oPRL) also diminished the effect of T3 while ovine growth hormone (oGH) was without effect. Similar observations were observed from the in vivo studies.

      Thyroid hormone levels, especially T4, were low during premetamorphosis, increased during prometamorphosis, to peak levels during metamorphic climax, and declined in the juveniles. The changes in whole body concentrations of Cortisol paralleled the changes in thyroid hormone concentrations. On the other hand, whole body estradiol and testosterone concentrations did not show significant change and remained low throughout the larval period. The expression of GH and PRLmRNA, as assessed by in situ hybridization using cDNA for flounder PRL and GH also increased during the metamorphosis. However, the increase in the expression of GH and PRL genes was observed later than the increases in tissue levels of thyroid hormones and Cortisol. At late climax, the flounder larvae have undergone considerable transformation, presumably triggered by both hormones, hence the increase in PRL could hardly be antimetamorphic but may have other physiological implications.
    • Conference paper

      Larviculture of milkfish (Chanos chanos) in outdoor tanks 

      MN Duray - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development Center
      In the past, larviculture of milkfish depended entirely on the use of rotifers and brine shrimp nauplii and rearing trials were done under roofed facilities. Since the dietary value of live food varies according to culture and feeding conditions, rotifers were enriched with SELCO, a lipid emulsion containing high levels of highly unsaturated fatty acids (HUFA) prior to feeding the larvae. Alternatively, a microbound larval feed (Nosan R-1) was given as a supplement to rotifers during the first two weeks of culture. Larval growth was enhanced and survival was significantly improved when rotifers were enriched or supplemented with these diets. All rearing trials were conducted in 5-10 tons concrete circular/rectangular outdoor tanks.

      Verification runs on the use of HUFA-enriched rotifers to milkfish larvae were tried in two nearby private hatcheries. Results from mis collaborative work are presented.
    • Conference paper

      Mass larval rearing technology of marine finfish in Japan 

      K Fukusho - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development Center
      With economic development and increased demand for high price fish, industrial scale marine finfish culture in Japan was started in 1960-1965 for yellowtail Seriola quinqueradiata. Sustainable supply of wild juvenile and development of floating cage with synthetic fiber net have spurred the culture of nearly 30 species and total production in 1991 is 265 x 103 metric tons (nearly 25% of total aquaculture production). Although salmon ranching had been started in 1888, a national project of ocean ranching was only initiated in 1963 with the present target of 26 species of marine finfish. Ocean ranching aims to increase fisheries resources in coastal sea by stocking hatchery-reared juveniles and preservation of environmental capacity and habitat. Therefore, mass production of marine finfish juveniles is being done for the intensive culture in net cage and for stocking coastal sea in Japan.

      Nearly 200 million juveniles are produced by ocean ranching centers (14 national, 49 prefectural, 21 city and town, 53 fishermen's association). The number of target fish is about 60 species (excluding salmon and trout). The main species produced are red sea bream, Pagrus major, flounder, Paralichthys olivaceus, puffer, Takifugu rubrapes, rockfish, Sebastes shlegeli, and mud dab, Limanda yokohamae. More than one million juveniles of these species are produced at one hatchery or ocean ranching center per one fry production season. About 70% of total production of juveniles consist of red sea bream and flounder. Red sea bream could be used to introduce mass larval rearing technology in Japan since its mass production is well developed. The focus of the present paper is the present status and short history of the development in larval rearing technology for red sea bream.
    • Conference paper

      Natural spawning of four Epinephelus species in the laboratory 

      MNR Alava, MLL Dolar & JA Luchavez - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Natural spawnings of four Epinephelus species reared in the laboratory were observed from 1987 to 1992. These species are: E. summana, E. caeruleopunctatus, E. macrospilus and E. fuscoguttatus. Spawning was serial, usually occurring at night, on or 1-6 days after the new moon. Egg characteristics of these four species were compared. Fertilized egg and early larval development of E. summana and E. fuscoguttatus are discussed.
    • Conference paper

      A review of grouper (Epinephelus suillus) fry production research in Malaysia 

      HM Ali - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Research on grouper (Epinephelus suillus) fry production in captivity has been carried out in Malaysia since 1986 at Tanjung Demong Marine Finfish Production and Research Centre (TDMFPRC) but the breakthrough was only achieved four years later in 1990. Eggs were obtained through natural and induced spawning in tanks. Natural spawning of grouper in captivity seldom occurred and was unpredictable. However induced spawnings were successfully carried out by injecting human chorionic gonadotropin (hCG) intramuscularly at a dose of 500-1000 IU/kg fish.

      The results from several trials on larval rearing conducted since 1989 until recently showed that larvae obtained from natural spawnings survived longer with some reaching the juvenile stage. The highest recorded survival rate of 43 days posthatch (32.5 mm total length) was 12.1% at 28-32 °C water temperature. On the other hand, 100% mortality usually occurred in larvae obtained from induced spawning 7 days after hatching.

      The major constraints of grouper fry production in Malaysia are lack of male spawners, inconsistent and unpredictable natural spawning, small quantity of eggs released every spawning day, poor fertilization and hatching rate, weak hatchlings, and high mortality rate at the early stages of larval development. The latter is probably due mainly to problems on initial feeding.
    • Conference paper

      Review of SEAFDEC/AQD finfish seed production research 

      GF Quinitio & MN Duray - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Research on seed production of several foodfishes has been a continuing activity of SEAFDEC/AQD since 1976. Fry and juvenile production methods of these fish commodities are in various stages of advancement. For instance, advances in the development of hatchery rearing, particularly feeding and water management schemes, have made mass production of milkfish (Chanos chanos) seed a reality, resulting further in the application of the technology in commercial hatcheries. Recent studies now focus on assessing the quality of hatchery seed stocks of milkfish vis-a-vis wild seed during nursery and grow-out culture. Likewise, sea bass (Lates calcarifer) seed production has undergone significant improvements since the technology was introduced in the Philippines in 1982. Fatty acid-enrichment of a zooplankton diet can enhance growth and survival of sea bass fry, although other cheaper alternatives and early weaning to formulated diet preparations are currently being tested. Hatchery fry production of grouper (Epinephelus salmoides and E. suillus syn. E. coioides) and snapper is in its infancy, but trials complemented by research on their larval feeding habits and requirements are underway to establish reliable methods of rearing larvae of these species. Although fairly well-established, seed production of rabbitfish (Siganus guttatus) requires further improvement in determining an appropriate zooplankton diet to ensure adequate growth and survival of larvae. Hatchery fry production of tilapia (Oreochromis sp.), carps (Aristichthys nobilis, Hypothalmichthys molitrix) and, to a certain extent, catfish (Clarias macrocephalus) can already be categorized as a flourishing industry in some parts of the Philippines. Nonetheless, SEAFDEC/AQD continues to conduct research on these freshwater species, with particular emphasis on nutrition and feed development during the nursery production phase. Together, results of past and on-going research studies ensure that seed supply of these important foodfishes become adequate and sustainable for the grow-out.