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  • Breeding and Seed Production of Cultured Finfishes in the Philippines
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  • 01 SEAFDEC/AQD Publications
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  • Breeding and Seed Production of Cultured Finfishes in the Philippines
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LHRHa and pimozide-induced breeding in the catfish, Clarias macrocephalus (Gunther)

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bspcfp_p110a.pdf (42.22Kb) Open Access
Downloads: 1,741
Date
1996
Author
Tan-Fermin, J. D.
Gapasin, R. S. J.
Tan, A. M.
Garcia, M. A.
Emata, Arnil C.
Page views
5,849
ASFA keyword
endemic species ASFA
ovulation ASFA
sexual maturity ASFA
fish eggs ASFA
oocytes ASFA
standards ASFA
induced ovulation ASFA
freshwater fishes ASFA
fertilization ASFA
hatching ASFA
oogenesis ASFA
sex hormones ASFA
AGROVOC keyword
Clarias macrocephalus AGROVOC
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Abstract
Clarias macrocephalus is endemic yet dwindling freshwater foodfish in the Philippines. Induced breeding protocol was developed by monitoring the size and maturation of eggs at 0-48 h after a simultaneous injection of luteinizing hormone-releasing hormone analogue (LHRHa; 0.0005 - 0.10 µg/g BW) and pimozide (PIM; 1 µg/g BW). Based on its similar osmotic pressure with catfish plasma, eggs were fixed in 1% phosphate-buffered formalin. Mean egg diameter of fish that were induced to mature increased during ovulation. Oocyte maturation, indicated by oocytes with germinal vesicle breakdown (GVBD), was observed at least 12 h post-injection in fish given 0.01 - 0.10 µg LHRHa + 1 µg PIM/g BW, followed by ovulation 4 h thereafter. Results showed that a simultaneous injection of C. macrocephalus with 0.05 µg LHRHa + 1 µg PIM/g BW at 1800-1900 h followed by stripping at 16-20 h post-injection resulted in high ovulation, fertilization and hatching rates.
Description
Abstract only.
URI
http://hdl.handle.net/10862/569
Suggested Citation
Tan-Fermin, J. D. (1996). LHRHa and pimozide-induced breeding in the catfish, Clarias macrocephalus (Gunther). In C. L. Marte, G. F. Quinitio, & A. C. Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993 (p. 110). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Type
Conference paper
ISBN
9718511326
Collections
  • Breeding and Seed Production of Cultured Finfishes in the Philippines [31]


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