SEAFDEC/AQDINSTITUTIONAL REPOSITORY
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • English 
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • Login
View Item 
  •   SEAFDEC/AQD Institutional Repository Home
  • 02 SEAFDEC/AQD Collaborative Publications
  • SEAFDEC/AQD-Government of Japan-Trust Fund (GOJ-TF)
  • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia
  • View Item
  •   SEAFDEC/AQD Institutional Repository Home
  • 02 SEAFDEC/AQD Collaborative Publications
  • SEAFDEC/AQD-Government of Japan-Trust Fund (GOJ-TF)
  • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Production of polyclonal antibodies (PAbs) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV)

  • Global styles
  • MLA
  • Vancouver
  • Elsevier - Harvard
  • APA
  • Help
Thumbnail
View/Open
  Preview (Limited to 10 pages)
CatapES2005.pdf (971.1Kb) AQD Access AQD Access
Downloads: 4
Date
2005-03
Author
Catap, Elena S.
de la Peña, Leobert D.
Page views
1,899
ASFA keyword
animal diseases ASFA
shrimp culture ASFA
viral diseases ASFA
infectious diseases ASFA
disease recognition ASFA
DNA ASFA
monoclonal antibodies ASFA
immunization ASFA
immunofluorescence ASFA
AGROVOC keyword
polyclonal antibodies AGROVOC
Baculovirus AGROVOC
Penaeus monodon AGROVOC
Taxonomic term
Penaeus monodon GBIF
Metadata
Show full item record
Share 
 
Abstract
Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) are two of the viral pathogens, which infect penaeid shrimps in the Philippines. Polyclonal antibodies (PAbs) to MBV and HPV were raised by immunization of rabbits with the purified virions. The antisera produced were assessed using immunofluorescence antibody test (IFAT), indirect enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC). IFAT showed intense fluorescent reactions in impression smears and paraffin-embedded sections of MBV-infected P. monodon postlarvae (PLs) but not in smears and/or sections infected with HPV or WSSV. Similarly, IHC tests showed positive coloration in MBV-infected cells but not in sections with either HPV or WSSV. Indirect ELISA test revealed that the PAbs could detect 4 ng (rabbit 1) and 100 ng (rabbit 2) of purified MBV. However, testing of crude shrimp homogenates by indirect ELISA indicated high level cross-reactions (gills of WSSV-infected adult P. monodon, head portions of HPV- and MBV-infected PLs and non-infected PLs). Screening of anti-HPV serum likewise showed intense immunofluorescence and positive red brownish coloration in HPV-infected sections. However, there were also strong cross-reactions observed in sections of hepatopancreas with MBV infection. Overall, the MBV PAbs developed have potential application in the detection of the virus. Further purification of the PAbs is required to minimize background and/or cross-reactions.
URI
http://hdl.handle.net/10862/5911
Suggested Citation
Catap, E. S., & de la Peña, L. D. (2005). Production of polyclonal antibodies (PAbs) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV). In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 45–50). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Type
Book chapter
ISBN
9718511732
Collections
  • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia [43]

Related items

Showing items related by title, author, creator and subject.

  • Thumbnail

    Standardization of diagnostic methods for monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV): Establishment of monoclonal antibodies (MAbs) against MBV and HPV 

    Catap, Elena S.; de la Peña, Leobert D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
    Monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) remain as two of the serious pathogens that infect black tiger shrimp (Penaeus monodon) in the Philippines. It is, therefore, necessary to prevent the spread ...
  • Thumbnail

    Development of monoclonal antibody (MAb) against monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) 

    Catap, Elena S.; de la Peña, Leobert D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2005-03)
    Antibody-based detection methods are relatively simple and rapid, aside from being specific and sensitive. Thus, there is a necessity to develop such techniques for early and rapid diagnosis of viral pathogens such as MBV ...
  • Thumbnail

    Immunological and molecular biology techniques in disease diagnosis 

    de la Peña, Leobert D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2001)
    The improvement of existing immunoassay techniques, development of monoclonal antibody technology and the development of new immunoassay approaches are all working together to provide new tools for the detection of ...

© SEAFDEC/AQD  2025
Send Feedback | Subscribe
 

 

Browse

All of SAIRCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

Statistics

View Usage Statistics

© SEAFDEC/AQD  2025
Send Feedback | Subscribe
 

 

Export citations

Export the current results of the search query as a citation list. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

The list of citations that can be exported is limited to items.

Export citations

Export the current item as a citation. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

Export Citations

DOCUMENT REQUEST NOT AVAILABLE

This publication is still available (in PRINT) and for sale at AQD bookstore. The library is currently restricted to send PDF of publications that are still for sale.

You may contact bookstore@seafdec.org.ph or visit AQD bookstore for orders.

FILE UNDER EMBARGO

This file associated with this publication is currently under embargo. This will be available for download after the embargo date.