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Studies on the experimental transmission model of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Series of experiments on the transmission of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon were undertaken. Feeding of MBV-infected carcasses to experimental shrimps resulted to 100% ...
Development of immunological indices for monitoring health status in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Some hemolymph parameters were determined as non-invasive early-warning indicators of health status in black tiger shrimp (Penaeus monodon). These included total hemocyte count (THC), respiratory burst activity, phenoloxidase ...
Prevalence of economically-important viral diseases in wild shrimp (Penaeus monodon) in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Prevalences of white spot syndrome virus (WSSV) and monodon baculovirus (MBV) were determined using polymerase chain reaction (PCR) as the detection method in wild black tiger shrimp (Penaeus monodon) collected from seven ...
Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Several runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot ...
Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) remain as two of the serious pathogens that infect black tiger shrimp (Penaeus monodon) in the Philippines. It is, therefore, necessary to prevent the spread ...
Use of bacteria as biological control agent against microbial diseases in shrimp (Penaeus monodon) and crab (Scylla serrata) hatcheries: Part I. Isolation and selection of bacteria for biological control in crustacean hatcheries
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
A study aimed at obtaining a biological control agent against bacterial diseases of hatchery-reared shrimps and crabs was conducted. Bacteria were isolated from crustacean rearing environments where luminescent vibriosis ...
Development of monoclonal antibody (MAb) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Antibody-based detection methods are relatively simple and rapid, aside from being specific and sensitive. Thus, there is a necessity to develop such techniques for early and rapid diagnosis of viral pathogens such as MBV ...
Chlorine disinfection against hepatopancreatic parvovirus (HPV) infection in shrimp (Penaeus monodon) larvae
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Experiments were undertaken to determine the efficacy of chlorine as a disinfectant in inhibiting HPV infection among P. monodon postlarvae (PLs). Based on histopathological examination, 50 ppm and 200 ppm active chlorine ...
Standardization of PCR techniques as the detection method for koi herpesvirus (KHV) infection in koi and common carp
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Several runs have been made using the two published PCR protocols in the detection of koi herpesvirus (KHV). The primer pairs designed by individual authors based from the sequence of a cloned fragment of KHV genome ...
Establishment of preventive measures against viral nervous necrosis (VNN) in finfish hatcheries
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
The establishment of preventive measures against viral nervous necrosis (VNN) at the finfish hatcheries of SEAFDEC Aquaculture Department in Iloilo, Philippines, was instituted primarily to minimize the impact of the viral ...