Now showing items 74-79 of 79

    • Oral presentation

      Thermal tolerance of larval greentail prawn Metapenaeus bennettae (Raced and Dall) a comparison with school prawn Metapenaeus macleayi. 

      T Murai - In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development Center
      The thermal tolerance of four larval stages of Metapenaeus bennettae was studied in the laboratory. Critical Thermal Maximum (CTM), One hour Median Lethal Temperature (lhLT50), and Median Resistance Time (MRT) were measured. Moulting rate of larvae and hatching rate of embryos were also monitored to study the delayed effect of thermal stress.

      Thermal tolerance was shown to be strongly dependent on acclimation temperature (TA) at all larval stages,which showed ontogenetic development of thermal resistance. Moulting of larvae was hindered at temperatures (37.2°C for nauplius when TA=25°C) well below lhLT50 (38.1°C for nauplius when TA=25°C). The embryonic stages were more susceptible to thermal stress than the larval stages. The salinity effects were also significant. Nauplius and protozoea stages showed their highest CTM values at the salinity in which they were spawned.When compared with another penaeid M. macleayi (offshore breeder), M. bennettae (estuarine breeder) was found to have higher thermal resistance, but was less adaptive to changes in acclimation temperature.
    • Conference poster

      The tolerance of Penaeus monodon eggs and larvae to fungicides against Lagenidium sp. and Haliphthoros philippinensis. 

      GL Po & E Sanvictores - In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development Center
      The in vivo effect of mycostatic levels of fungicides against the fungi Lagenidium sp. and Haliphthoros sp. were tested on Penaeus monodon eggs and larvae. Hatching rate and survival of nauplii, zoeae, myses and postlarvae exposed to 10 mg/ℓ Benzalkonium chloride, 1 mg/ℓ Clotrimazole, 1 mg/ℓ Crystal Violet, 10 mg/ℓ 2,4-D, 10 mg/ℓ Daconil, 20 mg/ℓ laundry detergent, 1 mg/ℓ Econazole nitrate, 10 mg/ℓ Resiguard, 0.2 mg/ℓ and 10 mg/ℓ Treflan-R, 0.01 mg/ℓ and 0.2 mg/ℓ Trifluralin were monitored daily for 96 hr in a static bioassay in glass aquaria. Results showed that all test chemicals had no inhibitory effect on hatching rate but survival rate of hatched nauplii was significantly reduced in most treatments except that of 0.2 mg/ℓ Treflan-R. Tests with zoeae, myses and postlarvae indicated that 0.2 mg/ℓ Treflan-R and 0.01 mg/ℓ and 0.2 mg/ℓ Trifluralin did not adversely affect survival. In addition, Benzalkonium chloride caused no significant mortalities among exposed myses.
    • Conference poster

      The use of haptophyceae in rearing experiments on larval Penaeus orientalis. 

      MR Li, BZ Bian, L Ma & L Ma - In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development Center
      The food value of five clones of Haptophyceae, Coccolithus pelagicus, Dicrateria zhanjiangensis, Isochrysis galbana, Tahitian Isochrysis aff. galbana, and Pseudoisochrysis paradoxa were tested for larval Penaeus orientalis. The algae were semi-continuously cultured in 5,000 ml carboys with 4,000 ml of Guillard f/2 medium, under 2,000 lux continuous light and under aeration. The algal density was up to 1 × 107 cell/ml. Rearing experiments were conducted in round tanks with diameter of 45 cm. Algal density was controlled at 1 × 105 cell/ml in the course of the experiments. The larval density was 18 individual/100 ml; water temperature, 21-24°C; pH, 7.5-7.7; and sea water specific gravity, 1.019.

      The results showed that of five clones used, Tahitian I. aff. galbana and D. zhangjiangensis proved to be the best. It took 9-11 days for nauplius I to develop into mysis I with survival rate of 73.5% and 73.4%, respectively.
    • Oral presentation

      The use of microencapsulated feeds to replace live food organisms in shrimp hatcheries. 

      ED Scura, J Fischer & MP Yunker - In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development Center
      An adequate supply of hatchery produced shrimp fry is the major constraint to the intensification and growth of shrimp culture practices. If even 20% of the more than 500,000 ha of the world's existing tropical and sub-tropical brackishwater ponds were to stock at the relatively low density of 50,000 fry/ha/year, it would take thousands of new hatcheries to produce the 25 billion fry required. The availability of artificially produced diets to replace cultured live food organisms would alleviate many of the problems currently limiting shrimp hatchery production by: (i) reducing the level of technical skill required to operate a hatchery; (ii) assuring a reliable supply of a nutritionally balanced larval feed; (iii) reducing sources of contamination and larval disease; and (iv) simplifying hatchery design and capital cost requirements, thereby facilitating small scale hatchery development.

      Aquatic farms has been working with the Mars Microencapsulation Research Group (MMRG) to develop techniques for adapting current shrimp hatchery technology and design so that MMRG feeds can be used in existing hatcheries as a live feed replacement. Feeding trials have been conducted in commercial hatcheries in Hawaii, Malaysia and Thailand. The results of these trials and the techniques employed are discussed. Growth and survival of larvae fed microencapsulated diets as total or partial replacement of live foods was comparable to larvae cultured in control tanks using the standard operating procedures of the hatchery in which the trials were conducted. In trials to date, larval survival from nauplii to postlarvae has been as high as 70%.
    • Oral presentation

      Variation in tissue lipid content and fatty acid composition during ovarian maturation of unablated and ablated Penaeus monodon broodstock. 

      OM Millamena, R Pudadera & MR Catacutan - In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development Center
      The tissue lipid content and fatty acid composition in the hepatopancreas, tail muscle and gonad of unablated and ablated Penaeus monodon were determined. Females at various stages of maturity were collected from offshore spawning grounds in Tigbauan and Guimbal, Iloilo, Philippines. Ablated females were reared in captivity.

      The hepatopancreas showed the highest lipid content at 15.72 to 25.20% in unablated females and 22.47 to 34.90% in ablated females. Fresh lipid levels averaged 2.60% with no marked variation throughout the maturation period. Ovarian lipid increased from 5.80% (unablated) and 7.50% (ablated) in Immature Ovaries to more than two-fold in Early Maturing Ovaries coupled with a drop in hepatopancreatic lipid suggesting lipid mobilization to the ovaries. In ablated females, ovarian lipid progressively increased to a maximum of 21.90% in Fully Mature Ovaries with a corresponding rise in hepatopancreatic lipid. Both the ovarian and hepatopancreatic lipids declined in spent females. Fatty acid profiles of the tissues consistently showed the presence of polyunsaturated fatty acids (PUFA) 20:4ω6, 20:5ω3 and 22:6ω3. These fatty acids were reflected in the spawned egg. The lipid level in the hepatopancreas appeared to be inversely related to the total PUFA concentration in the ovaries. Lipid accumulation in ablated females was significantly higher than in unablated females.

      The findings suggest storage and subsequent utilization of lipids for maturation and spawning processes. The type of polyunsaturates present in the maturing ovaries is indicative of their metabolic and physiological importance in the reproductive process.
    • Oral presentation

      Water quality criteria for farming the grass shrimp, Penaeus monodon. 

      HC Chen - In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development Center
      Physiological and growth effects of pH, salinity, temperature, heavy metals, pesticides and others on juvenile grass shrimp Penaeus monodon have been investigated to

      determine the biologically safe concentrations. Optimal pH, salinity and temperature are found to be in the range of 8.0-8.5, 15-25 ppt, and 28-33°C, respectively. A dissolved oxygen concentration of 3.7 ppm seems to be the critical oxygen pressure to support the normal life of grass shrimp. To avoid poor survival and retarded growth, the recommended level for each pollutant are: heavy metals, 0.0025 ppm Hg, 0.1 ppm Cu, 0.15 ppm Cd, 0.25 ppm Zn; pesticides, 0.0004 ppb parathion, 0.001 ppb malathion, 0.008 ppb rotenone, 0.01 ppb Azodrin, 0.033 ppb Saturn, 0.01 ppb paraquat, 0.01 ppb Endosulfan, 1 ppb Butachlor; surfactants, 0.1 ppm Dunall OSE, 0.2 ppm BP 1100, 0.5 ppm Seagreen 805; and others, 0.033 ppm H2S, 0.1 ppm NH3.