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Molecular typing of Vibrio parahaemolyticus strains isolated from the Philippines by PCR-based methods

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Date
2005
Author
Maluping, R. P.
Ravelo, C.
Lavilla-Pitogo, C. R.
Krovacek, K.
Romalde, J. L.
Page views
1,740
ASFA keyword
nucleotide sequence ASFA
microbiological strains ASFA
DNA ASFA
polymerase chain reaction ASFA
genomes ASFA
genetic isolation ASFA
microbiology ASFA
AGROVOC keyword
Typing
random amplified polymorphic DNA AGROVOC
Conserved sequence
Vibrio parahaemolyticus AGROVOC
Penaeus monodon AGROVOC
Philippines AGROVOC
Giant tiger prawn
Enterobacterial repetitive intergenic consensus sequence PCR,
Repetitive extragenic palindromic PCR
Taxonomic term
Penaeus monodon GBIF
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  • CrossRef - Citation Indexes: 27
  • PubMed - Citation Indexes: 6
  • Scopus - Citation Indexes: 35
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Abstract
Aim: The main aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines.

Methods and Results: Seventeen strains of V. parahaemolyticus isolated from shrimps (Penaeus monodon) and from the environments where these shrimps are being cultivated were analysed by random amplified polymorphic DNA PCR (RAPD-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and repetitive extragenic palindromic PCR (REP-PCR). The results of this work have demonstrated genetic variability within the V. parahaemolyticus strains that were isolated from the Philippines. In addition, RAPD, ERIC and REP-PCR are suitable rapid typing methods for V. parahaemolyticus. All three methods have good discriminative ability and can be used as a rapid means of comparing V. parahaemolyticus strains for epidemiological investigation. Based on the results of this study, we could say that REP-PCR is inferior to RAPD and ERIC-PCR owing to the fact that it is less reproducible. Moreover, the REP-PCR analysis yielded a relatively small number of products. This may suggests that the REP sequences may not be widely distributed in the V. parahaemolyticus genome.

Conclusions: Genetic variability within V. parahaemolyticus strains isolated in the Philippines has been demonstrated. The presence of ERIC and REP sequences in the genome of this bacterial species was confirmed. Significance and Impact of the Study:The RAPD, ERIC and REP-PCR techniques are useful methods for molecular typing of V. parahaemolyticus strains. To our knowledge this is the first study of this kind carried out on V. parahaemolyticus strains isolated from the Philippines.
URI
http://hdl.handle.net/10862/1668
Suggested Citation
Maluping, R. P., Ravelo, C., Lavilla-Pitogo, C. R., Krovacek, K., & Romalde, J. L. (2005). Molecular typing of Vibrio parahaemolyticus strains isolated from the Philippines by PCR-based methods. Journal of Applied Microbiology, 99(2), 383-391. https://doi.org/10.1111/j.1365-2672.2005.02571.x 
DOI
10.1111/j.1365-2672.2005.02571.x
Type
Article
ISSN
1364-5072
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  • Journal Articles [1249]

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