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Production and characterization of a monoclonal antibody against Aeromonas hydrophila

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Date
2002
Author
Chanphong, Jitkasem
Chinabut, Supranee
Polchana, Jaree
Thompson, Kim D.
Adams, Alexandra
Page views
35
Subject
monoclonal antibodies ASFA
freshwater fishes ASFA
antigens ASFA
immunization ASFA
Aeromonas hydrophila AGROVOC
immunoglobulins AGROVOC
hybridomas AGROVOC
Taxonomic term
Osphronemus gourami GBIF
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Abstract
A monoclonal antibody (MAb), 8D10, was produced against Aeromonas hydrophila strain 94030 isolated from giant gourami (Osphronemus gourami). This was classified as an IgG2a immunoglobulin by ELISA, and recognised a 41 kDa band on whole cell preparations of the A. hydrophila by Western blotting. The reactivity of the MAb against twenty-five different A. hydrophila isolates was examined by an indirect ELISA and Western blotting. Twelve other bacterial species were also tested by the indirect ELISA to examine the specificity of the MAb. One of the A. hydrophila isolate (strain 95014) gave a strong reaction (89.9%) with the MAb in the ELISA and also recognised the 41 kDa band. Sixteen other strains were weak positive in the ELISA, and eleven of these strains were weakly positive by Western blotting. No cross-reaction with the non-A. hydrophila isolates occurred.
URI
http://hdl.handle.net/10862/6227
Suggested Citation
Chanphong, J., Chinabut, S., Polchana, J., Thompson, K. D., & Adams, A. (2002). Production and characterization of a monoclonal antibody against Aeromonas hydrophila. In C. R. Lavilla-Pitogo & E. R. Cruz-Lacierda (Eds.), Diseases in Asian aquaculture IV: Proceedings of the Fourth Symposium on Diseases in Asian Aquaculture, 22-26 November 1999, Cebu City, Philippines (pp. 355-360). Fish Health Section, Asian Fisheries Society.
Type
Conference paper
ISBN
9718020160
Collections
  • Diseases in Asian aquaculture IV [43]


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