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dc.contributor.authorMonghit-Camarin, Ma-ann
dc.contributor.authorCruz-Lacierda, Erlinda R.
dc.contributor.authorPakingking, Rolando V., Jr.
dc.contributor.authorCuvin-Aralar, Maria Lourdes
dc.contributor.authorTraifalgar, Rex Ferdinand
dc.contributor.authorAñasco, Nathaniel C.
dc.contributor.authorAustin, Frank William
dc.contributor.authorLawrence, Mark Lee
dc.contributor.editorFerrer, Alice Joan
dc.date.accessioned2023-03-10T08:07:15Z
dc.date.available2023-03-10T08:07:15Z
dc.date.issued2022
dc.identifier.citationMonghit-Camarin, M., Cruz-Lacierda, E., Pakingking, Jr., R. V., Cuvin-Aralar, M. L., Traifalgar, R. F. M., Añasco, N. C., Austin, F. W., & Lawrence, M. L. (2022). Bacterial microbiota of hatchery-reared freshwater prawn Macrobrachium rosenbergii (De Man, 1879) and their pathogenicity. In A. J. G. Ferrer (Ed.), Proceeding of Joint International Conference of the Sixth International Conference on Fisheries and Aquatic Sciences (ICFAS 6) and Asian Fisheries Social Science Research Network Forum One (AFSSRN F1), 24-26 November 2021, via Zoom, Miagao, Iloilo, Philippines (pp. 209–215). University of the Philippines Visayas.en
dc.identifier.isbn9786218285125
dc.identifier.urihttp://hdl.handle.net/10862/6418
dc.description.abstractQuantitative and qualitative analyses of bacterial microbiota associated with hatchery of Macrobrachium rosenbergii (de Man, 1879) were conducted over three larval cycles, along with important water quality parameters. Physicochemical parameters (temperature, DO, salinity, pH, and total ammonia) of culture water were within the optimum level required for the hatchery phase of M. rosenbergii. Significant differences in aerobic plate count (APC) among larval stages were observed. Ten genera and 17 species were identified with the predominance of Gram-negative bacteria constituting 63 % of all isolates (n = 706). A total of 17 dominant bacterial isolates randomly selected from freshwater prawn larvae were screened for in vitro hemolysin activity using blood agar plate (BAP) medium with 5% sheep blood. Pathogenicity of the isolated bacterial strains was assessed through in vivo infection bioassay. Out of the 17 isolates tested, six isolates showed α – β zone of hemolysis on the BAP, i.e., Aeromonas caviae, Bacillus cereus, Plesiomonas shigelloides, Staphylococcus warneri, Vibrio cholerae non-01, and Vibrio furnissii, were used in the infection bioassay. The current data indicate that the six bacterial isolates tested, particularly B. cereus and V. cholerae non-01, are opportunistic pathogens capable of inducing disease epizootics, particularly in freshwater prawn hatcheries with suboptimal rearing water conditions. Thus, control measures to reduce the influx of pathogenic microbes in the system by maintaining good water quality and good farm management practices through disinfection of culture facilities, rearing water, and Artemia cysts, the practice of good hygiene of personnel, regular water exchange, and feed regulation, among others, and cautionary use of antibiotics, can be adopted.en
dc.language.isoenen
dc.publisherUniversity of the Philippines Visayasen
dc.subjectMacrobrachium rosenbergiien
dc.subjectBacillus cereusen
dc.subjectPlesiomonas shigelloidesen
dc.subjectVibrio choleraeen
dc.subjectArtemiaen
dc.titleBacterial microbiota of hatchery-reared freshwater prawn Macrobrachium rosenbergii (De Man, 1879) and their pathogenicityen
dc.typeConference paperen
dc.citation.spage209en
dc.citation.epage215en
dc.citation.conferenceTitleProceeding of Joint International Conference of the Sixth International Conference on Fisheries and Aquatic Sciences (ICFAS 6) and Asian Fisheries Social Science Research Network Forum One (AFSSRN F1), 24-26 November 2021, via Zoom, Miagao, Iloilo, Philippinesen
dc.subject.asfaBacteriaen
dc.subject.asfashrimp fisheriesen
dc.subject.asfapathogenic bacteriaen
dc.subject.asfapathogensen
dc.subject.asfaculture tanksen
dc.subject.asfawater temperatureen
dc.subject.asfadissolved oxygenen
dc.subject.asfapHen
dc.subject.asfasalinityen
dc.subject.asfaammoniaen
dc.subject.asfashrimp cultureen
dc.subject.asfawater qualityen
dc.subject.asfafloraen
dc.subject.asfaprawn cultureen
dc.subject.asfaaquacultureen
dc.subject.asfabacteriologyen
dc.subject.scientificNameMacrobrachium rosenbergiien
dc.subject.scientificNameAeromonas caviaeen
dc.subject.scientificNameBacillus cereusen
dc.subject.scientificNamePlesiomonas shigelloidesen
dc.subject.scientificNameStaphylococcus warnerien
dc.subject.scientificNameVibrio choleraeen
dc.subject.scientificNameVibrio furnissiien
dc.subject.scientificNameArtemiaen
local.subjectQuantitative analysisen
local.subjectspecies compositionen
local.subjectqualitativeen
local.subjectnormal floraen
local.subjectpathogenen


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