In vitro investigation of the anti-koi herpesvirus (KHV) effect of different disinfectants

Abstract

One cause of the rapid spread of koi herpesvirus (KHV) is the trade of live ornamental fish. One way of preventing the spread of the disease is through proper hygiene. This study was done to investigate the virucidal effects of some disinfectants on KHV in vitro. Results of the experiment have shown that KHV (TCID₅₀ = 10^7.5) exposed to different concentrations of iodine (3, 6, 9 %), benzalkonium chloride (3, 6, 9, 12, 15 %), formalin (0.1, 0.5, 1, 1.5, 2.0%) and hydrogen peroxide (3, 6, 9, 12, 15 %) did not produce cytopathic effect (CPE) on KF-1 cell lines after 7 days incubation at 20°C. Syncitia was observed in cell lines inoculated with KHV exposed to 12 and 15% iodine for 30 min.