Browsing Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps by Title
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Variation in tissue lipid content and fatty acid composition during ovarian maturation of unablated and ablated Penaeus monodon broodstock. - In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe tissue lipid content and fatty acid composition in the hepatopancreas, tail muscle and gonad of unablated and ablated Penaeus monodon were determined. Females at various stages of maturity were collected from offshore spawning grounds in Tigbauan and Guimbal, Iloilo, Philippines. Ablated females were reared in captivity. The hepatopancreas showed the highest lipid content at 15.72 to 25.20% in unablated females and 22.47 to 34.90% in ablated females. Fresh lipid levels averaged 2.60% with no marked variation throughout the maturation period. Ovarian lipid increased from 5.80% (unablated) and 7.50% (ablated) in Immature Ovaries to more than two-fold in Early Maturing Ovaries coupled with a drop in hepatopancreatic lipid suggesting lipid mobilization to the ovaries. In ablated females, ovarian lipid progressively increased to a maximum of 21.90% in Fully Mature Ovaries with a corresponding rise in hepatopancreatic lipid. Both the ovarian and hepatopancreatic lipids declined in spent females. Fatty acid profiles of the tissues consistently showed the presence of polyunsaturated fatty acids (PUFA) 20:4ω6, 20:5ω3 and 22:6ω3. These fatty acids were reflected in the spawned egg. The lipid level in the hepatopancreas appeared to be inversely related to the total PUFA concentration in the ovaries. Lipid accumulation in ablated females was significantly higher than in unablated females. The findings suggest storage and subsequent utilization of lipids for maturation and spawning processes. The type of polyunsaturates present in the maturing ovaries is indicative of their metabolic and physiological importance in the reproductive process.
Oral presentation- In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development CenterPhysiological and growth effects of pH, salinity, temperature, heavy metals, pesticides and others on juvenile grass shrimp Penaeus monodon have been investigated to determine the biologically safe concentrations. Optimal pH, salinity and temperature are found to be in the range of 8.0-8.5, 15-25 ppt, and 28-33°C, respectively. A dissolved oxygen concentration of 3.7 ppm seems to be the critical oxygen pressure to support the normal life of grass shrimp. To avoid poor survival and retarded growth, the recommended level for each pollutant are: heavy metals, 0.0025 ppm Hg, 0.1 ppm Cu, 0.15 ppm Cd, 0.25 ppm Zn; pesticides, 0.0004 ppb parathion, 0.001 ppb malathion, 0.008 ppb rotenone, 0.01 ppb Azodrin, 0.033 ppb Saturn, 0.01 ppb paraquat, 0.01 ppb Endosulfan, 1 ppb Butachlor; surfactants, 0.1 ppm Dunall OSE, 0.2 ppm BP 1100, 0.5 ppm Seagreen 805; and others, 0.033 ppm H2S, 0.1 ppm NH3.