Genome analysis of Pasteurella piscicida

Abstract

Genome analysis was conducted to detect the virulence genes in Pasteurella piscicida (Photobacterium damsela subsp. piscicida) KP9038. To date, we have analyzed 366 clones and determined 333,341 bp nucleotide sequences. As a result of comparison of these clones to the GenBank database, these clones were separated into functional categories; small-molecule metabolism, broad regulatory functions, macro-molecule metabolism, cell processes and others. At present, five clones from the genome analysis have been deduced to have homology with reported virulence genes. These are DsbA (required for enterotoxin biogenesis), nucleotide sugar epimerase, OpsX (lipopolysaccharide biosynthesis), LspA2 (filamentous hemagglutinin) and PldA (outer membrane phospholipase A).