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Studies on the experimental transmission model of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Series of experiments on the transmission of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon were undertaken. Feeding of MBV-infected carcasses to experimental shrimps resulted to 100% ...
Development of immunological indices for monitoring health status in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Some hemolymph parameters were determined as non-invasive early-warning indicators of health status in black tiger shrimp (Penaeus monodon). These included total hemocyte count (THC), respiratory burst activity, phenoloxidase ...
Parasites of commercially-important marine fish cultured in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
The commercially-important cultured marine fish, orange-spotted grouper (Epinephelus coioides), mangrove red snapper (Lutjanus argentimaculatus) and rabbitfish (Siganus guttatus), were examined at regular intervals to ...
Prevalence of economically-important viral diseases in wild shrimp (Penaeus monodon) in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Prevalences of white spot syndrome virus (WSSV) and monodon baculovirus (MBV) were determined using polymerase chain reaction (PCR) as the detection method in wild black tiger shrimp (Penaeus monodon) collected from seven ...
Standardization of PCR techniques as the detection method for white spot syndrome virus (WSSV) infection in shrimp (Penaeus monodon)
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Several runs were conducted using the three published PCR protocols and the three DNA extraction methods. All three primer pairs designed by individual authors based on the sequence of a cloned fragment of the white spot ...
Important parasites of freshwater fish in Thailand and neighboring countries
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Snakehead fish (Chana striata) from Thailand and Cambodia were sampled for parasitological study. The project was carried out from August 2003 to January 2004 twice a month. The results showed that there were 22 genera ...
Important parasites of catfish (Clarias macrocephalus) in the Philippines
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
The parasite fauna of broad head catfish (Clarias macrocephalus) was determined through regular examination of cultured and wild-caught fish. Collection areas included hatchery, nursery and grow-out facilities in Iloilo ...
Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia: General information
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Research is the main component of the Regional Fish Disease Project supported by the Government of Japan (GOJ) Trust Fund. When the project started in 2000, research was undertaken by scientists of the SEAFDEC Aquaculture ...
Detection and identification of iridoviral pathogens of freshwater fish in Thailand
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
An iridovirus or ranavirus survey was conducted in Thailand during April 2003 to March 2004. Five hundred and sixty-six diseased plus healthy fish and frog specimens were examined for the possibility of ranavirus infection ...
Development of shrimp (Penaeus monodon) cell culture in vitro
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
Primary shrimp cell cultures were successfully developed from lymphoid organ of black tiger shrimp (Penaeus monodon) in double-strength Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum, 1% glucose, 15% ...