SEAFDEC/AQD Institutional Repository: Recent submissions
681-700 / 4207
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AQD Matters 2020 May - June
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2020-07)In this issue: a) Sandfish: expensive, endangered, and ecologically essential; b) Genetics expert joins regional workshop on aquatic genetic resources registry dev't; c) Mori completes term as AQD Deputy Chief; d) Expert ... -
Detection and identification of viral pathogens in marine finfish cultured in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)This is a summary of investigations conducted on 91 disease/mortality cases observed among marine fish: groupers (Epinephelus coioides, E. malabaricus), sea bass (Lates calcarifer), rabbitfish (Siganus spp.), red snapper ... -
Development of shrimp (Penaeus monodon) cell culture in vitro
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)Primary shrimp cell cultures were successfully developed from lymphoid organ of black tiger shrimp (Penaeus monodon) in double-strength Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum, 1% glucose, 15% ... -
Chlorine disinfection against hepatopancreatic parvovirus (HPV) infection in shrimp (Penaeus monodon) larvae
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)Experiments were undertaken to determine the efficacy of chlorine as a disinfectant in inhibiting HPV infection among P. monodon postlarvae (PLs). Based on histopathological examination, 50 ppm and 200 ppm active chlorine ... -
Production of polyclonal antibodies (PAbs) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) are two of the viral pathogens, which infect penaeid shrimps in the Philippines. Polyclonal antibodies (PAbs) to MBV and HPV were raised by immunization of ... -
Development of monoclonal antibody (MAb) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)Antibody-based detection methods are relatively simple and rapid, aside from being specific and sensitive. Thus, there is a necessity to develop such techniques for early and rapid diagnosis of viral pathogens such as MBV ... -
Studies on the experimental transmission model of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)Series of experiments on the transmission of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon were undertaken. Feeding of MBV-infected carcasses to experimental shrimps resulted to 100% ... -
Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) remain as two of the serious pathogens that infect black tiger shrimp (Penaeus monodon) in the Philippines. It is, therefore, necessary to prevent the spread ... -
Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)Several runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot ... -
Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia: General information
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)Research is the main component of the Regional Fish Disease Project supported by the Government of Japan (GOJ) Trust Fund. When the project started in 2000, research was undertaken by scientists of the SEAFDEC Aquaculture ... -
Background and objectives of the Regional Fish Disease Project
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Development of specific enzyme-linked immunosorbent assay for yellowtail kingfish (Seriola lalandi) follicle stimulating hormone using recombinant gonadotropins
; Palma, Peter
; ; ; ; (Elsevier, 2019)
We developed a specific competitive enzyme-linked immunosorbent assay (ELISA) for yellowtail kingfish (Seriola lalandi) follicle stimulating hormone (FSH). We previously produced a full-length single chain recombinant ... -
Aquaculture breeding enhancement: Maturation and spawning in sea cucumbers using a recombinant relaxin-like gonad-stimulating peptide
; ; ; ; ; Palma, Peter; Suwansa-ard, Saowaros ; ; (Frontiers Media, 2019-02-19)
Wild sea cucumber resources have been rapidly exhausted and therefore there is an urgent need to develop approaches that will help restocking. Currently, there is a lack of information regarding the genes involved in sea ... -
Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper
; ; Palma, Peter; ; ; (Elsevier, 2020-07-13)
A recombinant giant grouper Luteinizing Hormone (LH) consisting of tethered beta and alpha subunits was produced in a yeast expression system. The giant grouper LH β-subunit was also produced and administered to rabbits ... -
Value chain analysis of the horse mussel Modiolus metcalfei (Hanley, 1843) fishery in Iloilo, Philippines
(Asian Fisheries Society, 2020-06-30)The exploitation of horse mussels Modiolus metcalfei (Hanley, 1843) in Dumangas, Iloilo has been going on for decades, yet effective fisheries management remains hindered by the poor understanding of the fishery. A value ... -
In-vitro evaluation of some commercial probiotics used in the grow-out culture of the tiger shrimp Penaeus monodon: Effect on bacterial parameters
(Research India Publications, 2007)This study was conducted to evaluate 8 commercial probiotic products being marketed for the grow-out culture of the tiger shrimp Penaeus monodon and the “SEAFDEC Indigenous Probiotic”. Bacterial parameters investigated ... -
Series: Aquaculture extension manual; No. 67
Biology and hatchery rearing of the silver therapon Leiopotherapon plumbeus
Aya, FrolanKnown for its tasty flesh, silver therapon, locally known as ayungin, has been regarded to be one of the most valuable edible native freshwater species in the Philippines. Demands for this fish species remain high which ...; Garcia, Luis Maria (Aquaculture Department, Southeast Asian Fisheries Development Center, 2020-07)
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Series: Milkfish Podcasts
Milkfish aquaculture: Alternate-day feeding strategy for reducing cost of milkfish culture in brackishwater ponds and marine cages in the Philippines
(AquaFish Collaborative Research Support Program (AquaFish CRSP), 2012-02-02)This podcast provides information on how an alternate-day feeding strategy can be used to reduce costs for culturing milkfish in ponds or marine cages. -
Training on business planning and financial management to support the Sustainable Mariculture Investment Program
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2013-07)The Sustainable Mariculture Investment Program (SMIP) of the Development Bank of the Philippines being undertaken by the Southeast Asian Fisheries Development Center/Aquaculture Department (SEAFDEC/AQD) in partnership with ... -
Survey and assessment of mariculture activities in Panabo Mariculture Park (Davao del Norte), Tagabuli Bay and Malalag Bay (Davao del Sur)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2013-07)The Development Bank of the Philippines (DBP) embarked on a financing program called the Sustainable Mariculture Investment Program (SMIP) aimed to make credit and support facilities available for the establishment, ...
