SEAFDEC/AQDINSTITUTIONAL REPOSITORY
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • ไทย 
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • เข้าสู่ระบบ
ดูรายการ 
  •   SAIR บ้าน
  • 02 SEAFDEC/AQD Collaborative Publications
  • SEAFDEC/AQD-Government of Japan-Trust Fund (GOJ-TF)
  • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia
  • ดูรายการ
  •   SAIR บ้าน
  • 02 SEAFDEC/AQD Collaborative Publications
  • SEAFDEC/AQD-Government of Japan-Trust Fund (GOJ-TF)
  • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia
  • ดูรายการ
JavaScript is disabled for your browser. Some features of this site may not work without it.

Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV

  • Global styles
  • MLA
  • Vancouver
  • Elsevier - Harvard
  • APA
  • Help
Thumbnail
ดู/เปิด
  Preview (Limited to 10 pages)
CatapES2005.pdf (158.1Kb) AQD Access AQD Access
Downloads: 9
วันที่
2005-03
ผู้เขียน
Catap, Elena S.
de la Peña, Leobert D.
Page views
1,564
ASFA keyword
monoclonal antibodies ASFA
disease recognition ASFA
ELISA ASFA
animal diseases ASFA
AGROVOC keyword
Baculovirus AGROVOC
Penaeus monodon AGROVOC
polyclonal antibodies AGROVOC
Taxonomic term
Penaeus monodon GBIF
เมตาดาต้า
แสดงระเบียนรายการเต็ม
Share 
 
นามธรรม
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) remain as two of the serious pathogens that infect black tiger shrimp (Penaeus monodon) in the Philippines. It is, therefore, necessary to prevent the spread and occurrence of such pathogens, especially in shrimp hatcheries where postlarvae (PLs) stocked in culture ponds are usually obtained. One effective means is to establish rapid and reliable diagnostic techniques for these viruses.

This research was undertaken mainly to develop monoclonal antibodies (MAbs) against MBV and HPV. Consequently, these MAbs will be used as primary reagents to establish an appropriate immunologically-based detection method for MBV and HPV. Immunologically-based diagnostic methods have proven to be rapid, sensitive and quite specific. Furthermore, they could be performed in the field using very simple set-up.

Four main studies were conducted for this research. The first involves the development of infection model of HPV in P. monodon. Series of experiments showed that HPV could be horizontally-transmitted in postlarval P. monodon through the oral route. This allowed the availability of infected materials to be used as immunogen and for screening of antibodies. The infection model also provided a method by which the pathogen could be further studied, including its control in hatchery conditions. The second study describes the development of MAbs against HPV and MBV, including the optimization of an immunofluourescent antibody test (IFAT), as a screening technique. The third study involves the production of polyclonal antibodies (PAbs) against MBV and HPV wherein IFAT, immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA) were optimized and used to screen the antibodies as to their usefulness in MBV and HPV diagnosis. The fourth set of experiments were undertaken to determine the efficiency of chlorine as a disinfectant in inhibiting HPV infection in P. monodon PLs. The results of each study are discussed in this report.
URI
http://hdl.handle.net/10862/5908
การอ้างอิง
Catap, E. S., & de la Peña, L. D. (2005). Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV. In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 27–28). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Type
Book chapter
ISBN
9718511732
คอลเลกชัน
  • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia [43]

Related items

Showing items related by title, author, creator and subject.

  • Thumbnail

    Development of monoclonal antibody (MAb) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) 

    Catap, Elena S.; de la Peña, Leobert D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
    Antibody-based detection methods are relatively simple and rapid, aside from being specific and sensitive. Thus, there is a necessity to develop such techniques for early and rapid diagnosis of viral pathogens such as MBV ...
  • Thumbnail

    Production of polyclonal antibodies (PAbs) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) 

    Catap, Elena S.; de la Peña, Leobert D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
    Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) are two of the viral pathogens, which infect penaeid shrimps in the Philippines. Polyclonal antibodies (PAbs) to MBV and HPV were raised by immunization of ...
  • Thumbnail

    Immunological and molecular biology techniques in disease diagnosis 

    de la Peña, Leobert D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2001)
    The improvement of existing immunoassay techniques, development of monoclonal antibody technology and the development of new immunoassay approaches are all working together to provide new tools for the detection of ...

© SEAFDEC/AQD  2025
ส่งความคิดเห็น | Subscribe
 

 

หมวด

ทั้งหมดของ SAIRชุมชนและคอลเล็กชันตามวันที่ออกผู้เขียนชื่อเรื่องอาสาสมัครคอลเลกชันนี้ตามวันที่ออกผู้เขียนชื่อเรื่องอาสาสมัคร

บัญชีของฉัน

เข้าสู่ระบบRegister

Statistics

View Usage Statistics

© SEAFDEC/AQD  2025
ส่งความคิดเห็น | Subscribe
 

 

Export citations

Export the current results of the search query as a citation list. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

The list of citations that can be exported is limited to items.

Export citations

Export the current item as a citation. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

Export Citations

DOCUMENT REQUEST NOT AVAILABLE

This publication is still available (in PRINT) and for sale at AQD bookstore. The library is currently restricted to send PDF of publications that are still for sale.

You may contact bookstore@seafdec.org.ph or visit AQD bookstore for orders.

FILE UNDER EMBARGO

This file associated with this publication is currently under embargo. This will be available for download after the embargo date.