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Studies on the experimental transmission model of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Series of experiments on the transmission of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon were undertaken. Feeding of MBV-infected carcasses to experimental shrimps resulted to 100% ...
Development of immunological indices for monitoring health status in shrimp (Penaeus monodon)
Amar, Edgar C. 
; ; (Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
; ; (Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Some hemolymph parameters were determined as non-invasive early-warning indicators of health status in black tiger shrimp (Penaeus monodon). These included total hemocyte count (THC), respiratory burst activity, phenoloxidase ...
Prevalence of economically-important viral diseases in wild shrimp (Penaeus monodon) in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Prevalences of white spot syndrome virus (WSSV) and monodon baculovirus (MBV) were determined using polymerase chain reaction (PCR) as the detection method in wild black tiger shrimp (Penaeus monodon) collected from seven ...
Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Several runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot ...
Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia: General information
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Research is the main component of the Regional Fish Disease Project supported by the Government of Japan (GOJ) Trust Fund. When the project started in 2000, research was undertaken by scientists of the SEAFDEC Aquaculture ...
Development of shrimp (Penaeus monodon) cell culture in vitro
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Primary shrimp cell cultures were successfully developed from lymphoid organ of black tiger shrimp (Penaeus monodon) in double-strength Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum, 1% glucose, 15% ...
Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) remain as two of the serious pathogens that infect black tiger shrimp (Penaeus monodon) in the Philippines. It is, therefore, necessary to prevent the spread ...
Experimental evaluation of probiotics as biocontrol/bioremediation in the rearing of shrimp (Penaeus monodon)
; ; Tendencia, Eleonor (Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Two hundred fifty-liter (250-L) fiberglass tanks each equipped with 15-L capacity filter tanks are adequate for screening of commercial probiotics. The application of some commercial probiotics in the culture water is more ...
Use of bacteria as biological control agent against microbial diseases in shrimp (Penaeus monodon) and crab (Scylla serrata) hatcheries: Part I. Isolation and selection of bacteria for biological control in crustacean hatcheries
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
A study aimed at obtaining a biological control agent against bacterial diseases of hatchery-reared shrimps and crabs was conducted. Bacteria were isolated from crustacean rearing environments where luminescent vibriosis ...
Development of monoclonal antibody (MAb) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Antibody-based detection methods are relatively simple and rapid, aside from being specific and sensitive. Thus, there is a necessity to develop such techniques for early and rapid diagnosis of viral pathogens such as MBV ...